Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
July-2015 Volume 12 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
July-2015 Volume 12 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Attenuation of enoyl coenzyme A hydratase 1 expression in colorectal cancer cells using small interfering RNA inhibits cell proliferation and migration

  • Authors:
    • Qing‑Mei Zhao
    • Fei Kuang
    • Han Wu
    • Yu-Hao Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Oncology, Sichuan Mianyang 404 Hospital, Mianyang, Sichuan 621000, P.R. China, Department of General Surgery, Changhai Hospital of The Second Military Medical University, Shanghai 200433, P.R. China, Department of General Surgery, The 1745th Hospital of the PLA, Zhangzhou, Fujian 562001, P.R. China
  • Pages: 470-474
    |
    Published online on: March 4, 2015
       https://doi.org/10.3892/mmr.2015.3418
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Colorectal cancer is one of the most commonly diagnosed types of cancer and is a leading cause of cancer‑associated mortality worldwide. Short chain enoyl coenzyme A hydratase 1 (ECHS1) is an important gene involved in the mitochondrial fatty acid β‑oxidation pathway. In addition, ECHS1 has been implicated in a variety of cancers, including breast, prostate, colon and liver cancer. The aim of the present study was to examine the expression of ECHS1 in the human HCT‑8 colorectal cancer cell line. The results showed that ECHS1 expression was significantly increased in poorly‑differentiated cells compared with that in well‑differentiated cells. In order to further investigate the functions of ECHS1 in colorectal cancer cells, a stably transfected HCT‑8 cell line expressing small interfering (si)RNA targeting the ECHS1 gene was established. The expression of the ECHS1 siRNA was found to reduce ECHS1 protein levels in ECHS1‑silenced cells by >40%. Cell proliferation and cell migration of the siECHS1 cells were characterized using Cell Counting Kit‑8 and Transwell assays, respectively, the results of which showed that the constitutive knockdown of the ECSH1 gene in HCT‑8 cells significantly inhibited cell proliferation and migration. Furthermore, decreased levels of Akt and glycogen synthase kinase (GSK)3β phosphorylation were observed in ECHS1‑silenced HCT‑8 cells compared with that of parental or pU6 empty vector‑transfected cells. In conclusion, the results of the present study suggested that ECHS1 may have an important role in colorectal cancer cell proliferation and migration via activation of Akt‑ and GSK3β‑associated signaling pathways.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

Orannapalai N, Attawettayanon W, Kanngern S, Boonpipattanapong T and Sangkhathat S: Predicting the occurrence of cancer-associated colorectal polyp using a metabolic risk score. Mol Clin Oncol. 2:124–128. 2014.PubMed/NCBI

2 

Zhang GJ, Zhou T, Liu ZL, Tian HP and Xia SS: Plasma miR-200c and miR-18a as potential biomarkers for the detection of colorectal carcinoma. Mol Clin Oncol. 1:379–384. 2013.

3 

Chen G, Mao B, Pan Q, Liu Q, Xu X and Ning Y: Prediction rule for estimating advanced colorectal neoplasm risk in average-risk populations in southern Jiangsu Province. Chin J Cancer Res. 26:4–11. 2014.PubMed/NCBI

4 

Crncec I, Pathria P, Svinka J and Eferl R: Induction of colorectal cancer in mice and hitomorphometric evaluation of tumors. Methods Mol Biol. 1267:145–164. 2015.

5 

Zhang J, Sun M, Li R, Liu S, Mao J, Huang Y, Wang B, Hou L, Ibrahim MM and Tang J: Ech1 is a potent suppressor of lymphatic metastasis in hepatocarcinoma. Biomed Pharmacother. 67:557–560. 2013. View Article : Google Scholar : PubMed/NCBI

6 

Janssen U, Davis EM, Le Beau MM and Stoffel W: Human mitochondrial enoyl-CoA hydratase gene (ECHS1): structural organization and assignment to chromosome 10q26.2–q26.3. Genomics. 40:470–475. 1997. View Article : Google Scholar : PubMed/NCBI

7 

Sakai C, Yamaguchi S, Sasaki M, Miyamoto Y, Matsushima Y and Goto Y: ECHS1 mutations cause combined respiratory chain deficiency resulting in Leigh syndrome. Hum Mutat. 36:232–239. 2015. View Article : Google Scholar

8 

Zhang X, Yang J, Guo Y, Ye H, Yu C, Xu C, Xu L, Wu S, Sun W, Wei H, Gao X, Zhu Y, Qian X, Jiang Y, Li Y and He F: Functional proteomic analysis of nonalcoholic fatty liver disease in rat models: enoylcoenzyme a hydratase down-regulation exacerbates hepatic steatosis. Hepatology. 51:1190–1199. 2010. View Article : Google Scholar : PubMed/NCBI

9 

Liu X, Feng R and Du L: The role of enoyl-CoA hydratase short chain 1 and peroxiredoxin 3 in PP2-induced apoptosis in human breast cancer MCF-7 cells. FEBS Lett. 584:3185–3192. 2010. View Article : Google Scholar : PubMed/NCBI

10 

Gong X, Zhu Y, Dong J, Chen J, You J, Zheng Q, Rao Z, Mao Q and Jiang J: Small hepatitis B surface antigen interacts with and modulates enoyl-coenzyme A hydratase expression in hepatoma cells. Arch Virol. 158:1065–1070. 2013. View Article : Google Scholar : PubMed/NCBI

11 

Chang Y, Wang SX, Wang YB, Zhou J, Li WH, Wang N, Fang DF, Li HY, Li AL, Zhang XM and Zhang WN: ECHS1 interacts with STAT3 and negatively regulates STAT3 signaling. FEBS Lett. 587:607–613. 2013. View Article : Google Scholar : PubMed/NCBI

