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Molecular Medicine Reports
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June-2015 Volume 11 Issue 6

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Article

Side population cells and drug resistance in breast cancer

  • Authors:
    • Meijian Wang
    • Yaqi Wang
    • Jun Zhong
  • View Affiliations / Copyright

    Affiliations: Department of Oncology, Jiangxi Provincial Tumor Hospital, Nanchang, Jiangxi 330029, P.R. China
  • Pages: 4297-4302
    |
    Published online on: January 30, 2015
       https://doi.org/10.3892/mmr.2015.3291
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Abstract

Several studies have demonstrated that the isolated side population (SP) cells from solid tumors exhibit cancer stem cell‑like properties, and are responsible for drug resistance during chemotherapy and tumor recurrence. In the current study, cancer stem cell‑like SP cells were isolated in the MDU‑22 breast cancer cell line using the Hoechst‑33342 dye exclusion technique. It was observed that SP cells accounted for 3.8% of cells in the MDU‑22 cell line, which was reduced to 0.6% in the presence of verapamil, an inhibitor of the ABC transporter. The sorted SP cells showed an elevated expression of stem cell markers, including ABCG2, OCT‑4 and EpCAM. Furthermore, it was demonstrated that the isolated SP cells undergo rapid proliferation and have a high survival rate. These results indicate that the coexpression of adenosine triphosphatase binding cassette transporters and stem cell surface markers in SP cells may contribute to chemoresistance, tumor recurrence, metastasis and invasion. Therefore, the isolation and characterization of SP cells may provide novel insights for the development of alternative therapeutic agents to target cancer stem cells.
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Copy and paste a formatted citation
Spandidos Publications style
Wang M, Wang Y and Zhong J: Side population cells and drug resistance in breast cancer. Mol Med Rep 11: 4297-4302, 2015.
APA
Wang, M., Wang, Y., & Zhong, J. (2015). Side population cells and drug resistance in breast cancer. Molecular Medicine Reports, 11, 4297-4302. https://doi.org/10.3892/mmr.2015.3291
MLA
Wang, M., Wang, Y., Zhong, J."Side population cells and drug resistance in breast cancer". Molecular Medicine Reports 11.6 (2015): 4297-4302.
Chicago
Wang, M., Wang, Y., Zhong, J."Side population cells and drug resistance in breast cancer". Molecular Medicine Reports 11, no. 6 (2015): 4297-4302. https://doi.org/10.3892/mmr.2015.3291
Copy and paste a formatted citation
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Spandidos Publications style
Wang M, Wang Y and Zhong J: Side population cells and drug resistance in breast cancer. Mol Med Rep 11: 4297-4302, 2015.
APA
Wang, M., Wang, Y., & Zhong, J. (2015). Side population cells and drug resistance in breast cancer. Molecular Medicine Reports, 11, 4297-4302. https://doi.org/10.3892/mmr.2015.3291
MLA
Wang, M., Wang, Y., Zhong, J."Side population cells and drug resistance in breast cancer". Molecular Medicine Reports 11.6 (2015): 4297-4302.
Chicago
Wang, M., Wang, Y., Zhong, J."Side population cells and drug resistance in breast cancer". Molecular Medicine Reports 11, no. 6 (2015): 4297-4302. https://doi.org/10.3892/mmr.2015.3291
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