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Article

Downregulation of high mobility group box 1 attenuates the severity of acute lung injury in endotoxemic mice

  • Authors:
    • Xiaojuan Zhang
    • Zhenggang Luan
    • Yingjian Liang
    • Yina Liu
    • Xiaochun Ma
  • View Affiliations / Copyright

    Affiliations: Intensive Care Unit, First Affiliated Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China
  • Pages: 4513-4517
    |
    Published online on: January 26, 2015
       https://doi.org/10.3892/mmr.2015.3251
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Abstract

High mobility group box 1 (HMGB1) is a systemic inflammation‑associated cytokine mediator. The aim of the present study was to examine the effect of the downregulation of HMGB1 in a lipopolysaccharide (LPS)‑induced mouse model of acute lung injury (ALI). It was identified that serum levels of tumor necrosis factor‑α and interleukin‑1β, lung myeloperoxidase activity and malondialdehyde content, as well as lung wet/dry weight ratios were all increased following LPS challenge. However, LPS‑mediated increases in these parameters were significantly downregulated in HMGB1 small interfering (si)RNA‑treated mice versus the negative control siRNA‑treated mice. In addition, the administration of HMGB1 siRNA in LPS‑treated mice resulted in a decreased DNA binding activity of nuclear factor‑κB (NF‑κB) in the lung. It was demonstrated that downregulation of HMGB1 decreases inflammation and the severity of sepsis associated with ALI, possibly via inhibiting the NF‑κB DNA‑binding activity. The present data support HMGB1 as a contributor to the pathogenesis of LPS‑induced sepsis and ALI.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang X, Luan Z, Liang Y, Liu Y and Ma X: Downregulation of high mobility group box 1 attenuates the severity of acute lung injury in endotoxemic mice. Mol Med Rep 11: 4513-4517, 2015.
APA
Zhang, X., Luan, Z., Liang, Y., Liu, Y., & Ma, X. (2015). Downregulation of high mobility group box 1 attenuates the severity of acute lung injury in endotoxemic mice. Molecular Medicine Reports, 11, 4513-4517. https://doi.org/10.3892/mmr.2015.3251
MLA
Zhang, X., Luan, Z., Liang, Y., Liu, Y., Ma, X."Downregulation of high mobility group box 1 attenuates the severity of acute lung injury in endotoxemic mice". Molecular Medicine Reports 11.6 (2015): 4513-4517.
Chicago
Zhang, X., Luan, Z., Liang, Y., Liu, Y., Ma, X."Downregulation of high mobility group box 1 attenuates the severity of acute lung injury in endotoxemic mice". Molecular Medicine Reports 11, no. 6 (2015): 4513-4517. https://doi.org/10.3892/mmr.2015.3251
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang X, Luan Z, Liang Y, Liu Y and Ma X: Downregulation of high mobility group box 1 attenuates the severity of acute lung injury in endotoxemic mice. Mol Med Rep 11: 4513-4517, 2015.
APA
Zhang, X., Luan, Z., Liang, Y., Liu, Y., & Ma, X. (2015). Downregulation of high mobility group box 1 attenuates the severity of acute lung injury in endotoxemic mice. Molecular Medicine Reports, 11, 4513-4517. https://doi.org/10.3892/mmr.2015.3251
MLA
Zhang, X., Luan, Z., Liang, Y., Liu, Y., Ma, X."Downregulation of high mobility group box 1 attenuates the severity of acute lung injury in endotoxemic mice". Molecular Medicine Reports 11.6 (2015): 4513-4517.
Chicago
Zhang, X., Luan, Z., Liang, Y., Liu, Y., Ma, X."Downregulation of high mobility group box 1 attenuates the severity of acute lung injury in endotoxemic mice". Molecular Medicine Reports 11, no. 6 (2015): 4513-4517. https://doi.org/10.3892/mmr.2015.3251
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