α-lipoic acid protects against hypoxia/reoxygenation-induced injury in human umbilical vein endothelial cells through suppression of apoptosis and autophagy

Zhang,Jingjing; Deng,Houliang; Liu,Li; Liu,Xiaoxia; Zuo,Xialin; Xu,Qian; Wu,Zhuomin; Peng,Xiaobin; Ji,Aimin

doi:10.3892/mmr.2015.3351

July-2015 Volume 12 Issue 1

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July-2015 Volume 12 Issue 1

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Article Open Access

α-lipoic acid protects against hypoxia/reoxygenation-induced injury in human umbilical vein endothelial cells through suppression of apoptosis and autophagy

Authors:
- Jingjing Zhang
- Houliang Deng
- Li Liu
- Xiaoxia Liu
- Xialin Zuo
- Qian Xu
- Zhuomin Wu
- Xiaobin Peng
- Aimin Ji
View Affiliations / Copyright

Affiliations: Center for Drug Research and Development, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong 510282, P.R. China , Institute of Neurosciences, The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong 510260, P.R. China, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China

Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
Pages: 180-186
|
Published online on: February 13, 2015

https://doi.org/10.3892/mmr.2015.3351
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Abstract

α‑lipoic acid (ALA) is known as a powerful antioxidant, which has been reported to have protective effects against various cardiovascular diseases. The present study aimed to determine whether ALA pre‑ or post‑treatment induced protective effects against hypoxia/reoxygenation‑induced injury via inhibition of apoptosis and autophagy in human umbilical vein endothelial cells (HUVECs). In order to simulate the conditions of hypoxia/reoxygenation, HUVECs were subjected to 4 h of oxygen‑glucose deprivation (OGD) followed by 12 h of reoxygenation. For the pre‑treatment, ALA was added to the buffer 12 h prior to OGD, whereas for the post‑treatment, ALA was added at the initiation of reoxygenation. The results demonstrated that ALA pre‑ or post‑treatment significantly reduced lactate dehydrogenase (LDH) release induced through hypoxia/reoxygenation in HUVECs in a dose‑dependent manner; of note, 1 mM ALA pre‑ or post‑treatment exhibited the most potent protective effects. In addition, ALA significantly reduced hypoxia/reoxygenation‑induced loss of mitochondrial membrane potential, apoptosis and the expression of cleaved caspase‑3 in HUVECs. In the presence of the specific autophagy inhibitor 3‑methyladenine, hypoxia/reoxygenation‑induced apoptosis was significantly reduced. Furthermore, the formation of autophagosomes, cytosolic microtubule‑associated protein 1A/1B‑light chain 3 ratio and beclin1 levels significantly increased following hypoxia/reoxygenation injury; however, all of these effects were ameliorated following pre‑ or post‑treatment with ALA. The results of the present study suggested that ALA may provide beneficial protection against hypoxia/reoxygenation‑induced injury via attenuation of apoptosis and autophagy in HUVECs.

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