Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
July-2015 Volume 12 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
July-2015 Volume 12 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Cluster of differentiation 147 is a key molecule during hepatocellular carcinoma cell‑hepatic stellate cell cross‑talk in the rat liver

  • Authors:
    • Tianyou Ma
    • Zhilun Wang
    • Zhantian Yang
    • Jinghong Chen
  • View Affiliations / Copyright

    Affiliations: Institute of Endemic Diseases, Environment Related Gene Key Laboratory of Ministry of Education, Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China
    Copyright: © Ma et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 111-118
    |
    Published online on: March 4, 2015
       https://doi.org/10.3892/mmr.2015.3429
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The cross‑talk between hepatocellular carcinoma (HCC) cells and activated hepatic stellate cells (HSCs) is considered to be important for modulating the biological behavior of tumor cells. However, the molecular links between inflammation and cancer in the activation of HSCs remain to be elucidated. The present study demonstrated that cluster of differentiation (CD)147 is a key molecule involved in the interaction between HCC cells and HSCs. The effects of conditioned medium from human HCC cells on the activation of the human HSC line, LX‑2, were assessed using a 3‑(4,5‑dimethylthiazol‑2‑yl)‑2,5‑diphenyltetrazolium bromide assay, western blotting and reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR). Western blotting, RT‑qPCR and gelatin zymography were also used to investigate the effects of CD147 on the activation of LX‑2. The expression levels of α‑smooth muscle actin (α‑SMA) and CD147 were assessed in a co‑culture system of LX‑2 and FHCC‑98 cells by immunofluorescence staining and immunoblotting. In hepatic tissues from a rat model of fibrosis, immunohistochemistry and immunoblotting were performed to detect the expression levels of α‑SMA and CD147. Tumor‑conditioned medium and CD147 promoted cell proliferation, activated LX‑2 cells, increased the expression levels of α‑SMA, collagen I and tissue inhibitor of metalloproteinase‑1 (TIMP‑1), and increased the secretion of matrix metalloproteinase (MMP)‑2. The HSCs, which were induced in the co‑culture system of HCC cells and HSCs exhibited marked expression levels of CD147. In the hepatic tissue of rat models of fibrosis induced by CCl4, marked expression levels of CD147 were observed in the activated HSCs. Therefore, CD147 promoted the activation of HSCs and was a key molecule during HCC cell‑HSC cross‑talk in the rat liver.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

Mikula M, Proell V, Fischer AM and Mikulits W: Activated hepatic stellate cells induce tumor progression of neoplastic hepatocytes in a TGF-beta dependent fashion. J Cell Physiol. 209:560–567. 2006. View Article : Google Scholar : PubMed/NCBI

2 

Bataller R and Brenner DA: Liver fibrosis. J Clin Invest. 115:209–218. 2005. View Article : Google Scholar : PubMed/NCBI

3 

Friedman SL: Mechanisms of hepatic fibrogenesis. Gastroenterology. 134:1655–1669. 2008. View Article : Google Scholar : PubMed/NCBI

4 

Guyot C, Lepreux S, Combe C, et al: Hepatic fibrosis and cirrhosis: The (myo)fibroblastic cell subpopulations involved. Int J Biochem Cell Biol. 38:135–151. 2006.

5 

Friedman SL: Hepatic stellate cells: Protean, multifunctional, and enigmatic cells of the liver. Physiol Rev. 88:125–172. 2008. View Article : Google Scholar : PubMed/NCBI

6 

Novo E, di Bonzo LV, Cannito S, et al: Hepatic myofibroblasts: a heterogeneous population of multifunctional cells in liver fibrogenesis. Int J Biochem Cell Biol. 41:2089–2093. 2009. View Article : Google Scholar : PubMed/NCBI

7 

Coulouarn C, Corlu A, Glaise D, et al: Hepatocyte-stellate cell cross-talk in the liver engenders a permissive inflammatory microenvironment that drives progression in hepatocellular carcinoma. Cancer Res. 72:2533–2542. 2012. View Article : Google Scholar : PubMed/NCBI

8 

Wang BB, Cheng JY, Gao HH, et al: Hepatic stellate cells in inflammation-fibrosis-carcinoma axis. Anat Rec (Hoboken). 293:1492–1496. 2010. View Article : Google Scholar

9 

Zhao W, Zhang L, Yin Z, et al: Activated hepatic stellate cells promote hepatocellular carcinoma development in immunocompetent mice. Int J Cancer. 129:2651–2661. 2011. View Article : Google Scholar : PubMed/NCBI

10 

Amann T, Bataille F, Spruss T, et al: Activated hepatic stellate cells promote tumorigenicity of hepatocellular carcinoma. Cancer Sci. 100:646–653. 2009. View Article : Google Scholar : PubMed/NCBI

11 

Jung JO, Gwak GY, Lim YS, et al: Role of hepatic stellate cells in the angiogenesis of hepatoma. Korean J Gastroenterol. 42:142–148. 2003.In Korean. PubMed/NCBI

12 

Xia Y, Chen R, Song Z, et al: Gene expression profiles during activation of cultured rat hepatic stellate cells by tumoral hepatocytes and fetal bovine serum. J Cancer Res Clin Oncol. 136:309–321. 2010. View Article : Google Scholar

13 

Sancho-Bru P, Juez E, Moreno M, et al: Hepatocarcinoma cells stimulate the growth, migration and expression of pro-angiogenic genes in human hepatic stellate cells. Liver Int. 30:31–41. 2010. View Article : Google Scholar

14 

Xia YH, Song ZJ, Chen RX, et al: Analysis of differential gene expression in rat hepatic stellate cells activated by culture or hepatocellular carcinoma cell induction. Zhonghua Zhong Liu Za Zhi. 31:164–169. 2009.PubMed/NCBI

