Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
June-2015 Volume 11 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
June-2015 Volume 11 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Effects of ethanol on the expression of caveolin-1 in HepG2 cells

  • Authors:
    • Ying Wang
    • Jing Tong
    • Bing Chang
    • Bai‑Fang Wang
    • Dai Zhang
    • Bing‑Yuan Wang
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterology, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China
  • Pages: 4409-4413
    |
    Published online on: February 4, 2015
       https://doi.org/10.3892/mmr.2015.3296
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

This study aimed to investigate the effects of ethanol on the expression of caveolin‑1 (CAV‑1) in HepG2 hepatocarcinoma cells. Ethanol‑treated HepG2 cells were investigated using the in vitro model to determine whether ethanol can influence the expression of CAV‑1. Cell viability was measured using the colorimetric 3‑(4, 5‑dimethylthiazol‑2‑yl)‑2,5‑diphenyltetrazolium bromide (MTT) and lactate dehydrogenase (LDH) assays. Expression of CAV‑1 was detected using western blot analysis. Quantitative PCR (qPCR) was used to determine CAV‑1 mRNA levels. The distribution of CAV‑1 in HepG2 cells was analyzed using immunofluorescence. The MTT assay results revealed that cell viability was not altered at ethanol concentrations of <1.0%, while ethanol concentrations >1.0% caused cell shedding, but not cell fragmentation. Western blot analysis showed significant differences in the levels of CAV‑1 expression between the control group and the 1.0% ethanol‑treated group at 6, 12 and 24 h (all P<0.05). qPCR showed significant differences in the expression levels of caveolin‑1 mRNA between the control group and the 1.0% ethanol‑treated group at 6 h, 12 h and 24 h (all P<0.05). Immunofluorescence demonstrated that CAV‑1 was distributed discontinuously at the boundaries of HepG2 cells. The results indicate that ethanol may increase the expression of CAV‑1 in HepG2 cells.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Fu Y, Hoang A, Escher G, et al: Expression of caveolin-1 enhances cholesterol efflux in hepatic cells. J Biol Chem. 279:14140–14146. 2004. View Article : Google Scholar : PubMed/NCBI

2 

Hendrickson DG, Hogan DJ, McCullough HL, et al: Concordant regulation of translation and mRNA abundance for hundreds of targets of a human microRNA. Plos Biol. 7:e10002382009. View Article : Google Scholar : PubMed/NCBI

3 

Yang XY, Huang CC, Kan QM, et al: Calcium regulates caveolin-1 expression at the transcriptional level. Biochem Biophys Res Commun. 426:334–341. 2012. View Article : Google Scholar : PubMed/NCBI

4 

Sotgia F, Martinez-Outschoorn UE, Howell A, et al: Caveolin-1 and cancer metabolism in the tumor microenvironment: markers, models, and mechanisms. Annu Rev Pathol. 7:423–467. 2012. View Article : Google Scholar

5 

Khraiwesh B, Arif MA, Seumel GI, et al: Transcriptional control of gene expression by microRNAs. Cell. 140:111–122. 2010. View Article : Google Scholar : PubMed/NCBI

6 

Sloan EK, Ciocca DR, Pouliot N, et al: Stromal cell expression of caveolin-1 predicts outcome in breast cancer. Am J Pathol. 174:2035–2043. 2009. View Article : Google Scholar : PubMed/NCBI

7 

Chen HL, Fan LF, Gao J, Ouyang JP and Zhang YX: Differential expression and function of the caveolin-1 gene in non-small cell lung carcinoma. Oncol Rep. 25:359–366. 2011. View Article : Google Scholar

8 

Thompson TC, Tahir SA, Li L, et al: The role of caveolin-1 in prostate cancer: clinical implications. Prostate Cancer Prostatic Dis. 13:6–11. 2010. View Article : Google Scholar :

9 

Yu S, Zhang L, Li N, et al: Caveolin-1 up-regulates ST6Gal-I to promote the adhesive capability of mouse hepatocarcinoma cells to fibronectin via FAK-mediated adhesion signaling. Biochem Biophys Res Commun. 427:506–512. 2012. View Article : Google Scholar : PubMed/NCBI

10 

Meyer C, Dzieran J, Liu Y, et al: Distinct dedifferentiation processes affect caveolin-1 expression in hepatocytes. Cell Commun Signal. 11:62013. View Article : Google Scholar : PubMed/NCBI

