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Article

αB-crystallin reduces ristocetin‑induced soluble CD40 ligand release in human platelets: Suppression of thromboxane A2 generation

  • Authors:
    • Masanori Tsujimoto
    • Tomoaki Doi
    • Gen Kuroyanagi
    • Naohiro Yamamoto
    • Rie Matsushima‑Nishiwaki
    • Yuko Iida
    • Yukiko Enomoto
    • Hiroki Iida
    • Shinji Ogura
    • Takanobu Otsuka
    • Haruhiko Tokuda
    • Osamu Kozawa
    • Toru Iwama
  • View Affiliations / Copyright

    Affiliations: Department of Neurosugery, Gifu University Graduate School of Medicine, Gifu, Gifu 501‑1194, Japan, Department of Emergency and Disaster Medicine, Gifu University Graduate School of Medicine, Gifu, Gifu 501‑1194, Japan, Department of Pharmacology, Gifu University Graduate School of Medicine, Gifu, Gifu 501‑1194, Japan, Department of Anesthesiology and Pain Medicine, Gifu University Graduate School of Medicine, Gifu, Gifu 501‑1194, Japan, Department of Orthopedic Surgery, Nagoya University Graduate School of Medical Sciences, Nagoya, Aichi 467‑8603, Japan
  • Pages: 357-362
    |
    Published online on: March 6, 2015
       https://doi.org/10.3892/mmr.2015.3445
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Abstract

Our group has previously shown that αB‑crystallin (HSPB5), a small heat shock protein, inhibits human platelet aggregation by ristocetin, an activator of glycoprotein Ib/IX/V. In addition, it was demonstrated that glycoprotein Ib/IX/V activation induces soluble CD40 (sCD40) ligand release via thromboxane (TX) A2. In the present study, the effect of αB‑crystallin on the ristocetin‑induced sCD40 ligand release in human platelets was investigated. The ristocetin‑induced release of sCD40 ligand was suppressed by αB‑crystallin. In addition, αB‑crystallin reduced the ristocetin‑stimulated production of 11‑dehydro‑TX B2, a stable metabolite of TXA2. αB‑crystallin did not suppress the platelet aggregation induced by U46619, a TXA2 receptor agonist. αB‑crystallin had little effect on the U46619‑induced phosphorylation of p38 mitogen‑activated protein kinase or sCD40 ligand release. In addition, αB‑crystallin failed to reduce the binding of SZ2, a monoclonal antibody against the sulfated sequence in the α‑chain of glycoprotein Ib, to the ristocetin‑stimulated platelets. These results strongly suggest that αB‑crystallin extracellularly suppresses ristocetin‑stimulated release of sCD40 ligand by inhibiting the TXA2 production in human platelets.
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1 

Mymrikov EV, Seit-Nebi AS and Gusev NB: Large potentials of small heat shock proteins. Physiol Rev. 91:1123–1159. 2011. View Article : Google Scholar : PubMed/NCBI

2 

Taylor RP and Benjamin IJ: Small heat shock proteins: a new classification scheme in mammals. J Mol Cell Cardiol. 38:433–444. 2005. View Article : Google Scholar : PubMed/NCBI

3 

Kato K, Shinohara H, Goto S, Inaguma Y, Morishita R and Asano T: Copurification of small heat shock protein with αB crystallin from human skeletal muscle. J Biol Chem. 267:7718–7725. 1992.PubMed/NCBI

4 

Kato K, Hasegawa K, Goto S and Inaguma Y: Dissociation as a result of phosphorylation of an aggregated form of the small stress protein, hsp27. J Biol Chem. 269:11274–11278. 1994.PubMed/NCBI

5 

Kato K, Goto S, Inaguma Y, Hasegawa K, Morishita R and Asano T: Purification and characterization of a 20-kDa protein that is highly homologous to a B crystallin. J Biol Chem. 269:15302–15309. 1994.PubMed/NCBI

6 

Vos MJ, Hageman J, Carra S and Kampinga HH: Structural and functional diversities between members of the human HSPB, HSPH, HSPA and DNAJ chaperone families. Biochemistry. 47:7001–7011. 2008. View Article : Google Scholar : PubMed/NCBI

7 

Berndt MC, Shen Y, Dopheide SM, Gardiner EE and Andrews RK: The vascular biology of the glycoprotein Ib-IX-V complex. Thromb Haemost. 86:178–188. 2001.PubMed/NCBI

8 

Garcia A, Quinton TM, Dorsam RT and Kunapuli SP: Src family kinase-mediated and Erk-mediated thromboxane A2 generation are essential for vWF/GPIb-induced fibrinogen receptor activation in human platelets. Blood. 106:3410–3414. 2005. View Article : Google Scholar : PubMed/NCBI

