Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
June-2015 Volume 11 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
June-2015 Volume 11 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Apoptosis of Bel-7402 human hepatoma cells induced by a ruthenium(II) complex coordinated by cordycepin through the p53 pathway

  • Authors:
    • Qun Lu
    • Wenjie Mei
    • Shaohong Luo
    • Weibin He
  • View Affiliations / Copyright

    Affiliations: Department of Biochemical and Molecular Biology, School of Basic Courses, Guangdong Pharmaceutical University, Guangzhou, Guangdong 510006, P.R. China
  • Pages: 4424-4430
    |
    Published online on: February 4, 2015
       https://doi.org/10.3892/mmr.2015.3297
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

A ruthenium(II) complex coordinated by cordycepin, [Cor‑Ru(II)], was synthesized for investigation as a potential novel candidate for cancer therapy. The antitumor activity of Cor‑Ru(II) was investigated by MTT and flow cytometry (FCM) assays. The results showed that Cor‑Ru(II) significantly inhibited the proliferation of Bel‑7402 human hepatoma cells and delayed cell cycle progression. Subsequent experiments using FCM and western blot analysis indicated that Cor‑Ru(II) induced cell apoptosis via suppression of bcl‑2 expression and stimulation of p53 expression. Cor‑Ru(II) was shown to interfere with the synthesis of DNA. This was confirmed by the in vitro binding to DNA in the groove binding mode (Kb=4.22(±0.2)x105 M‑1). Thus, Cor‑Ru(II) appears to act as an effective antitumor agent in vitro.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

View References

1 

Xu L, Liu Y, Tan YH and Mei WJ: The synthesis of a novel ruthenium (II) complex with cordycepin and preliminary research of its killing action on rat liver cancer cells. J Guangzhou Univ Trad Chin Med. 25:215–219. 2008.In Chinese.

2 

Mei WJ, Liu Y, Liu J, Li J, Zheng K and Ji L: Synthesis, characterization and DNA-binding properties of mixed porphyrin-polypyridyl ruthenium(II) complexes. Trans Met Chem. 30:82–88. 2005. View Article : Google Scholar

3 

Mei WJ, Liu YJ, Wang N, Ma YZ, Wang H, Luo LQ and Huang SL: The photocleavage properties of a novel ruthenium(II) complex on liver cancer cells Bel-7402 DNA. Trans Met Chem. 31:1024–1027. 2006. View Article : Google Scholar

4 

Sava G, Zorzet S, Giraldi T, Mestroni G and Zassinovich G: Antineoplastic activity and toxicity of an organometallic complex of ruthenium(II) in comparison with cis-PDD in mice bearing solid malignant neoplasms. Eur J Cancer Clin Oncol. 20:841–847. 1984. View Article : Google Scholar : PubMed/NCBI

5 

Keppler BK, Henn M, Juhl UM, Berger MR, Niebl R and Wagner FE: New ruthenium complexes for the treatment of cancer. Progress in Clinical Biochemistry and Medicine. 10. Springer-Verlag Berlin Heidelberg; Germany: pp. 41–69. 1989, View Article : Google Scholar

6 

Sava G, Bergamo A, Zorzet S, et al: Influence of chemical stability on the activity of the antimetastasis ruthenium compound NAMI-A. Eur J Cancer. 38:427–435. 2002. View Article : Google Scholar : PubMed/NCBI

7 

Hotze AC, Velders AH, Ugozzoli F, et al: Synthesis, characterization, and crystal structure of alpha-[Ru(azpy)2(NO3)2] (azpy = 2-(phenylazo)pyridine) and the products of its reactions with guanine derivatives. Inorg Chem. 39:3838–3844. 2000. View Article : Google Scholar

8 

Zhang QL, Liu JG, Liu J, et al: DNA-binding and photo-cleavage studies of cobalt(III) mixed-polypyridyl complexes containing 2-(2-chloro-5-nitrophenyl)imidazo [4,5-f][1,10] phenanthroline. J Inorg Biochem. 85:291–296. 2001. View Article : Google Scholar : PubMed/NCBI

9 

Zou XH, Ye BH, Li H, et al: The design of new molecular ‘light switches’ for DNA. J Biol Inorg Chem. 6:143–150. 2001. View Article : Google Scholar : PubMed/NCBI

10 

Shu RH, Cai XD, Tan JP, Wei JY, Jiang Y and Wu FF: Effects of baicalin zinc complex on the immune responses in mice. Railway Med J. 17:321–323. 1989.In Chinese.

11 

Jiang Y: The observation of influence of baicalin Zinc complex in guinea pigs sensibilized on releasing SRS-A from its isolated lung. Railway Med J. 15:290–292. 1987.In Chinese.

12 

Chen ZF, Zhang M, Shi SM, et al: Synthesis, crystal structure and antibacterial activity of 2-aminobenzothiazole silver(I) nitrate. Chin J Appl Chem. 22:730–733. 2005.In Chinese.