11 

Tse EY, Ko FC, Tung EK, et al: Caveolin-1 overexpression is associated with hepatocellular carcinoma tumourigenesis and metastasis. J Pathol. 226:645–653. 2012. View Article : Google Scholar

12 

Abraham J, Balbo S, Crabb D and Brooks PJ: Alcohol metabolism in human cells causes DNA damage and activates the Fanconi anemia-breast cancer susceptibility (FA-BRCA) DNA damage response network. Alcohol Clin Exp Res. 35:2113–2120. 2011. View Article : Google Scholar : PubMed/NCBI

13 

Seitz HK and Stickel F: Acetaldehyde as an underestimated risk factor for cancer development: role of genetics in ethanol metabolism. Genes Nutr. 5:121–128. 2010. View Article : Google Scholar :

14 

Cederbaum AI, Lu Y and Wu D: Role of oxidative stress in alcohol-induced liver injury. Arch Toxicol. 83:519–548. 2009. View Article : Google Scholar : PubMed/NCBI

15 

Vluggens A and Reddy JK: Nuclear receptors and transcription factors in the development of fatty liver disease. Curr Drug Metab. 13:1422–1435. 2012. View Article : Google Scholar : PubMed/NCBI

16 

Wang X and Abdel-Rahman AA: Effect of chronic ethanol administration on hepatic eNOS activity and its association with caveolin-1 and calmodulin in female rats. Am J Physiol Gastrointest Liver Physiol. 289:G579–G585. 2005. View Article : Google Scholar : PubMed/NCBI

17 

Denizot F and Lang R: Rapid colorimetric assay for cell growth and survival. Modifications to the tetrazolium dye procedure giving improved sensitivity and reliability. J Immunol Methods. 89:271–277. 1986. View Article : Google Scholar : PubMed/NCBI

18 

Vega-Avila E and Pugsley MK: An overview of colorimetric assay methods used to assess survival or proliferation of mammalian cells. Proc West Pharmacol Soc. 54:10–14. 2011.PubMed/NCBI

19 

Smith SM, Wunder MB, Norris DA and Shellman YG: A simple protocol for using a LDH-based cytotoxicity assay to assess the effects of death and growth inhibition at the same time. Plos One. 6:e269082011. View Article : Google Scholar : PubMed/NCBI

20 

Cummings BS, Wills LP and Schnellmann RG: Measurement of cell death in Mammalian cells. Curr Protoc Pharmacol. Chapter 12: Unit 12. pp. 82012, PubMed/NCBI

21 

Fuchs S, Hollins AJ, Laue M, et al: Differentiation of human alveolar epithelial cells in primary culture: morphological characterization and synthesis of caveolin-1 and surfactant protein-C. Cell Tissue Res. 311:31–45. 2003. View Article : Google Scholar

22 

Quest AF, Lobos-Gonzalez L, Nunez S, et al: The caveolin-1 connection to cell death and survival. Curr Mol Med. 13:266–281. 2013. View Article : Google Scholar

23 

Chidlow JH Jr and Sessa WC: Caveolae, caveolins, and cavins: complex control of cellular signalling and inflammation. Cardiovasc Res. 86:219–225. 2010. View Article : Google Scholar : PubMed/NCBI

24 

Garrean S, Gao XP, Brovkovych V, et al: Caveolin-1 regulates NF‑kappaB activation and lung inflammatory response to sepsis induced by lipopolysaccharide. J Immunol. 177:4853–4860. 2006. View Article : Google Scholar : PubMed/NCBI

25 

Razani B, Combs TP, Wang XB, et al: Caveolin‑1‑deficient mice are lean, resistant to diet-induced obesity, and show hypertriglyceridemia with adipocyte abnormalities. J Biol Chem. 277:8635–8647. 2002. View Article : Google Scholar

26 

Yokomori H, Oda M, Yoshimura K, et al: Vascular endothelial growth factor increases fenestral permeability in hepatic sinusoidal endothelial cells. Liver Int. 23:467–475. 2003. View Article : Google Scholar

27 

Giancotti FG: Complexity and specificity of integrin signalling. Nat Cell Biol. 2:E13–E14. 2000. View Article : Google Scholar : PubMed/NCBI

28 

Ten Dijke P, Goumans MJ, Itoh F and Itoh S: Regulation of cell proliferation by Smad proteins. J Cell Physiol. 191:1–16. 2002. View Article : Google Scholar : PubMed/NCBI

