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Article

Optimized codon usage enhances the expression and immunogenicity of DNA vaccine encoding Taenia solium oncosphere TSOL18 gene

  • Authors:
    • Yuan‑Yuan Wang
    • Xue‑Lian Chang
    • Zhi‑Yong Tao
    • Xiao‑Li Wang
    • Yu‑Meng Jiao
    • Yong Chen
    • Wen‑Juan Qi
    • Hui Xia
    • Xiao‑Di Yang
    • Xin Sun
    • Ji‑Long Shen
    • Qiang Fang
  • View Affiliations / Copyright

    Affiliations: Department of Microbiology and Parasitology, Bengbu Medical College, Bengbu, Anhui 233030, P.R. China, Department of Pathobiology, Anhui Medical University, Hefei, Anhui 230032, P.R. China
  • Pages: 281-288
    |
    Published online on: February 24, 2015
       https://doi.org/10.3892/mmr.2015.3387
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Abstract

Cysticercosis due to larval cysts of Taenia solium, is a serious public health problem affecting humans in numerous regions worldwide. The oncospheral stage‑specific TSOL18 antigen is a promising candidate for an anti‑cysticercosis vaccine. It has been reported that the immunogenicity of the DNA vaccine may be enhanced through codon optimization of candidate genes. The aim of the present study was to further increase the efficacy of the cysticercosis DNA vaccine; therefore, a codon optimized recombinant expression plasmid pVAX1/TSOL18 was developed in order to enhance expression and immunogenicity of TSOL18. The gene encoding TSOL18 of Taenia solium was optimized, and the resulting opt‑TSOL18 gene was amplified and expressed. The results of the present study showed that the codon‑optimized TSOL18 gene was successfully expressed in CHO‑K1 cells, and immunized mice vaccinated with opt‑TSOL18 recombinant expression plasmids demonstrated opt‑TSOL18 expression in muscle fibers, as determined by immunohistochemistry. In addition, the codon‑optimized TSOL18 gene produced a significantly greater effect compared with that of TSOL18 and active spleen cells were markedly stimulated in vaccinated mice. 3H‑thymidine incorporation was significantly greater in the opt‑TSOL18 group compared with that of the TSOL18, pVAX and blank control groups (P<0.01). In conclusion, the eukaryotic expression vector containing the codon‑optimized TSOL18 gene was successfully constructed and was confirmed to be expressed in vivo and in vitro. The expression and immunogenicity of the codon‑optimized TSOL18 gene were markedly greater compared with that of the un‑optimized gene. Therefore, these results may provide the basis for an optimized TSOL18 gene vaccine against cysticercosis.
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1 

Shoji H, Hirai T, Shirakura T, et al: A case of cysticercosis with multiple lesions in the brain and femoral muscles. Kansenshogaku Zasshi. 87:608–612. 2013. View Article : Google Scholar : PubMed/NCBI

2 

Rasamoelina-Andriamanivo H, Porphyre V and Jambou R: Control of cysticercosis in Madagascar: beware of the pitfalls. Trends Parasitol. 29:538–547. 2013. View Article : Google Scholar : PubMed/NCBI

3 

Iacoangeli M, Moriconi E, Gladi M and Scerrati M: Isolated cysticercosis of the cauda equina. J Neurosci Rural Pract. 4(Suppl 1): S117–S119. 2013. View Article : Google Scholar : PubMed/NCBI

4 

Sciutto E, Fragoso G, Fleury A, et al: Taenia solium disease in humans and pigs: an ancient parasitosis disease rooted in developing countries and emerging as a major health problem of global dimensions. Microbes Infect. 2:1875–1890. 2000. View Article : Google Scholar

5 

Schantz PM, Cruz M, Sarti E and Pawlowski Z: Potential eradicability of taeniasis and cysticercosis. Bull Pan Am Health Organ. 27:397–403. 1993.PubMed/NCBI

6 

García HH, Gonzalez AE, Evans CA and Gilman RH; Cysticercosis Working Group in Peru: Taenia solium cysticercosis. Lancet. 362:547–556. 2003. View Article : Google Scholar : PubMed/NCBI

7 

Drew DR, Lightowlers M and Strugnell RA: Vaccination with plasmid DNA expressing antigen from genomic or cDNA gene forms induces equivalent humoral immune responses. Vaccine. 18:692–702. 1999. View Article : Google Scholar : PubMed/NCBI

8 

Solís CF, Ostoa-Saloma P, Lugo-Martínez VH, Johnston SA and Laclette JP: vaccination against murine cysticercosis by using a plasmid vector carrying Taenia solium paramyosin. Infect Immun. 73:1895–1897. 2005. View Article : Google Scholar

9 

Harrison GB, Heath DD, Dempster RP, et al: Identification and cDNA cloning of two novel low molecular weight host-protective antigens from Taenia ovis oncospheres. Int J Parasitol. 26:195–204. 1996. View Article : Google Scholar : PubMed/NCBI

