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Article

Downregulation of the expression of B‑cell lymphoma‑extra large by RNA interference induces apoptosis and enhances the radiosensitivity of non‑small cell lung cancer cells

  • Authors:
    • Changbin Yang
    • Wei Huang
    • Ling Yan
    • Yu Wang
    • Weili Wang
    • Dezhi Liu
    • Xiaojun Zuo
  • View Affiliations / Copyright

    Affiliations: Department of Radiation Oncology, The Tumor Hospital of Jilin Province, Changchun, Jilin 130012, P.R. China, Department of Nuclear Medicine, The Tumor Hospital of Jilin Province, Changchun, Jilin 130012, P.R. China
  • Pages: 449-455
    |
    Published online on: February 12, 2015
       https://doi.org/10.3892/mmr.2015.3346
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Abstract

B‑cell lymphoma‑extra large (Bcl‑xL), an important member of anti‑apoptotic Bcl‑2 family, is involved in tumor progression and development. The overexpression of Bcl‑xL is associated with radioresistance of human malignancies. The present study aimed to investigate the inhibitory effect of small interfering RNA (siRNA) on the expression of Bcl‑xL in the A549 non‑small lung cancer (NSCLC) cell line, and its role in inducing the apoptosis and increasing the radiosensitivity of A549 cells. An siRNA expression vector, pSilencer4‑CMVneo‑short hairpin (sh)RNA, was constructed and stably transfected into A549 cells. The effects of Bcl‑xL‑shRNA on cell proliferation, apoptosis and the protein expression levels of associated proteins were assessed in vitro in the A549 cells. The radiosensitivity of the A549 cells was evaluated using a clonogenic cell survival assay. The results demonstrated that the sequence‑specific siRNA targeting Bcl‑xL efficiently and specifically downregulated the mRNA and protein expression levels of Bcl‑xL. The RNA interference‑mediated downregulation in the expression of Bcl‑xL inhibited cell proliferation, induced apoptosis and reduced the radioresistance of the NSCLC cells. These findings suggested that Bcl‑xL may be a promising therapeutic approach for the treatment of NSCLC.
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1 

Jemal A, Bray F, Center MM, Ferlay J, Ward E and Forman D: Global cancer statistics. CA Cancer J Clin. 61:69–90. 2011. View Article : Google Scholar : PubMed/NCBI

2 

Nygren P and Glimelius B; SBU-group: Swedish Council on Technology Assessment in Health Care: The Swedish Council on Technology Assessment in Health Care (SBU) report on Cancer Chemotherapy - Project objectives, the working process, key definitions and general aspects on cancer trial methodology and interpretation. Acta Oncol. 40:155–165. 2001. View Article : Google Scholar

3 

Impicciatore G, Sancilio S, Miscia S and Di Pietro R: Nutlins and ionizing radiation in cancer therapy. Curr Pharm Des. 16:1427–1442. 2010. View Article : Google Scholar : PubMed/NCBI

4 

Zhang S, Wang L, Liu H, Zhao G and Ming L: Enhancement of recombinant myricetin on the radiosensitivity of lung cancer A549 and H1299 cells. Diagn Pathol. 9:682014. View Article : Google Scholar : PubMed/NCBI

5 

Dumont F, Altmeyer A and Bischoff P: Radiosensitising agents for the radiotherapy of cancer: novel molecularly targeted approaches. Expert Opin Ther Pat. 19:775–799. 2009. View Article : Google Scholar : PubMed/NCBI

6 

Bischoff P, Altmeyer A and Dumont F: Radiosensitising agents for the radiotherapy of cancer: advances in traditional and hypoxia targeted radiosensitisers. Expert Opin Ther Pat. 19:643–662. 2009. View Article : Google Scholar : PubMed/NCBI

7 

Adams JM and Cory S: The Bcl-2 protein family: arbiters of cell survival. Science. 281:1322–1326. 1998. View Article : Google Scholar : PubMed/NCBI

8 

Walensky LD: BCL-2 in the crosshairs: tipping the balance of life and death. Cell Death Differ. 13:1339–1350. 2006. View Article : Google Scholar : PubMed/NCBI

9 

Yang TM, Barbone D, Fennell DA and Broaddus VC: Bcl-2 family proteins contribute to apoptotic resistance in lung cancer multi-cellular spheroids. Am J Respir Cell Mol Biol. 41:14–23. 2009. View Article : Google Scholar :

10 

Yip KW and Reed JC: Bcl-2 family proteins and cancer. Oncogene. 27:6398–6406. 2008. View Article : Google Scholar : PubMed/NCBI

