Neuronic autophagy contributes to p-connexin 43 degradation in hippocampal astrocytes following traumatic brain injury in rats

Sun,Li  Qian; Gao,Jun  Ling; Cui,Ying; Zhao,Man  Man; Jing,Xiao  Bin; Li,Ran; Tian,Yan  Xia; Cui,Jian  Zhong; Wu,Zhong-Xue

doi:10.3892/mmr.2015.3264

June-2015 Volume 11 Issue 6

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June-2015 Volume 11 Issue 6

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Article

Neuronic autophagy contributes to p-connexin 43 degradation in hippocampal astrocytes following traumatic brain injury in rats

Authors:
- Li Qian Sun
- Jun Ling Gao
- Ying Cui
- Man Man Zhao
- Xiao Bin Jing
- Ran Li
- Yan Xia Tian
- Jian Zhong Cui
- Zhong-Xue Wu
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Affiliations: Department of Interventional Neuroradiology, Beijing Neurosurgical Institute and Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, P.R. China, Department of Histology and Embryology, School of Basic Medical Science, Hebei United University, Tangshan, Hebei 063000, P.R. China, Department of Ophthalmology, Tangshan Ophthalmology Hospital, Tangshan, Hebei 063000, P.R. China, Department of Neurosurgery, Tangshan Gongren Hospital, Tangshan, Hebei 063000, P.R. China
Pages: 4419-4423
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Published online on: January 27, 2015

https://doi.org/10.3892/mmr.2015.3264
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Abstract

Connexins, gap junction proteins, have short half‑lives of only a few hours; therefore, degradation of these proteins can rapidly modulate their function. Autophagy is a type of degradation pathway that has been implicated in several diseases and was reported to be induced following traumatic brain injury (TBI). The aim of the present study was to investigate the involvement of neuronic autophagy in proteolysis of phosphorylated connexin 43 (p‑Cx43) in hippocampal astrocytes following TBI in rats. Western blot analysis and immunofluorescence showed a TBI‑induced increase in levels of astrocytic p‑Cx43 following treatment with 3‑methyladenine, an inhibitor of autophagy, in the hippocampus. Internalized gap junctions were observed in the neuronic cytoplasm using transmission electron microscopy. These results demonstrated that neuronic autophagy may regulate cellular levels of p‑Cx43 in hippocampal astrocytes following TBI. This therefore indicated that the persistence of p‑Cx43 accumulation was due to insufficient degradation capacity of constitutive autophagy.

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