10 

Lightowlers MW, Rolfe R and Gauci CG: Taenia saginata: vaccination against cysticercosis in cattle with recombinant oncosphere antigens. Exp Parasitol. 84:330–338. 1996. View Article : Google Scholar : PubMed/NCBI

11 

Lightowlers MW, Flisser A, Gauci CG, Heath DD, Jensen O and Rolfe R: Vaccination against cysticercosis and hydatid disease. Parasitol Today. 16:191–196. 2000. View Article : Google Scholar : PubMed/NCBI

12 

Rosas G, Fragoso G, Garate T, et al: Protective immunity against Taenia crassiceps murine cysticercosis induced by DNA vaccination with a Taenia saginata tegument antigen. Microbes Infect. 4:1417–1426. 2002. View Article : Google Scholar : PubMed/NCBI

13 

Martinez-Ocaña J, Romero-Valdovinos M, de Kaminsky RG, Maravilla P and Flisser A: Immunolocalization of TSOL 18 and TSOL 45-1A, the successful protective peptides against porcine cysticercosis, in Taenia solium oncospheres. Parasit Vectors. 4:32011. View Article : Google Scholar

14 

Flisser A, Gauci CG, Zoli A, et al: Induction of protection against porcine cysticercosis by vaccination with recombinant oncosphere antigens. Infect Immun. 72:5292–5297. 2004. View Article : Google Scholar : PubMed/NCBI

15 

Cai X, Yuan G, Zheng Y, et al: Effective production and purification of the glycosylated TSOL 18 antigen, which is protective against pig cysticercosis. Infect Immun. 76:767–770. 2008. View Article : Google Scholar :

16 

Zhou Z, Schnake P, Xiao L and Lal AA: Enhanced expression of a recombinant malaria candidate vaccine in Escherichia coli by codon optimization. Protein Expr Purif. 34:87–94. 2004. View Article : Google Scholar : PubMed/NCBI

17 

Villalobos A, Ness JE, Gustafsson C, et al: Gene Designer: a synthetic biology tool for constructing artificial DNA segments. BMC Bioinformatics. 7:2852006. View Article : Google Scholar : PubMed/NCBI

18 

Sharp PM and Devine KM: Codon usage and gene expression level in Dictyostelium discoideum: highly expressed genes do ‘prefer’ optimal codons. Nucleic Acids Res. 17:5029–5039. 1989. View Article : Google Scholar : PubMed/NCBI

19 

Gao Z, Li Z, Zhang Y, et al: High-level expression of the Penicillium notatum glucose oxidase gene in Pichia pastoris using codon optimization. Biotechnol Lett. 34:507–514. 2012. View Article : Google Scholar

20 

Lee MS, Hseu YC, Lai GH, et al: High yield expression in a recombinant E. coli of a codon optimized chicken anemia virus capsid protein VP1 useful for vaccine development. Microb Cell Fact. 10:562011. View Article : Google Scholar : PubMed/NCBI

21 

Gustafsson C, Govindarajan S and Minshull J: Codon bias and heterologous protein expression. Trends Biotechnol. 22:346–353. 2004. View Article : Google Scholar : PubMed/NCBI

22 

Bulmer M: The selection-mutation-drift theory of synonymous codon usage. Genetics. 129:897–907. 1991.PubMed/NCBI

23 

Zheng JL, Jian GB and Guo XF: Codon modification for improvement in prokaryotic expression of glycoprotein gene in rabies virus. Chin J Zoonoses. 26:403–407. 2010.

24 

Mani I, Singh V, Chaudhary DK, Somvanshi P and Negi MP: Codon optimization of the major antigen encoding genes of diverse strains of influenza a virus. Interdiscip Sci. 3:36–42. 2011. View Article : Google Scholar : PubMed/NCBI

25 

Raab D, Graf M, Notka F, Schödl T and Wagner R: The GeneOptimizer Algorithm: using a sliding window approach to cope with the vast sequence space in multiparameter DNA sequence optimization. Syst Synth Biol. 4:215–225. 2010. View Article : Google Scholar : PubMed/NCBI

26 

Wang JX, Peng YC and Yao YX: Stage-specific expression of antigen encoded cDNA of cysticercus cellulosae. Chin J Parasitol Parasit Dis. 2:89–93. 1996.

27 

Greene RM, Hancock K, Wilkins PP and Tsang VC: Taenia solium: molecular cloning and serologic evaluation of 14- and 18-kDa related, diagnostic antigens. J Parasitol. 86:1001–1007. 2000. View Article : Google Scholar : PubMed/NCBI

28 

Hiszczyńska-Sawicka E, Li H, Xu JB, et al: Comparison of immune response in sheep immunized with DNA vaccine encoding Toxoplasma gondii GRA7 antigen in different adjuvant formulations. Exp Parasitol. 124:365–372. 2010. View Article : Google Scholar

29 

Yang TC: Study on the transfection efficiency of the new cationic polymer transfection reagent: Sofast. Xia men da xue xue bao. 43:572–577. 2004.

