microRNA‑299‑3p inhibits laryngeal cancer cell growth by targeting human telomerase reverse transcriptase mRNA

Li,Man; Chen,Shi‑Ming; Chen,Chen; Zhang,Zi‑Xiong; Dai,Meng‑Yuan; Zhang,Lei‑Bo; Wang,Shui‑Bin; Dai,Qiong; Tao,Ze‑Zhang

doi:10.3892/mmr.2015.3287

June-2015 Volume 11 Issue 6

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June-2015 Volume 11 Issue 6

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Article

microRNA‑299‑3p inhibits laryngeal cancer cell growth by targeting human telomerase reverse transcriptase mRNA

Authors:
- Man Li
- Shi‑Ming Chen
- Chen Chen
- Zi‑Xiong Zhang
- Meng‑Yuan Dai
- Lei‑Bo Zhang
- Shui‑Bin Wang
- Qiong Dai
- Ze‑Zhang Tao
View Affiliations / Copyright

Affiliations: Department of Otolaryngology‑Head and Neck Surgery, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China
Pages: 4645-4649
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Published online on: January 30, 2015

https://doi.org/10.3892/mmr.2015.3287
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Abstract

Aberrant microRNA (miRNA) expression has been linked to cancer development. In this study, we aimed to investigate whether the anti‑cancer effect of miRNA‑299‑3p on laryngeal cancer Hep‑2 cells is mediated through targeting human telomerase reverse transcriptase (hTERT). The expression of miR‑299‑3p in laryngeal cancer Hep‑2 cells and human osteosarcoma U2OS cells was quantified by stem‑loop‑mediated reverse transcription quantitative polymerase chain reaction. miR‑299‑3p mimic was transfected into Hep‑2 cells to induce overexpression of miR‑299‑3p. A CCK‑8 assay was performed to identify the effects of miR‑299‑3p overexpression on the proliferation of Hep‑2 cells. Western blot analysis was carried out to determine the expression of hTERT protein. A significant decrease was noted in the expression of miR‑299‑3p in Hep‑2 cells compared with that of U2OS cells (P<0.05). Overexpression of miR‑299‑3p resulted in a notable inhibition of cellular proliferation (P<0.05), as well as downregulation of hTERT mRNA and protein in Hep‑2 cells (P>0.05). The expression of miR‑299‑3p is downregulated in human laryngeal cancer Hep‑2 cells. Overexpression of miR‑299‑3p inhibits Hep‑2 cell growth by targeting the 3'‑untranslated region of hTERT mRNA.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

microRNA‑299‑3p inhibits laryngeal cancer cell growth by targeting human telomerase reverse transcriptase mRNA

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Abstract

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