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Article

Chloroform fraction of Euphorbia maculata has antiplatelet activity via suppressing thromboxane B2 formation

  • Authors:
    • Se‑Uk Kwon
    • Ji‑Yoon Cha
    • Hoon‑Yeon Lee
    • Mingjie Xin
    • Su‑Jeong Ji
    • Dae‑Ki Kim
    • Do‑Sim Park
    • Mi‑Kyung Pyo
    • Young‑Mi Lee
  • View Affiliations / Copyright

    Affiliations: Department of Oriental Pharmacy, College of Pharmacy, Wonkwang‑Oriental Medicines Research Institute, Wonkwang University, Iksan, Jeonbuk 570‑749, Republic of Korea, Department of Food Industry Convergence, Wonkwang University, Iksan, Jeonbuk 570‑749, Republic of Korea, Department of Immunology and Institute of Medical Science, Jeonbuk National University Medical School, Jeonju, Jeonbuk 561‑180, Republic of Korea, Department of Laboratory Medicine, School of Medicine, Wonkwang University, Iksan, Jeonbuk 570‑749, Republic of Korea, International Ginseng and Herb Research Institute, Guemsan, Chungbuk 312‑804, Republic of Korea
  • Pages: 4255-4261
    |
    Published online on: February 9, 2015
       https://doi.org/10.3892/mmr.2015.3319
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Abstract

Euphorbia maculata (EM) is a traditionally used antidiarrheal, antibacterial, antifungal and antioxidant agent. However, the effects of EM on platelet activity remain to be elucidated. Therefore, the present study investigated the antiplatelet effect of various EM extract fractions on platelet aggregation in rats. The antiplatelet activity of the EM fractions on collagen or adenosine diphosphate (ADP)‑induced platelet aggregation was evaluated in vitro and ex vivo. Thromboxane B2 (TXB2) formation, rat‑tail bleeding time and coagulation time were also measured. Among the fractions, the chloroform fraction of EM (CFEM) significantly inhibited ADP‑induced platelet aggregation in vitro. Furthermore, oral administration of 50 mg/kg CFEM to rats significantly reduced ADP‑induced platelet aggregation without increasing the tail bleeding time or coagulation time. In addition, EM significantly inhibited the level of TXB2 formation in a dose‑dependent manner. These results suggest that CFEM exhibits antiplatelet activity, without causing bleeding, via the suppression of TXB2 formation. CFEM may be a type of food which has the potential for preventing cardiovascular disease.
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Copy and paste a formatted citation
Spandidos Publications style
Kwon SU, Cha JY, Lee HY, Xin M, Ji SJ, Kim DK, Park DS, Pyo MK and Lee YM: Chloroform fraction of Euphorbia maculata has antiplatelet activity via suppressing thromboxane B2 formation. Mol Med Rep 11: 4255-4261, 2015.
APA
Kwon, S., Cha, J., Lee, H., Xin, M., Ji, S., Kim, D. ... Lee, Y. (2015). Chloroform fraction of Euphorbia maculata has antiplatelet activity via suppressing thromboxane B2 formation. Molecular Medicine Reports, 11, 4255-4261. https://doi.org/10.3892/mmr.2015.3319
MLA
Kwon, S., Cha, J., Lee, H., Xin, M., Ji, S., Kim, D., Park, D., Pyo, M., Lee, Y."Chloroform fraction of Euphorbia maculata has antiplatelet activity via suppressing thromboxane B2 formation". Molecular Medicine Reports 11.6 (2015): 4255-4261.
Chicago
Kwon, S., Cha, J., Lee, H., Xin, M., Ji, S., Kim, D., Park, D., Pyo, M., Lee, Y."Chloroform fraction of Euphorbia maculata has antiplatelet activity via suppressing thromboxane B2 formation". Molecular Medicine Reports 11, no. 6 (2015): 4255-4261. https://doi.org/10.3892/mmr.2015.3319
Copy and paste a formatted citation
x
Spandidos Publications style
Kwon SU, Cha JY, Lee HY, Xin M, Ji SJ, Kim DK, Park DS, Pyo MK and Lee YM: Chloroform fraction of Euphorbia maculata has antiplatelet activity via suppressing thromboxane B2 formation. Mol Med Rep 11: 4255-4261, 2015.
APA
Kwon, S., Cha, J., Lee, H., Xin, M., Ji, S., Kim, D. ... Lee, Y. (2015). Chloroform fraction of Euphorbia maculata has antiplatelet activity via suppressing thromboxane B2 formation. Molecular Medicine Reports, 11, 4255-4261. https://doi.org/10.3892/mmr.2015.3319
MLA
Kwon, S., Cha, J., Lee, H., Xin, M., Ji, S., Kim, D., Park, D., Pyo, M., Lee, Y."Chloroform fraction of Euphorbia maculata has antiplatelet activity via suppressing thromboxane B2 formation". Molecular Medicine Reports 11.6 (2015): 4255-4261.
Chicago
Kwon, S., Cha, J., Lee, H., Xin, M., Ji, S., Kim, D., Park, D., Pyo, M., Lee, Y."Chloroform fraction of Euphorbia maculata has antiplatelet activity via suppressing thromboxane B2 formation". Molecular Medicine Reports 11, no. 6 (2015): 4255-4261. https://doi.org/10.3892/mmr.2015.3319
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