9 

Dong JF, Berndt MC, Schade A, McIntire LV, Andrews RK and Lopez JA: Ristocetin-dependent, but not botrocetin-dependent, binding of von Willebrand factor to the platelet glycoprotein Ib-IX-V complex correlates with shear-dependent interactions. Blood. 97:162–168. 2001. View Article : Google Scholar : PubMed/NCBI

10 

Enomoto Y, Adachi S, Matsushima-Nishiwaki R, Doi T, Niwa M, Akamatsu S, et al: Thromboxane A2 promotes soluble CD40 ligand release from human platelets. Atherosclerosis. 209:415–421. 2010. View Article : Google Scholar

11 

Kozawa O, Matsuno H, Niwa M, Hatakeyama D, Kato K and Uematsu T: αB-crystallin, a low-molecular-weight heat shock protein, acts as a regulator of platelet function. Cell Stress Chaperones. 6:21–28. 2001. View Article : Google Scholar : PubMed/NCBI

12 

Matsuno HI, Ishisaki A, Nakajima K, Kato K and Kozawa O: A peptide isolated from αB-crystallin is a novel and potent inhibitor of platelet aggregation via dual prevention of PAR-1 and GPIb/V/IX. J Thromb Haemost. 12:2636–2642. 2003. View Article : Google Scholar

13 

Enomoto Y, Adachi S, Matsushima-Nishiwaki R, Niwa M, Tokuda H, Akamatsu S, et al: Alpha B-crystallin extracellularly suppresses ADP-induced granule secretion from human platelets. FEBS Lett. 583:2464–2468. 2009. View Article : Google Scholar : PubMed/NCBI

14 

Ward CM, Andrews RK, Smith AI and Berndt MC: Mocarhagin, a novel cobra venom metalloproteinase, cleaves the platelet von Willebrand factor receptor glycoprotein Iba. Identification of the sulfated tyrosine/anionic sequence Tyr-276-Glu-282 of glycoprotein Iba as a binding site for von Willebrand factor and a-thrombin. Biochemistry. 35:4929–4938. 1996. View Article : Google Scholar : PubMed/NCBI

15 

Fabre JE, Nguyen M, Latour A, Keifer JA, Audoly LP, Coffman TM and Koller BH: Decreased platelet aggregation, increased bleeding time and resistance to thromboembolism in P2Y1-deficient mice. Nature Med. 5:1199–1202. 1999. View Article : Google Scholar : PubMed/NCBI

16 

Kato K, Ito H, Hasegawa K, Inaguma Y, Kozawa O and Asano T: Modulation of the stress-induced synthesis of hsp27 and α B-crystallin by cyclic AMP in C6 rat glioma cells. J Neurochem. 66:946–950. 1996. View Article : Google Scholar : PubMed/NCBI

17 

Laemmli UK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227:680–685. 1970. View Article : Google Scholar : PubMed/NCBI

18 

Catella F, Healy D, Lawson JA and FitzGerald GA: 11-Dehydrothromboxane B2: a quantitative index of thromboxane A2 formation in the human circulation. Proc Natl Acad Sci USA. 83:5861–5865. 1986. View Article : Google Scholar : PubMed/NCBI

19 

Bertele V, Di Minno G and de Gaetano G: U-46619, a stable analogue of prostaglandin H2, induces retraction of human platelet-rich plasma clots. Thromb Res. 18:543–545. 1980. View Article : Google Scholar : PubMed/NCBI

20 

Gangalum RK, Atanasov IC, Zhou ZH and Bhat SP: αB-crystallin is found in detergent-resistant membrane microdomains and is secreted via exosomes from human retinal pigment epithelial cells. J Biol Chem. 286:3261–3269. 2011. View Article : Google Scholar :

21 

Nakahata N: Thromboxane A2: physiology/pathophysiology, cellular signal transduction and pharmacology. Pharmacol Ther. 118:18–35. 2008. View Article : Google Scholar : PubMed/NCBI

22 

Doi T, Tokud H, Matsushima-Nishiwaki R, Cuong NT, Kageyama Y, Iida Y, Kono A, Akamatsu S, Otsuka T, Iida H, Kozawa O and Ogura S: Effect of antithrombin III on glycoprotein Ib/IX/V activation in human platelets: suppression of thromboxane A2 generation. Prosta Leukot Essent Fatty Acids. 87:57–62. 2012. View Article : Google Scholar

23 

Davi G and Patrono C: Platelet activation and atherothrombosis. New Engl J Med. 357:2482–2494. 2007. View Article : Google Scholar : PubMed/NCBI

24 

Hermann A, Rauch BH, Braun M, Schror K and Weber AA: Platelet CD40 ligand (CD40 L)-subcellular localization, regulation of expression and inhibition by clopidogrel. Platelets. 12:74–82. 2001. View Article : Google Scholar : PubMed/NCBI

