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Article

Pirfenidone inhibits proliferation, arrests the cell cycle, and downregulates heat shock protein‑47 and collagen type I in rat hepatic stellate cells in vitro

  • Authors:
    • Xian‑Hong Xiang
    • Tian‑Peng Jiang
    • Shuai Zhang
    • Jie Song
    • Xing Li
    • Jian‑Yong Yang
    • Shi Zhou
  • View Affiliations / Copyright

    Affiliations: Department of Interventional Radiology, The First Affiliated Hospital of Sun Yat‑Sen University, Guangzhou, Guangdong 510080, P.R. China, Department of Interventional Radiology, Affiliated Hospital of Guiyang Medical College, Guiyang, Guizhou 550000, P.R. China, Department of Interventional Radiology, Affiliated Cancer Hospital of Guiyang Medical College, Guiyang, Guizhou 550000, P.R. China
  • Pages: 309-314
    |
    Published online on: March 3, 2015
       https://doi.org/10.3892/mmr.2015.3403
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Abstract

Pirfenidone (esbiret) is an established anti‑fibrotic and anti‑inflammatory drug used to treat idiopathic pulmonary fibrosis. In the present study, the dose‑dependent effects of pirfenidone on the cell cycle, proliferation and expression of heat shock protein (HSP)‑47 and collagen type I in a cultured rat hepatic stellate cell line (HSC‑T6) were investigated. Following pirfenidone treatment, cell proliferation was determined using the cell counting kit‑8 assay and the cell cycle was measured using flow cytometry. HSP‑47 expression was estimated using western blot analysis and collagen type I mRNA was assessed using reverse transcription quantitative polymerase chain reaction. Pirfenidone induced significant dose‑dependent inhibition of proliferation in HSC‑T6 cells. Cell viability was unaffected by treatment with pirfenidone (0, 10 or 100 µM) for 24 and 72 h. However, after 24 h, HSC‑T6 cells exhibited dose‑dependent decreases in HSP‑47 protein and collagen I mRNA levels. In conclusion, pirfenidone inhibited HSC‑T6 cell proliferation, arrested the cell cycle and reduced the expression of HSP‑47 and collagen type I, indicating that pirfenidone may be a promising drug in the treatment of liver fibrosis.
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Copy and paste a formatted citation
Spandidos Publications style
Xiang XH, Jiang TP, Zhang S, Song J, Li X, Yang JY and Zhou S: Pirfenidone inhibits proliferation, arrests the cell cycle, and downregulates heat shock protein‑47 and collagen type I in rat hepatic stellate cells in vitro. Mol Med Rep 12: 309-314, 2015.
APA
Xiang, X., Jiang, T., Zhang, S., Song, J., Li, X., Yang, J., & Zhou, S. (2015). Pirfenidone inhibits proliferation, arrests the cell cycle, and downregulates heat shock protein‑47 and collagen type I in rat hepatic stellate cells in vitro. Molecular Medicine Reports, 12, 309-314. https://doi.org/10.3892/mmr.2015.3403
MLA
Xiang, X., Jiang, T., Zhang, S., Song, J., Li, X., Yang, J., Zhou, S."Pirfenidone inhibits proliferation, arrests the cell cycle, and downregulates heat shock protein‑47 and collagen type I in rat hepatic stellate cells in vitro". Molecular Medicine Reports 12.1 (2015): 309-314.
Chicago
Xiang, X., Jiang, T., Zhang, S., Song, J., Li, X., Yang, J., Zhou, S."Pirfenidone inhibits proliferation, arrests the cell cycle, and downregulates heat shock protein‑47 and collagen type I in rat hepatic stellate cells in vitro". Molecular Medicine Reports 12, no. 1 (2015): 309-314. https://doi.org/10.3892/mmr.2015.3403
Copy and paste a formatted citation
x
Spandidos Publications style
Xiang XH, Jiang TP, Zhang S, Song J, Li X, Yang JY and Zhou S: Pirfenidone inhibits proliferation, arrests the cell cycle, and downregulates heat shock protein‑47 and collagen type I in rat hepatic stellate cells in vitro. Mol Med Rep 12: 309-314, 2015.
APA
Xiang, X., Jiang, T., Zhang, S., Song, J., Li, X., Yang, J., & Zhou, S. (2015). Pirfenidone inhibits proliferation, arrests the cell cycle, and downregulates heat shock protein‑47 and collagen type I in rat hepatic stellate cells in vitro. Molecular Medicine Reports, 12, 309-314. https://doi.org/10.3892/mmr.2015.3403
MLA
Xiang, X., Jiang, T., Zhang, S., Song, J., Li, X., Yang, J., Zhou, S."Pirfenidone inhibits proliferation, arrests the cell cycle, and downregulates heat shock protein‑47 and collagen type I in rat hepatic stellate cells in vitro". Molecular Medicine Reports 12.1 (2015): 309-314.
Chicago
Xiang, X., Jiang, T., Zhang, S., Song, J., Li, X., Yang, J., Zhou, S."Pirfenidone inhibits proliferation, arrests the cell cycle, and downregulates heat shock protein‑47 and collagen type I in rat hepatic stellate cells in vitro". Molecular Medicine Reports 12, no. 1 (2015): 309-314. https://doi.org/10.3892/mmr.2015.3403
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