29 

Wang S, Kan Q, Sun Y, et al: Caveolin-1 regulates neural differentiation of rat bone mesenchymal stem cells into neurons by modulating Notch signaling. Int J Dev Neurosci. 31:30–35. 2013. View Article : Google Scholar

30 

Senetta R, Stella G, Pozzi E, et al: Caveolin-1 as a promoter of tumour spreading: when, how, where and why. J Cell Mol Med. 17:325–336. 2013. View Article : Google Scholar : PubMed/NCBI

31 

Tanase CP, Dima S, Mihai M, et al: Caveolin-1 overexpression correlates with tumour progression markers in pancreatic ductal adenocarcinoma. J Mol Histol. 40:23–29. 2009. View Article : Google Scholar : PubMed/NCBI

32 

Pancotti F, Roncuzzi L, Maggiolini M and Gasperi-Campani A: Caveolin-1 silencing arrests the proliferation of metastatic lung cancer cells through the inhibition of STAT3 signaling. Cell Signal. 24:1390–1397. 2012. View Article : Google Scholar : PubMed/NCBI

33 

Han F and Zhu HG: Caveolin-1 regulating the invasion and expression of matrix metalloproteinase (MMPs) in pancreatic carcinoma cells. J Surg Res. 159:443–450. 2010. View Article : Google Scholar

34 

Prinetti A, Aureli M, Illuzzi G, et al: GM3 synthase overexpression results in reduced cell motility and in caveolin-1 upregulation in human ovarian carcinoma cells. Glycobiology. 20:62–77. 2010. View Article : Google Scholar

35 

Yeh D, Chen C, Sun MZ, et al: Caveolin-1 is an important factor for the metastasis and proliferation of human small cell lung cancer NCI-H446 cell. Anat Rec (Hoboken). 292:1584–1592. 2009. View Article : Google Scholar

36 

Mastrodonato M, Portincasa P, Mentino D, et al: Caveolin-1 and mitochondrial alterations in regenerating rat liver. Microsc Res Tech. 75:1026–1032. 2012. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Wang Y, Tong J, Chang B, Wang BF, Zhang D and Wang BY: Effects of ethanol on the expression of caveolin-1 in HepG2 cells. Mol Med Rep 11: 4409-4413, 2015.
APA
Wang, Y., Tong, J., Chang, B., Wang, B., Zhang, D., & Wang, B. (2015). Effects of ethanol on the expression of caveolin-1 in HepG2 cells. Molecular Medicine Reports, 11, 4409-4413. https://doi.org/10.3892/mmr.2015.3296
MLA
Wang, Y., Tong, J., Chang, B., Wang, B., Zhang, D., Wang, B."Effects of ethanol on the expression of caveolin-1 in HepG2 cells". Molecular Medicine Reports 11.6 (2015): 4409-4413.
Chicago
Wang, Y., Tong, J., Chang, B., Wang, B., Zhang, D., Wang, B."Effects of ethanol on the expression of caveolin-1 in HepG2 cells". Molecular Medicine Reports 11, no. 6 (2015): 4409-4413. https://doi.org/10.3892/mmr.2015.3296
Copy and paste a formatted citation
x
Spandidos Publications style
Wang Y, Tong J, Chang B, Wang BF, Zhang D and Wang BY: Effects of ethanol on the expression of caveolin-1 in HepG2 cells. Mol Med Rep 11: 4409-4413, 2015.
APA
Wang, Y., Tong, J., Chang, B., Wang, B., Zhang, D., & Wang, B. (2015). Effects of ethanol on the expression of caveolin-1 in HepG2 cells. Molecular Medicine Reports, 11, 4409-4413. https://doi.org/10.3892/mmr.2015.3296
MLA
Wang, Y., Tong, J., Chang, B., Wang, B., Zhang, D., Wang, B."Effects of ethanol on the expression of caveolin-1 in HepG2 cells". Molecular Medicine Reports 11.6 (2015): 4409-4413.
Chicago
Wang, Y., Tong, J., Chang, B., Wang, B., Zhang, D., Wang, B."Effects of ethanol on the expression of caveolin-1 in HepG2 cells". Molecular Medicine Reports 11, no. 6 (2015): 4409-4413. https://doi.org/10.3892/mmr.2015.3296
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team