25 

Henn V, Slupsky JR, Grafe M, Anagnostopoulos I, Forster R, Muller-Berghaus G, et al: CD40 ligand on activated platelets triggers an inflammatory reaction of endothelial cells. Nature. 391:591–594. 1998. View Article : Google Scholar : PubMed/NCBI

26 

Heeschen C, Dimmeler S, Hamm CW, van den Brand MJ, Boersma E, Zeiher AM, et al: Soluble CD40 ligand in acute coronary syndromes. New Engl J Med. 348:1104–1111. 2003. View Article : Google Scholar : PubMed/NCBI

27 

Varo N, de Lemos JA, Libby P, Morrow DA, Murphy SA, Nuzzo R, et al: Soluble CD40L: risk prediction after acute coronary syndromes. Circulation. 108:1049–1052. 2003. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Tsujimoto M, Doi T, Kuroyanagi G, Yamamoto N, Matsushima‑Nishiwaki R, Iida Y, Enomoto Y, Iida H, Ogura S, Otsuka T, Otsuka T, et al: αB-crystallin reduces ristocetin‑induced soluble CD40 ligand release in human platelets: Suppression of thromboxane A2 generation. Mol Med Rep 12: 357-362, 2015.
APA
Tsujimoto, M., Doi, T., Kuroyanagi, G., Yamamoto, N., Matsushima‑Nishiwaki, R., Iida, Y. ... Iwama, T. (2015). αB-crystallin reduces ristocetin‑induced soluble CD40 ligand release in human platelets: Suppression of thromboxane A2 generation. Molecular Medicine Reports, 12, 357-362. https://doi.org/10.3892/mmr.2015.3445
MLA
Tsujimoto, M., Doi, T., Kuroyanagi, G., Yamamoto, N., Matsushima‑Nishiwaki, R., Iida, Y., Enomoto, Y., Iida, H., Ogura, S., Otsuka, T., Tokuda, H., Kozawa, O., Iwama, T."αB-crystallin reduces ristocetin‑induced soluble CD40 ligand release in human platelets: Suppression of thromboxane A2 generation". Molecular Medicine Reports 12.1 (2015): 357-362.
Chicago
Tsujimoto, M., Doi, T., Kuroyanagi, G., Yamamoto, N., Matsushima‑Nishiwaki, R., Iida, Y., Enomoto, Y., Iida, H., Ogura, S., Otsuka, T., Tokuda, H., Kozawa, O., Iwama, T."αB-crystallin reduces ristocetin‑induced soluble CD40 ligand release in human platelets: Suppression of thromboxane A2 generation". Molecular Medicine Reports 12, no. 1 (2015): 357-362. https://doi.org/10.3892/mmr.2015.3445
Copy and paste a formatted citation
x
Spandidos Publications style
Tsujimoto M, Doi T, Kuroyanagi G, Yamamoto N, Matsushima‑Nishiwaki R, Iida Y, Enomoto Y, Iida H, Ogura S, Otsuka T, Otsuka T, et al: αB-crystallin reduces ristocetin‑induced soluble CD40 ligand release in human platelets: Suppression of thromboxane A2 generation. Mol Med Rep 12: 357-362, 2015.
APA
Tsujimoto, M., Doi, T., Kuroyanagi, G., Yamamoto, N., Matsushima‑Nishiwaki, R., Iida, Y. ... Iwama, T. (2015). αB-crystallin reduces ristocetin‑induced soluble CD40 ligand release in human platelets: Suppression of thromboxane A2 generation. Molecular Medicine Reports, 12, 357-362. https://doi.org/10.3892/mmr.2015.3445
MLA
Tsujimoto, M., Doi, T., Kuroyanagi, G., Yamamoto, N., Matsushima‑Nishiwaki, R., Iida, Y., Enomoto, Y., Iida, H., Ogura, S., Otsuka, T., Tokuda, H., Kozawa, O., Iwama, T."αB-crystallin reduces ristocetin‑induced soluble CD40 ligand release in human platelets: Suppression of thromboxane A2 generation". Molecular Medicine Reports 12.1 (2015): 357-362.
Chicago
Tsujimoto, M., Doi, T., Kuroyanagi, G., Yamamoto, N., Matsushima‑Nishiwaki, R., Iida, Y., Enomoto, Y., Iida, H., Ogura, S., Otsuka, T., Tokuda, H., Kozawa, O., Iwama, T."αB-crystallin reduces ristocetin‑induced soluble CD40 ligand release in human platelets: Suppression of thromboxane A2 generation". Molecular Medicine Reports 12, no. 1 (2015): 357-362. https://doi.org/10.3892/mmr.2015.3445
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