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Article Open Access

Fully human HER2/cluster of differentiation 3 bispecific antibody triggers potent and specific cytotoxicity of T lymphocytes against breast cancer

  • Authors:
    • Yan Zhou
    • Lan‑Tu Gou
    • Zhi‑Hui Guo
    • Hai‑Rong Liu
    • Jiang‑Man Wang
    • Shu‑Xian Zhou
    • Jin‑Liang Yang
    • Xiao‑An Li
  • View Affiliations / Copyright

    Affiliations: Gastroenterology Tumor and Microenvironment Laboratory, Department of Gastroenterology, The First Affiliated Hospital of Chengdu Medical College, Chengdu, Sichuan 610041, P.R. China, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China
    Copyright: © Zhou et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 147-154
    |
    Published online on: March 5, 2015
       https://doi.org/10.3892/mmr.2015.3441
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Abstract

The use of a bispecific antibody (BsAb) is a promising and highly specific approach to cancer therapy. In the present study, a fully human recombinant single chain variable fragment BsAb against human epidermal growth factor receptor (HER)2 and cluster of differentiation (CD)3 was constructed with the aim of developing an effective treatment for breast cancer. HER2/CD3 BsAb was expressed in Chinese hamster ovary cells and purified via nickel column chromatography. Flow cytometry revealed that the HER2/CD3 BsAb was able to specifically bind to HER2 and CD3‑positive cells. HER2/CD3 BsAb was able to stimulate T-cell activation and induce the lysis of cultured SKBR‑3 and BT474 cells in the presence of unstimulated T lymphocytes. HER2/CD3 BsAb efficiently inhibited the growth of breast cancer tissue by activating and inducing the proliferation of tumor tissue infiltrating lymphocytes. Therefore, HER2/CD3 BsAb is a potent tool which may be a suitable candidate for the treatment of breast cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Zhou Y, Gou LT, Guo ZH, Liu HR, Wang JM, Zhou SX, Yang JL and Li XA: Fully human HER2/cluster of differentiation 3 bispecific antibody triggers potent and specific cytotoxicity of T lymphocytes against breast cancer. Mol Med Rep 12: 147-154, 2015.
APA
Zhou, Y., Gou, L., Guo, Z., Liu, H., Wang, J., Zhou, S. ... Li, X. (2015). Fully human HER2/cluster of differentiation 3 bispecific antibody triggers potent and specific cytotoxicity of T lymphocytes against breast cancer. Molecular Medicine Reports, 12, 147-154. https://doi.org/10.3892/mmr.2015.3441
MLA
Zhou, Y., Gou, L., Guo, Z., Liu, H., Wang, J., Zhou, S., Yang, J., Li, X."Fully human HER2/cluster of differentiation 3 bispecific antibody triggers potent and specific cytotoxicity of T lymphocytes against breast cancer". Molecular Medicine Reports 12.1 (2015): 147-154.
Chicago
Zhou, Y., Gou, L., Guo, Z., Liu, H., Wang, J., Zhou, S., Yang, J., Li, X."Fully human HER2/cluster of differentiation 3 bispecific antibody triggers potent and specific cytotoxicity of T lymphocytes against breast cancer". Molecular Medicine Reports 12, no. 1 (2015): 147-154. https://doi.org/10.3892/mmr.2015.3441
Copy and paste a formatted citation
x
Spandidos Publications style
Zhou Y, Gou LT, Guo ZH, Liu HR, Wang JM, Zhou SX, Yang JL and Li XA: Fully human HER2/cluster of differentiation 3 bispecific antibody triggers potent and specific cytotoxicity of T lymphocytes against breast cancer. Mol Med Rep 12: 147-154, 2015.
APA
Zhou, Y., Gou, L., Guo, Z., Liu, H., Wang, J., Zhou, S. ... Li, X. (2015). Fully human HER2/cluster of differentiation 3 bispecific antibody triggers potent and specific cytotoxicity of T lymphocytes against breast cancer. Molecular Medicine Reports, 12, 147-154. https://doi.org/10.3892/mmr.2015.3441
MLA
Zhou, Y., Gou, L., Guo, Z., Liu, H., Wang, J., Zhou, S., Yang, J., Li, X."Fully human HER2/cluster of differentiation 3 bispecific antibody triggers potent and specific cytotoxicity of T lymphocytes against breast cancer". Molecular Medicine Reports 12.1 (2015): 147-154.
Chicago
Zhou, Y., Gou, L., Guo, Z., Liu, H., Wang, J., Zhou, S., Yang, J., Li, X."Fully human HER2/cluster of differentiation 3 bispecific antibody triggers potent and specific cytotoxicity of T lymphocytes against breast cancer". Molecular Medicine Reports 12, no. 1 (2015): 147-154. https://doi.org/10.3892/mmr.2015.3441
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