11 

Kirkin V, Joos S and Zörnig M: The role of Bcl-2 family members in tumorigenesis. Biochim Biophys Acta. 1644:229–249. 2004. View Article : Google Scholar : PubMed/NCBI

12 

Soini Y, Kinnula V, Kaarteenaho-Wiik R, Kurttila E, Linnainmaa K and Pääkko P: Apoptosis and expression of apoptosis regulating proteins bcl-2, mcl-1, bcl-X, and bax in malignant mesothelioma. Clin Cancer Res. 5:3508–3515. 1999.PubMed/NCBI

13 

Karczmarek-Borowska B, Filip A, Wojcierowski J, et al: Estimation of prognostic value of Bcl-xL gene expression in non-small cell lung cancer. Lung Cancer. 51:61–69. 2006. View Article : Google Scholar

14 

Gottlieb E, Vander Heiden MG and Thompson CB: Bcl-x(L) prevents the initial decrease in mitochondrial membrane potential and subsequent reactive oxygen species production during tumor necrosis factor alpha-induced apoptosis. Mol Cell Biol. 20:5680–5689. 2000. View Article : Google Scholar : PubMed/NCBI

15 

Guichard SM, Hua ML, Kang P, Macpherson JS and Jodrell DI: Short hairpin RNAs targeting Bcl-xL modulate senescence and apoptosis following SN-38 and irinotecan exposure in a colon cancer model. Cancer Chemother Pharmacol. 60:651–660. 2007. View Article : Google Scholar : PubMed/NCBI

16 

Zhu H, Guo W, Zhang L, et al: Bcl-xL small interfering RNA suppresses the proliferation of 5-fluorouracil-resistant human colon cancer cells. Mol Cancer Ther. 4:451–456. 2005.PubMed/NCBI

17 

Nita ME, Ono-Nita SK, Tsuno N, et al: Bcl-X(L) antisense sensitizes human colon cancer cell line to 5-fluorouracil. Jpn J Cancer Res. 91:825–832. 2000. View Article : Google Scholar : PubMed/NCBI

18 

Varin E, Denoyelle C, Brotin E, et al: Downregulation of Bcl-xL and Mcl-1 is sufficient to induce cell death in mesothelioma cells highly refractory to conventional chemotherapy. Carcinogenesis. 31:984–993. 2010. View Article : Google Scholar : PubMed/NCBI

19 

Lei X, Huang Z, Zhong M, Zhu B, Tang S and Liao D: Bcl-xL small interfering RNA sensitizes cisplatin-resistant human lung adenocarcinoma cells. Acta Biochim Biophys Sin (Shanghai). 39:344–350. 2007. View Article : Google Scholar

20 

Yang J, Sun M, Zhang A, Lv C, De W and Wang Z: Adenovirus-mediated siRNA targeting Bcl-xL inhibits proliferation, reduces invasion and enhances radiosensitivity of human colorectal cancer cells. World J Surg Oncol. 9:1172011. View Article : Google Scholar : PubMed/NCBI

21 

Uprichard SL: The therapeutic potential of RNA interference. FEBS Lett. 579:5996–6007. 2005. View Article : Google Scholar : PubMed/NCBI

22 

Sui G, Soohoo C, Affar el B, et al: A DNA vector-based RNAi technology to suppress gene expression in mammalian cells. Proc Natl Acad Sci USA. 99:5515–5520. 2002. View Article : Google Scholar : PubMed/NCBI

23 

Pfaffl MW: A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res. 29:e452001. View Article : Google Scholar : PubMed/NCBI

24 

Chetty C, Bhoopathi P, Rao JS and Lakka SS: Inhibition of matrix metalloproteinase-2 enhances radiosensitivity by abrogating radiation-induced FoxM1-mediated G2/M arrest in A549 lung cancer cells. Int J Cancer. 124:2468–2477. 2009. View Article : Google Scholar : PubMed/NCBI

25 

Baumann M, Stamatis G and Thomas M: Therapy of localized non-small cell lung cancer (take home messages). Lung Cancer. 33(Suppl 1): S47–S49. 2001. View Article : Google Scholar : PubMed/NCBI

26 

Duchesne GM: Fundamental bases of combined therapy in lung cancer: cell resistance to chemotherapy and radiotherapy. Lung Cancer. 10(Suppl 1): S67–S72. 1994. View Article : Google Scholar : PubMed/NCBI

27 

Gressen EL and Curran WJ: Hyperfractionated radiotherapy for lung cancer. Curr Oncol Rep. 2:71–75. 2000. View Article : Google Scholar : PubMed/NCBI

28 

Llambi F and Green DR: Apoptosis and oncogenesis: give and take in the BCL-2 family. Curr Opin Genet Dev. 21:12–20. 2011. View Article : Google Scholar : PubMed/NCBI

