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Article

Enoyl-coenzyme A hydratase short chain 1 silencing attenuates the proliferation of hepatocellular carcinoma by inhibiting epidermal growth factor signaling in vitro and in vivo

  • Authors:
    • Bi-Yun Lin
    • Chuan-Xing Xiao
    • Wen-Xiu Zhao
    • Li Xiao
    • Xu Chen
    • Ping Li
    • Xiao-Min Wang
  • View Affiliations / Copyright

    Affiliations: Fujian Provincial Key Laboratory of Chronic Liver Disease and Hepatocellular Carcinoma, Department of Hepatobiliary Surgery, Zhongshan Hospital Affiliated to Xiamen University, Xiamen, Fujian 361004, P.R. China, Department of Gastroenterology, Zhongshan Hospital Affiliated to Xiamen University, Xiamen, Fujian 361004, P.R. China
  • Pages: 1421-1428
    |
    Published online on: March 9, 2015
       https://doi.org/10.3892/mmr.2015.3453
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Abstract

Enoyl-coenzyme A hydratase short chain 1 (ECHS1) regulates fatty acid metabolism and is an essential factor in tumor development. The present study aimed to investigate the molecular mechanisms of ECHS1 in hepatocellular carcinogenesis by studying proliferation and survival in ECHS1 knocked-down hepatocellular carcinoma (HCC) cell lines, HepG2 and HuH7. The effect of ECHS1 on tumor development was investigated by tumor transplantation in nude mice, and the signaling pathways involved in the ECHS1-mediated regulation of HCC cell proliferation were identified by western blot analysis. The silencing of ECHS1 suppressed HCC cell proliferation in vitro and suppressed the growth of transplanted tumors in vivo. In addition, the phosphorylation of EGFR and its downstream effectors ERK1/2 and AKT was downregulated in ECHS1 knocked-down cells and tumor tissues. Furthermore, knockdown of ECHS1 in HCC suppressed cyclin D3 and cyclin dependent kinase 6 expression, whilst enhancing p16 and p21 expression. Therefore, ECHS1 may also be involved in cell cycle progression in HCC cells. These results suggested that ECHS1 may promote cell proliferation in HCC in an EGFR-dependent manner.
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Copy and paste a formatted citation
Spandidos Publications style
Lin B, Xiao C, Zhao W, Xiao L, Chen X, Li P and Wang X: Enoyl-coenzyme A hydratase short chain 1 silencing attenuates the proliferation of hepatocellular carcinoma by inhibiting epidermal growth factor signaling in vitro and in vivo. Mol Med Rep 12: 1421-1428, 2015.
APA
Lin, B., Xiao, C., Zhao, W., Xiao, L., Chen, X., Li, P., & Wang, X. (2015). Enoyl-coenzyme A hydratase short chain 1 silencing attenuates the proliferation of hepatocellular carcinoma by inhibiting epidermal growth factor signaling in vitro and in vivo. Molecular Medicine Reports, 12, 1421-1428. https://doi.org/10.3892/mmr.2015.3453
MLA
Lin, B., Xiao, C., Zhao, W., Xiao, L., Chen, X., Li, P., Wang, X."Enoyl-coenzyme A hydratase short chain 1 silencing attenuates the proliferation of hepatocellular carcinoma by inhibiting epidermal growth factor signaling in vitro and in vivo". Molecular Medicine Reports 12.1 (2015): 1421-1428.
Chicago
Lin, B., Xiao, C., Zhao, W., Xiao, L., Chen, X., Li, P., Wang, X."Enoyl-coenzyme A hydratase short chain 1 silencing attenuates the proliferation of hepatocellular carcinoma by inhibiting epidermal growth factor signaling in vitro and in vivo". Molecular Medicine Reports 12, no. 1 (2015): 1421-1428. https://doi.org/10.3892/mmr.2015.3453
Copy and paste a formatted citation
x
Spandidos Publications style
Lin B, Xiao C, Zhao W, Xiao L, Chen X, Li P and Wang X: Enoyl-coenzyme A hydratase short chain 1 silencing attenuates the proliferation of hepatocellular carcinoma by inhibiting epidermal growth factor signaling in vitro and in vivo. Mol Med Rep 12: 1421-1428, 2015.
APA
Lin, B., Xiao, C., Zhao, W., Xiao, L., Chen, X., Li, P., & Wang, X. (2015). Enoyl-coenzyme A hydratase short chain 1 silencing attenuates the proliferation of hepatocellular carcinoma by inhibiting epidermal growth factor signaling in vitro and in vivo. Molecular Medicine Reports, 12, 1421-1428. https://doi.org/10.3892/mmr.2015.3453
MLA
Lin, B., Xiao, C., Zhao, W., Xiao, L., Chen, X., Li, P., Wang, X."Enoyl-coenzyme A hydratase short chain 1 silencing attenuates the proliferation of hepatocellular carcinoma by inhibiting epidermal growth factor signaling in vitro and in vivo". Molecular Medicine Reports 12.1 (2015): 1421-1428.
Chicago
Lin, B., Xiao, C., Zhao, W., Xiao, L., Chen, X., Li, P., Wang, X."Enoyl-coenzyme A hydratase short chain 1 silencing attenuates the proliferation of hepatocellular carcinoma by inhibiting epidermal growth factor signaling in vitro and in vivo". Molecular Medicine Reports 12, no. 1 (2015): 1421-1428. https://doi.org/10.3892/mmr.2015.3453
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