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Article

Radix Dipsaci total saponins stimulate MC3T3-E1 cell differentiation via the bone morphogenetic protein-2/MAPK/Smad-dependent Runx2 pathway

  • Authors:
    • Yin‑Bo Niu
    • Xiang‑He Kong
    • Yu‑Hua Li
    • Li Fan
    • Ya‑Lei Pan
    • Chen‑Rui Li
    • Xiang‑Long Wu
    • Ting‑Li Lu
    • Qi‑Bing Mei
  • View Affiliations / Copyright

    Affiliations: Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi'an, Shaanxi 710072, P.R. China, Graduate School of Chang'an University, Xi'an, Shaanxi 710064, P.R. China, Department of Pharmacology, School of Pharmacy, Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China
  • Pages: 4468-4472
    |
    Published online on: January 26, 2015
       https://doi.org/10.3892/mmr.2015.3249
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Abstract

Radix Dipsaci total saponins (RTS) are primary active components of Radix Dipsaci, which is administered orally for the treatment of osteoporosis according to Chinese Medicine. RTS have also been shown to reduce the risk of bone fractures in rats. However, the detailed molecular mechanisms underlying their action remain elusive. In the present study, the ability of RTS to increase alkaline phosphatase activity, osteocalcin levels and the degree of mineralization was investigated in MC3T3‑E1 mouse osteoblast precursor cells. In addition, the associated molecular mechanism was detected. The results revealed that RTS exerted an effect on osteoblastic maturation and differentiation. Induction of differentiation by RTS was associated with an increase in the expression levels of bone morphogenetic protein‑2 (BMP‑2), phosphorylated (P)‑Smad1/5/8, P‑ERK1/2, P‑p38 and Runt‑related transcription factor 2 (Runx2). Blocking BMP‑2 expression with noggin significantly reduced the levels of osteoblastic differentiation and subsequently attenuated the expression levels of P‑Smad1/5/8, P‑ERK1/2, P‑p38 and Runx2. This indicated that RTS induced osteoblastic differentiation through BMP‑2/mitogen‑activated protein kinase/Smad1/5/8‑dependent Runx2 signaling pathways and that it may be a promising agent for enhancing bone formation.
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Copy and paste a formatted citation
Spandidos Publications style
Niu YB, Kong XH, Li YH, Fan L, Pan YL, Li CR, Wu XL, Lu TL and Mei QB: Radix Dipsaci total saponins stimulate MC3T3-E1 cell differentiation via the bone morphogenetic protein-2/MAPK/Smad-dependent Runx2 pathway. Mol Med Rep 11: 4468-4472, 2015.
APA
Niu, Y., Kong, X., Li, Y., Fan, L., Pan, Y., Li, C. ... Mei, Q. (2015). Radix Dipsaci total saponins stimulate MC3T3-E1 cell differentiation via the bone morphogenetic protein-2/MAPK/Smad-dependent Runx2 pathway. Molecular Medicine Reports, 11, 4468-4472. https://doi.org/10.3892/mmr.2015.3249
MLA
Niu, Y., Kong, X., Li, Y., Fan, L., Pan, Y., Li, C., Wu, X., Lu, T., Mei, Q."Radix Dipsaci total saponins stimulate MC3T3-E1 cell differentiation via the bone morphogenetic protein-2/MAPK/Smad-dependent Runx2 pathway". Molecular Medicine Reports 11.6 (2015): 4468-4472.
Chicago
Niu, Y., Kong, X., Li, Y., Fan, L., Pan, Y., Li, C., Wu, X., Lu, T., Mei, Q."Radix Dipsaci total saponins stimulate MC3T3-E1 cell differentiation via the bone morphogenetic protein-2/MAPK/Smad-dependent Runx2 pathway". Molecular Medicine Reports 11, no. 6 (2015): 4468-4472. https://doi.org/10.3892/mmr.2015.3249
Copy and paste a formatted citation
x
Spandidos Publications style
Niu YB, Kong XH, Li YH, Fan L, Pan YL, Li CR, Wu XL, Lu TL and Mei QB: Radix Dipsaci total saponins stimulate MC3T3-E1 cell differentiation via the bone morphogenetic protein-2/MAPK/Smad-dependent Runx2 pathway. Mol Med Rep 11: 4468-4472, 2015.
APA
Niu, Y., Kong, X., Li, Y., Fan, L., Pan, Y., Li, C. ... Mei, Q. (2015). Radix Dipsaci total saponins stimulate MC3T3-E1 cell differentiation via the bone morphogenetic protein-2/MAPK/Smad-dependent Runx2 pathway. Molecular Medicine Reports, 11, 4468-4472. https://doi.org/10.3892/mmr.2015.3249
MLA
Niu, Y., Kong, X., Li, Y., Fan, L., Pan, Y., Li, C., Wu, X., Lu, T., Mei, Q."Radix Dipsaci total saponins stimulate MC3T3-E1 cell differentiation via the bone morphogenetic protein-2/MAPK/Smad-dependent Runx2 pathway". Molecular Medicine Reports 11.6 (2015): 4468-4472.
Chicago
Niu, Y., Kong, X., Li, Y., Fan, L., Pan, Y., Li, C., Wu, X., Lu, T., Mei, Q."Radix Dipsaci total saponins stimulate MC3T3-E1 cell differentiation via the bone morphogenetic protein-2/MAPK/Smad-dependent Runx2 pathway". Molecular Medicine Reports 11, no. 6 (2015): 4468-4472. https://doi.org/10.3892/mmr.2015.3249
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