13 

Yoshida J, Takamura S, Yamaguchi N, et al: Antitumor activity of an extract of Cordyceps sinensis (Berk) Sacc against murine tumor cell lines. Jpn J Exp Med. 59:157–161. 1989.PubMed/NCBI

14 

Thomadaki H, Scorilas A, Tsiapalis CM and Havredaki M: The role of cordycepin in cancer treatment via induction or inhibition of apoptosis: implication of polyadenylation in a cell type specific manner. Cancer Chemother Pharmacol. 61:251–265. 2008. View Article : Google Scholar

15 

Kodama EN, McCaffrey RP, Yusa K and Mitsuya H: Antileukemic activity and mechanism of action of cordycepin against terminal deoxynucleotidal transferase-positive (TdT+) leukemic cells. Biochem Pharmacol. 59:273–281. 2000. View Article : Google Scholar

16 

Lu Q, Qiu TQ and Yang H: Ligustilide inhibits vascular smooth muscle cells proliferation. Eur J Pharmacol. 542:136–140. 2006. View Article : Google Scholar : PubMed/NCBI

17 

Ku HK, Lim HM, Oh KH, Yang HJ, Jeong JS and Kim SK: Interpretation of protein quantitation using the Bradford assay: comparison with two calculation models. Anal Biochem. 434:178–180. 2013. View Article : Google Scholar

18 

Maheswafi PU and Palaniandavar M: DNA binding and cleavage activity Ru(Nti3)4(di-imine)]C12complexes. Inorg Chim Acta. 357:901–912. 2004. View Article : Google Scholar

19 

Hartshorn RM and Barton JK: Novel dipyfidophenazine complexes of ruthenium(II): exploring luminescent reporters of DNA. J Am Chem Soc. 114:5919–5925. 1992. View Article : Google Scholar

20 

Neyhart GA, Grover N, Smith SR, et al: Binding and kinetics studies of oxidation of DNA by oxoruthenium(IV). J Am Chem Soc. 115:4423–4428. 1993. View Article : Google Scholar

21 

Nakabayashi Y, Iwamoto N, Inada H and Yamauchi O: DNA-binding properties of flexible diamine bridged dinuclear ruthenium(II)-2,2′-bipyridine complexes. Inorg Chem Commun. 9:1033–1036. 2006. View Article : Google Scholar

22 

Hong J, Yang GS, Duan CY, Guo ZJ and Zhu LG: Fluorescence quenching of EB-DNA complex by a novel di-bipyridyl ruthenium(II) complex of p-tert-butyltetrathiacalix[4]arene. Inorg Chem Commun. 8:988–991. 2005. View Article : Google Scholar

23 

El-Serag HB: Hepatocellular carcinoma. N Eng J Med. 365:1118–1127. 2011. View Article : Google Scholar

24 

Alenzi FQ: Links between apoptosis, proliferation and the cell cycle. Br J Biomed Sci. 61:99–102. 2004.PubMed/NCBI

25 

Vaseva AV and Moll UM: The mitochondrial p53 pathway. Biochim Biophys Acta. 1787:414–420. 2009. View Article : Google Scholar

26 

Korsmeyer SJ, Shutter JR, Veis DJ, Merry DE and Oltvai ZN: Bcl-2/Bax: a rheostat that regulates an anti-oxidant pathway and cell death. Semin Cancer Biol. 4:327–332. 1993.PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Lu Q, Mei W, Luo S and He W: Apoptosis of Bel-7402 human hepatoma cells induced by a ruthenium(II) complex coordinated by cordycepin through the p53 pathway. Mol Med Rep 11: 4424-4430, 2015.
APA
Lu, Q., Mei, W., Luo, S., & He, W. (2015). Apoptosis of Bel-7402 human hepatoma cells induced by a ruthenium(II) complex coordinated by cordycepin through the p53 pathway. Molecular Medicine Reports, 11, 4424-4430. https://doi.org/10.3892/mmr.2015.3297
MLA
Lu, Q., Mei, W., Luo, S., He, W."Apoptosis of Bel-7402 human hepatoma cells induced by a ruthenium(II) complex coordinated by cordycepin through the p53 pathway". Molecular Medicine Reports 11.6 (2015): 4424-4430.
Chicago
Lu, Q., Mei, W., Luo, S., He, W."Apoptosis of Bel-7402 human hepatoma cells induced by a ruthenium(II) complex coordinated by cordycepin through the p53 pathway". Molecular Medicine Reports 11, no. 6 (2015): 4424-4430. https://doi.org/10.3892/mmr.2015.3297
Copy and paste a formatted citation
x
Spandidos Publications style
Lu Q, Mei W, Luo S and He W: Apoptosis of Bel-7402 human hepatoma cells induced by a ruthenium(II) complex coordinated by cordycepin through the p53 pathway. Mol Med Rep 11: 4424-4430, 2015.
APA
Lu, Q., Mei, W., Luo, S., & He, W. (2015). Apoptosis of Bel-7402 human hepatoma cells induced by a ruthenium(II) complex coordinated by cordycepin through the p53 pathway. Molecular Medicine Reports, 11, 4424-4430. https://doi.org/10.3892/mmr.2015.3297
MLA
Lu, Q., Mei, W., Luo, S., He, W."Apoptosis of Bel-7402 human hepatoma cells induced by a ruthenium(II) complex coordinated by cordycepin through the p53 pathway". Molecular Medicine Reports 11.6 (2015): 4424-4430.
Chicago
Lu, Q., Mei, W., Luo, S., He, W."Apoptosis of Bel-7402 human hepatoma cells induced by a ruthenium(II) complex coordinated by cordycepin through the p53 pathway". Molecular Medicine Reports 11, no. 6 (2015): 4424-4430. https://doi.org/10.3892/mmr.2015.3297
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team