Monocyte chemotactic protein-1 promotes the proliferation and invasion of osteosarcoma cells and upregulates the expression of AKT

Chen,Quanchi; Sun,Wei; Liao,Yuxin; Zeng,Hui; Shan,Liancheng; Yin,Fei; Wang,Zhuoying; Zhou,Zifei; Hua,Yingqi; Cai,Zhengdong

doi:10.3892/mmr.2015.3375

July-2015 Volume 12 Issue 1

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July-2015 Volume 12 Issue 1

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Monocyte chemotactic protein-1 promotes the proliferation and invasion of osteosarcoma cells and upregulates the expression of AKT

Authors:
- Quanchi Chen
- Wei Sun
- Yuxin Liao
- Hui Zeng
- Liancheng Shan
- Fei Yin
- Zhuoying Wang
- Zifei Zhou
- Yingqi Hua
- Zhengdong Cai
View Affiliations / Copyright

Affiliations: Department of Orthopedics, First People's Hospital, Shanghai Jiaotong University, Shanghai 200080, P.R. China, Department of Orthopedics, Tenth People's Hospital of Tongji University, Shanghai 200072, P.R. China

Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
Pages: 219-225
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Published online on: February 18, 2015

https://doi.org/10.3892/mmr.2015.3375
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Abstract

Monocyte chemotactic protein‑1 (MCP‑1/CCL2) is an important immune factor, which may be important in cancer progression by promoting proliferation, invasion, metastasis and the tumor microenvironment. Previous studies have demonstrated that CCL2 affects the proliferation of osteosarcoma cells via the RANKL signaling pathway. However, the underlying mechanisms remain to be elucidated. To investigate the role of CCL2 in osteosarcoma cells, MTT, spheroid forming, wound healing and transwell assays were performed to examine the proliferation and invasion abilities of the osteosarcoma cells. It was revealed that the high‑grade osteosarcoma cells exhibited increased expression levels of CCL2 compared with the low‑grade osteosarcoma cells (P<0.001). Furthermore, knockdown of CCL2 decreased the proliferation and invasion abilities of the osteosarcoma cells (P<0.01). These results suggested that the expression of CCL2 is high in high‑grade osteosarcoma cells and promotes the proliferation and invasion of osteosarcoma cells.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Monocyte chemotactic protein-1 promotes the proliferation and invasion of osteosarcoma cells and upregulates the expression of AKT

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