29 

Streffer JR, Rimner A, Rieger J, Naumann U, Rodemann HP and Weller M: BCL-2 family proteins modulate radiosensitivity in human malignant glioma cells. J Neurooncol. 56:43–49. 2002. View Article : Google Scholar : PubMed/NCBI

30 

Masui T, Hosotani R, Ito D, et al: Bcl-xL antisense oligonucleotides coupled with antennapedia enhances radiation-induced apoptosis in pancreatic cancer. Surgery. 140:149–160. 2006. View Article : Google Scholar : PubMed/NCBI

31 

Wang R, Lin F, Wang X, et al: Suppression of Bcl-xL expression by a novel tumor-specific RNA interference system inhibits proliferation and enhances radiosensitivity in prostatic carcinoma cells. Cancer Chemother Pharmacol. 61:943–952. 2008. View Article : Google Scholar

32 

Lei XY, Zhong M, Feng LF, Zhu BY, Tang SS and Liao DF: Bcl-xL small interfering RNA enhances sensitivity of Hepg2 hepatocellular carcinoma cells to 5-fluorouracil and hydroxycamptothecin. Acta Biochim Biophys Sin (Shanghai). 38:704–710. 2006. View Article : Google Scholar

33 

Zhang J and Hua ZC: Targeted gene silencing by small interfering RNA-based knock-down technology. Curr Pharm Biotechnol. 5:1–7. 2004. View Article : Google Scholar : PubMed/NCBI

34 

Matsuyama Y, Yamayoshi A, Kobori A and Murakami A: Functional regulation of RNA-induced silencing complex by photoreactive oligonucleotides. Bioorg Med Chem. 22:1003–1007. 2014. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Yang C, Huang W, Yan L, Wang Y, Wang W, Liu D and Zuo X: Downregulation of the expression of B‑cell lymphoma‑extra large by RNA interference induces apoptosis and enhances the radiosensitivity of non‑small cell lung cancer cells. Mol Med Rep 12: 449-455, 2015.
APA
Yang, C., Huang, W., Yan, L., Wang, Y., Wang, W., Liu, D., & Zuo, X. (2015). Downregulation of the expression of B‑cell lymphoma‑extra large by RNA interference induces apoptosis and enhances the radiosensitivity of non‑small cell lung cancer cells. Molecular Medicine Reports, 12, 449-455. https://doi.org/10.3892/mmr.2015.3346
MLA
Yang, C., Huang, W., Yan, L., Wang, Y., Wang, W., Liu, D., Zuo, X."Downregulation of the expression of B‑cell lymphoma‑extra large by RNA interference induces apoptosis and enhances the radiosensitivity of non‑small cell lung cancer cells". Molecular Medicine Reports 12.1 (2015): 449-455.
Chicago
Yang, C., Huang, W., Yan, L., Wang, Y., Wang, W., Liu, D., Zuo, X."Downregulation of the expression of B‑cell lymphoma‑extra large by RNA interference induces apoptosis and enhances the radiosensitivity of non‑small cell lung cancer cells". Molecular Medicine Reports 12, no. 1 (2015): 449-455. https://doi.org/10.3892/mmr.2015.3346
Copy and paste a formatted citation
x
Spandidos Publications style
Yang C, Huang W, Yan L, Wang Y, Wang W, Liu D and Zuo X: Downregulation of the expression of B‑cell lymphoma‑extra large by RNA interference induces apoptosis and enhances the radiosensitivity of non‑small cell lung cancer cells. Mol Med Rep 12: 449-455, 2015.
APA
Yang, C., Huang, W., Yan, L., Wang, Y., Wang, W., Liu, D., & Zuo, X. (2015). Downregulation of the expression of B‑cell lymphoma‑extra large by RNA interference induces apoptosis and enhances the radiosensitivity of non‑small cell lung cancer cells. Molecular Medicine Reports, 12, 449-455. https://doi.org/10.3892/mmr.2015.3346
MLA
Yang, C., Huang, W., Yan, L., Wang, Y., Wang, W., Liu, D., Zuo, X."Downregulation of the expression of B‑cell lymphoma‑extra large by RNA interference induces apoptosis and enhances the radiosensitivity of non‑small cell lung cancer cells". Molecular Medicine Reports 12.1 (2015): 449-455.
Chicago
Yang, C., Huang, W., Yan, L., Wang, Y., Wang, W., Liu, D., Zuo, X."Downregulation of the expression of B‑cell lymphoma‑extra large by RNA interference induces apoptosis and enhances the radiosensitivity of non‑small cell lung cancer cells". Molecular Medicine Reports 12, no. 1 (2015): 449-455. https://doi.org/10.3892/mmr.2015.3346
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