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Analysis of mechanical ventilation and lipopolysaccharide‑induced acute lung injury using DNA microarray analysis

  • Authors:
    • Yuqing Chen
    • Xin Zhou
    • Ling Rong
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    Affiliations: Department of Respiratory Medicine, Shanghai First People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200080, P.R. China, Department of Respiratory Medicine, The People's Hospital of Bozhou, Bozhou, Anhui 236804, P.R. China
    Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 4239-4245
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    Published online on: February 11, 2015
       https://doi.org/10.3892/mmr.2015.3335
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Abstract

Gene expression profiles of samples taken from patients with acute lung injury (ALI) induced by mechanical ventilation (MV) and lipopolysaccharide (LPS) were analyzed in order to identify key genes, and explore the underlying mechanisms. The GSE2411 microarray data set was downloaded from the Gene Expression Omnibus. This data set contained microarray data from 24 mouse lung samples, which were equally divided into four groups: Control group, MV group, LPS group and MV+LPS group. Differentially expressed genes (DEGs) were identified in the MV, LPS and MV+LPS groups, as compared with the control group, using packages of R software. Hierarchical clustering and between‑group comparisons were performed for each group of DEGs. Overrepresented biological processes were revealed by functional enrichment analysis using the Database for Annotation, Visualization and Integrated Discovery. Unique DEGs in the LPS and MV+LPS groups were selected, and pathway enrichment analyses were performed using the Kyoto Encyclopedia of Genes and Genomes Orthology Based Annotation system. A total of 32, 264 and 685 DEGs were identified in the MV, LPS and MV+LPS groups, respectively. The MV+LPS group had more DEGs, as compared with the other two treatment groups. Genes associated with the immune and inflammatory responses were significantly overrepresented in both the LPS and MV+LPS groups, suggesting that LPS dominated the progression of ALI. Unique DEGs in the LPS and MV+LPS groups were associated with cytokine‑cytokine receptor interaction. The Janus kinase‑signal transducer and activator of transcription signaling pathway was shown to be enriched in the LPS+MV‑unique DEGs. The results of the present study demonstrated that MV could exaggerate the transcriptional response of the lungs to LPS. Numerous key genes were identified, which may advance knowledge regarding the pathogenesis of ALI.
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Copy and paste a formatted citation
Spandidos Publications style
Chen Y, Zhou X and Rong L: Analysis of mechanical ventilation and lipopolysaccharide‑induced acute lung injury using DNA microarray analysis. Mol Med Rep 11: 4239-4245, 2015.
APA
Chen, Y., Zhou, X., & Rong, L. (2015). Analysis of mechanical ventilation and lipopolysaccharide‑induced acute lung injury using DNA microarray analysis. Molecular Medicine Reports, 11, 4239-4245. https://doi.org/10.3892/mmr.2015.3335
MLA
Chen, Y., Zhou, X., Rong, L."Analysis of mechanical ventilation and lipopolysaccharide‑induced acute lung injury using DNA microarray analysis". Molecular Medicine Reports 11.6 (2015): 4239-4245.
Chicago
Chen, Y., Zhou, X., Rong, L."Analysis of mechanical ventilation and lipopolysaccharide‑induced acute lung injury using DNA microarray analysis". Molecular Medicine Reports 11, no. 6 (2015): 4239-4245. https://doi.org/10.3892/mmr.2015.3335
Copy and paste a formatted citation
x
Spandidos Publications style
Chen Y, Zhou X and Rong L: Analysis of mechanical ventilation and lipopolysaccharide‑induced acute lung injury using DNA microarray analysis. Mol Med Rep 11: 4239-4245, 2015.
APA
Chen, Y., Zhou, X., & Rong, L. (2015). Analysis of mechanical ventilation and lipopolysaccharide‑induced acute lung injury using DNA microarray analysis. Molecular Medicine Reports, 11, 4239-4245. https://doi.org/10.3892/mmr.2015.3335
MLA
Chen, Y., Zhou, X., Rong, L."Analysis of mechanical ventilation and lipopolysaccharide‑induced acute lung injury using DNA microarray analysis". Molecular Medicine Reports 11.6 (2015): 4239-4245.
Chicago
Chen, Y., Zhou, X., Rong, L."Analysis of mechanical ventilation and lipopolysaccharide‑induced acute lung injury using DNA microarray analysis". Molecular Medicine Reports 11, no. 6 (2015): 4239-4245. https://doi.org/10.3892/mmr.2015.3335
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