30 

Khan KH: DNA vaccines: roles against diseases. Germs. 3:26–35. 2013. View Article : Google Scholar

31 

Vibanco-perez N, Jimenez L, Mendoza-Hernandez G and Landa A: Characterization of a recombinant mu-class glutathione stransferase from Taenia solium. Parasitol Res. 88:394–404. 2002.

32 

Rothel JS, Boyle DB, Both GW, et al: Sequential nucleic acid and recombinant adenovirus vaccination induces host-protective immune responses against Taenia ovis infection in sheep. Parasite Immunol. 19:221–227. 1997. View Article : Google Scholar : PubMed/NCBI

33 

Drew DR, Lightowlers M and Strugnell RA: Humoral immune responses to DNA vaccines expressing secreted, membrane bound and non-secreted forms of the Tania ovis 45W antigen. Vaccine. 18:2522–2532. 2000. View Article : Google Scholar : PubMed/NCBI

34 

Dong XX, Zhang C, Yang XW, et al: Activities of treg cells stimulated by soluble adult worm antigen and egg antigen of Schistosoma japonicum. Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi. 25:146–150. 2013.PubMed/NCBI

35 

Hwang J, Yi M, Zhang X, Xu Y, Jung JH and Kim DK: Cytochalasin B induces apoptosis through the mitochondrial apoptotic pathway in HeLa human cervical carcinoma cells. Oncol Rep. 30:1929–1935. 2013.PubMed/NCBI

36 

Weng SX, Sui MH, Chen S, et al: Parthenolide inhibits proliferation of vascular smooth muscle cells through induction of G0/G1 phase cell cycle arrest. Zhejiang Univ Sci B. 10:528–535. 2009. View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Wang YY, Chang XL, Tao ZY, Wang XL, Jiao YM, Chen Y, Qi WJ, Xia H, Yang XD, Sun X, Sun X, et al: Optimized codon usage enhances the expression and immunogenicity of DNA vaccine encoding Taenia solium oncosphere TSOL18 gene. Mol Med Rep 12: 281-288, 2015.
APA
Wang, Y., Chang, X., Tao, Z., Wang, X., Jiao, Y., Chen, Y. ... Fang, Q. (2015). Optimized codon usage enhances the expression and immunogenicity of DNA vaccine encoding Taenia solium oncosphere TSOL18 gene. Molecular Medicine Reports, 12, 281-288. https://doi.org/10.3892/mmr.2015.3387
MLA
Wang, Y., Chang, X., Tao, Z., Wang, X., Jiao, Y., Chen, Y., Qi, W., Xia, H., Yang, X., Sun, X., Shen, J., Fang, Q."Optimized codon usage enhances the expression and immunogenicity of DNA vaccine encoding Taenia solium oncosphere TSOL18 gene". Molecular Medicine Reports 12.1 (2015): 281-288.
Chicago
Wang, Y., Chang, X., Tao, Z., Wang, X., Jiao, Y., Chen, Y., Qi, W., Xia, H., Yang, X., Sun, X., Shen, J., Fang, Q."Optimized codon usage enhances the expression and immunogenicity of DNA vaccine encoding Taenia solium oncosphere TSOL18 gene". Molecular Medicine Reports 12, no. 1 (2015): 281-288. https://doi.org/10.3892/mmr.2015.3387
Copy and paste a formatted citation
x
Spandidos Publications style
Wang YY, Chang XL, Tao ZY, Wang XL, Jiao YM, Chen Y, Qi WJ, Xia H, Yang XD, Sun X, Sun X, et al: Optimized codon usage enhances the expression and immunogenicity of DNA vaccine encoding Taenia solium oncosphere TSOL18 gene. Mol Med Rep 12: 281-288, 2015.
APA
Wang, Y., Chang, X., Tao, Z., Wang, X., Jiao, Y., Chen, Y. ... Fang, Q. (2015). Optimized codon usage enhances the expression and immunogenicity of DNA vaccine encoding Taenia solium oncosphere TSOL18 gene. Molecular Medicine Reports, 12, 281-288. https://doi.org/10.3892/mmr.2015.3387
MLA
Wang, Y., Chang, X., Tao, Z., Wang, X., Jiao, Y., Chen, Y., Qi, W., Xia, H., Yang, X., Sun, X., Shen, J., Fang, Q."Optimized codon usage enhances the expression and immunogenicity of DNA vaccine encoding Taenia solium oncosphere TSOL18 gene". Molecular Medicine Reports 12.1 (2015): 281-288.
Chicago
Wang, Y., Chang, X., Tao, Z., Wang, X., Jiao, Y., Chen, Y., Qi, W., Xia, H., Yang, X., Sun, X., Shen, J., Fang, Q."Optimized codon usage enhances the expression and immunogenicity of DNA vaccine encoding Taenia solium oncosphere TSOL18 gene". Molecular Medicine Reports 12, no. 1 (2015): 281-288. https://doi.org/10.3892/mmr.2015.3387
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