12 

Yang G, Ma F, Zhong M, Fang L, Peng Y, Xin X, Zhong J, Zhu W and Zhang Y: Interleukin-11 induces the expression of matrix metalloproteinase 13 in gastric cancer SCH cells partly via the PI3K-AKT and JAK-STAT3 pathways. Mol Med Rep. 9:1371–1375. 2014.PubMed/NCBI

13 

Von Ohlen T, Luce-Fedrow A, Ortega MT, Ganta RR and Chapes SK: Identification of critical host mitochondrion-associated genes during Ehrlichia chaffeensis infections. Infect Immun. 80:3576–3586. 2012. View Article : Google Scholar : PubMed/NCBI

14 

Zhu XS, Gao P, Dai YC, Xie JP, Zeng W and Lian QN: Attenuation of enoyl coenzyme A hydratase short chain 1 expression in gastric cancer cells inhibits cell proliferation and migration in vitro. Cell Mol Biol Lett. 19:576–589. 2014. View Article : Google Scholar : PubMed/NCBI

15 

Zhu XS, Dai YC, Chen ZX, Xie JP, Zeng W, Lin YY and Tan QH: Knockdown of ECHS1 protein expression inhibits hepatocellular carcinoma cell proliferation via suppression of Akt activity. Crit Rev Eukaryot Gene Expr. 23:275–282. 2013. View Article : Google Scholar : PubMed/NCBI

16 

Xie JP, Zhu XS, Dai YC, Yu C, Xie T and Chen ZX: Expression of enoyl coenzyme A hydratase, short chain, 1, in colorectal cancer and its association with clinicopathological characteristics. Mol Clin Oncol. 2:1081–1084. 2014.PubMed/NCBI

17 

Zhang L, Zhang J, Dong Y, Swain CA, Zhang Y and Xie Z: The potential dual effects of sevoflurane on AKT/GSK3beta signaling pathway. Med Gas Res. 4:52014. View Article : Google Scholar

18 

Chen L, Wei X, Hou Y, Liu X, Li S, Sun B, Liu X and Liu H: Tetramethylpyrazine analogue CXC195 protects against cerebral ischemia/reperfusion-induced apoptosis through PI3 K/Akt/GSK3β pathway in rats. Neurochem Int. 66:27–32. 2014. View Article : Google Scholar : PubMed/NCBI

19 

Zhou RJ, Wang F, Zhang XH, Zhang JJ, Xu J, Dong W and Zou ZQ: A-eleostearic acid inhibits growth and induces apoptosis in breast cancer cells via HER2/HER3 signaling. Mol Med Rep. 9:993–998. 2014.

20 

Wang R, Li Z, Guo H, Shi W, Xin Y, Chang W and Huang T: Caveolin 1 knockdown inhibits the proliferation, migration and invasion of human breast cancer BT474 cells. Mol Med Rep. 9:1723–1728. 2014.PubMed/NCBI

21 

Yunqiao L, Vanke H, Jun X and Tangmeng G: MicroRNA-206, down-regulated in hepatocellular carcinoma, suppresses cell proliferation and promotes apoptosis. Hepatogastroenterology. 61:1302–1307. 2014.PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Zhao QM, Kuang F, Wu H and Zhang Y: Attenuation of enoyl coenzyme A hydratase 1 expression in colorectal cancer cells using small interfering RNA inhibits cell proliferation and migration. Mol Med Rep 12: 470-474, 2015.
APA
Zhao, Q., Kuang, F., Wu, H., & Zhang, Y. (2015). Attenuation of enoyl coenzyme A hydratase 1 expression in colorectal cancer cells using small interfering RNA inhibits cell proliferation and migration. Molecular Medicine Reports, 12, 470-474. https://doi.org/10.3892/mmr.2015.3418
MLA
Zhao, Q., Kuang, F., Wu, H., Zhang, Y."Attenuation of enoyl coenzyme A hydratase 1 expression in colorectal cancer cells using small interfering RNA inhibits cell proliferation and migration". Molecular Medicine Reports 12.1 (2015): 470-474.
Chicago
Zhao, Q., Kuang, F., Wu, H., Zhang, Y."Attenuation of enoyl coenzyme A hydratase 1 expression in colorectal cancer cells using small interfering RNA inhibits cell proliferation and migration". Molecular Medicine Reports 12, no. 1 (2015): 470-474. https://doi.org/10.3892/mmr.2015.3418
Copy and paste a formatted citation
x
Spandidos Publications style
Zhao QM, Kuang F, Wu H and Zhang Y: Attenuation of enoyl coenzyme A hydratase 1 expression in colorectal cancer cells using small interfering RNA inhibits cell proliferation and migration. Mol Med Rep 12: 470-474, 2015.
APA
Zhao, Q., Kuang, F., Wu, H., & Zhang, Y. (2015). Attenuation of enoyl coenzyme A hydratase 1 expression in colorectal cancer cells using small interfering RNA inhibits cell proliferation and migration. Molecular Medicine Reports, 12, 470-474. https://doi.org/10.3892/mmr.2015.3418
MLA
Zhao, Q., Kuang, F., Wu, H., Zhang, Y."Attenuation of enoyl coenzyme A hydratase 1 expression in colorectal cancer cells using small interfering RNA inhibits cell proliferation and migration". Molecular Medicine Reports 12.1 (2015): 470-474.
Chicago
Zhao, Q., Kuang, F., Wu, H., Zhang, Y."Attenuation of enoyl coenzyme A hydratase 1 expression in colorectal cancer cells using small interfering RNA inhibits cell proliferation and migration". Molecular Medicine Reports 12, no. 1 (2015): 470-474. https://doi.org/10.3892/mmr.2015.3418
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team