15 

Nagoshi S: Liver diseases. Nihon Rinsho. 72:726–729. 2014.In Japanese. PubMed/NCBI

16 

Tahashi Y, Matsuzaki K, Date M, et al: Differential regulation of TGF-beta signal in hepatic stellate cells between acute and chronic liver injury. Hepatology. 35:49–61. 2002. View Article : Google Scholar : PubMed/NCBI

17 

Wang J, Zohar R and McCulloch CA: Multiple roles of alpha-smooth muscle actin in mechanotransduction. Exp Cell Res. 312:205–214. 2006. View Article : Google Scholar

18 

Ooi LL, Bay BH, Ng RT, et al: An animal model for the study of hepatic stellate cell and hepatocellular carcinoma interaction. Ann Acad Med Singapore. 28:95–98. 1999.PubMed/NCBI

19 

Kurogi M, Nakashima O, Miyaaki H, et al: Clinicopathological study of scirrhous hepatocellular carcinoma. J Gastroenterol Hepatol. 21:1470–1477. 2006.PubMed/NCBI

20 

Zhao W, Zhang L, Xu Y, et al: Hepatic stellate cells promote tumor progression by enhancement of immunosuppressive cells in an orthotopic liver tumor mouse model. Lab Invest. 94:182–191. 2014. View Article : Google Scholar

21 

Han S, Han L, Yao Y, et al: Activated hepatic stellate cells promote hepatocellular carcinoma cell migration and invasion via the activation of FAK-MMP9 signaling. Oncol Rep. 31:641–648. 2014.

22 

Nevzorova YA, Hu W, Cubero FJ, et al: Overexpression of c-myc in hepatocytes promotes activation of hepatic stellate cells and facilitates the onset of liver fibrosis. Biochim Biophys Acta. 1832:1765–1775. 2013. View Article : Google Scholar : PubMed/NCBI

23 

Zhang DW, Zhao YX, Wei D, et al: H Ab18G/CD147 promotes activation of hepatic stellate cells and is a target for antibody therapy of liver fibrosis. J Hepatol. 57:1283–1291. 2012. View Article : Google Scholar : PubMed/NCBI

24 

Muramatsu T and Miyauchi T: Basigin (CD147): a multifunctional transmembrane protein involved in reproduction, neural function, inflammation and tumor invasion. Histol Histopathol. 18:981–987. 2003.PubMed/NCBI

25 

Weidle UH, Scheuer W, Eggle D, et al: Cancer-related issues of CD147. Cancer Genomics Proteomics. 7:157–169. 2010.PubMed/NCBI

26 

Hao JL, Cozzi PJ, Khatri A, et al: C D147/EMMPRIN and CD44 are potential therapeutic targets for metastatic prostate cancer. Curr Cancer Drug Targets. 10:287–306. 2010. View Article : Google Scholar : PubMed/NCBI

27 

Zhu S, Li Y, Zhang Y, et al: Expression and clinical implications of H Ab18G/CD147 in hepatocellular carcinoma. Hepatol Res. 45:97–106. 2015. View Article : Google Scholar

28 

Faouzi S, Lepreux S, Bedin C, et al: Activation of cultured rat hepatic stellate cells by tumoral hepatocytes. Lab Invest. 79:485–493. 1999.PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Ma T, Wang Z, Yang Z and Chen J: Cluster of differentiation 147 is a key molecule during hepatocellular carcinoma cell‑hepatic stellate cell cross‑talk in the rat liver. Mol Med Rep 12: 111-118, 2015.
APA
Ma, T., Wang, Z., Yang, Z., & Chen, J. (2015). Cluster of differentiation 147 is a key molecule during hepatocellular carcinoma cell‑hepatic stellate cell cross‑talk in the rat liver. Molecular Medicine Reports, 12, 111-118. https://doi.org/10.3892/mmr.2015.3429
MLA
Ma, T., Wang, Z., Yang, Z., Chen, J."Cluster of differentiation 147 is a key molecule during hepatocellular carcinoma cell‑hepatic stellate cell cross‑talk in the rat liver". Molecular Medicine Reports 12.1 (2015): 111-118.
Chicago
Ma, T., Wang, Z., Yang, Z., Chen, J."Cluster of differentiation 147 is a key molecule during hepatocellular carcinoma cell‑hepatic stellate cell cross‑talk in the rat liver". Molecular Medicine Reports 12, no. 1 (2015): 111-118. https://doi.org/10.3892/mmr.2015.3429
Copy and paste a formatted citation
x
Spandidos Publications style
Ma T, Wang Z, Yang Z and Chen J: Cluster of differentiation 147 is a key molecule during hepatocellular carcinoma cell‑hepatic stellate cell cross‑talk in the rat liver. Mol Med Rep 12: 111-118, 2015.
APA
Ma, T., Wang, Z., Yang, Z., & Chen, J. (2015). Cluster of differentiation 147 is a key molecule during hepatocellular carcinoma cell‑hepatic stellate cell cross‑talk in the rat liver. Molecular Medicine Reports, 12, 111-118. https://doi.org/10.3892/mmr.2015.3429
MLA
Ma, T., Wang, Z., Yang, Z., Chen, J."Cluster of differentiation 147 is a key molecule during hepatocellular carcinoma cell‑hepatic stellate cell cross‑talk in the rat liver". Molecular Medicine Reports 12.1 (2015): 111-118.
Chicago
Ma, T., Wang, Z., Yang, Z., Chen, J."Cluster of differentiation 147 is a key molecule during hepatocellular carcinoma cell‑hepatic stellate cell cross‑talk in the rat liver". Molecular Medicine Reports 12, no. 1 (2015): 111-118. https://doi.org/10.3892/mmr.2015.3429
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team