Zanthoxylum schinifolium leaf ethanol extract inhibits adipocyte differentiation through inactivation of the extracellular signal regulated kinase and phosphoinositide 3-kinase/Akt signaling pathways in 3T3-L1 pre-adipocytes

Choi,Eun‑Ok; Park,Cheol; Shin,Soon  Shik; Cho,Eun‑Ju; Kim,Byung  Woo; Hwang,Jin  Ah; Hwang,Hye‑Jin; Choi,Yung  Hyun

doi:10.3892/mmr.2015.3463

Zanthoxylum schinifolium leaf ethanol extract inhibits adipocyte differentiation through inactivation of the extracellular signal regulated kinase and phosphoinositide 3-kinase/Akt signaling pathways in 3T3-L1 pre-adipocytes

Authors:
- Eun‑Ok Choi
- Cheol Park
- Soon Shik Shin
- Eun‑Ju Cho
- Byung Woo Kim
- Jin Ah Hwang
- Hye‑Jin Hwang
- Yung Hyun Choi
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Affiliations: Department of Food and Nutrition, Busan National University, Busan 609‑735, Republic of Korea, Department of Molecular Biology, Dongeui University, Busan 614‑714, Republic of Korea, Department of Formula Sciences, Dongeui University College of Korean Medicine, Busan 614‑052, Republic of Korea, Department of Life Science and Biotechnology, Dongeui University, Busan 614‑714, Republic of Korea, Department of Food and Nutrition, Myongji University, Yongin 449‑728, Republic of Korea, Anti‑Aging Research Center, Dongeui University, Busan 614‑714, Republic of Korea
Published online on: March 10, 2015 https://doi.org/10.3892/mmr.2015.3463
Pages: 1314-1320

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Abstract

Zanthoxylum schinifolium is widely used as a food flavoring in east Asia. Although this plant has also been used in traditional oriental medicine for the treatment of the common cold, toothache, stomach ache, diarrhea and jaundice, its anti‑obesity activity remains to be elucidated. The present study investigated the effects of ethanol extract from the leaves of Z. schinifolium (EEZS) on adipocyte differentiation, and its underlying mechanism, in 3T3‑L1 pre‑adipocytes. The results demonstrated that EEZS effectively suppressed intracellular lipid accumulation at non‑toxic concentrations, and was associated with the downregulation of several adipocyte‑specific transcription factors, including peroxisome proliferation‑activity receptor γ (PPARγ), CCAAT/enhancer binding protein (C/EBP)α and C/EBPβ, in a concentration‑dependent manner. Furthermore, it was observed that EEZS markedly inactivated the extracellular signal‑regulated protein kinase (ERK) and phosphatidylinositide 3‑kinase (PI3K)/Akt pathways, which act upstream of PPARγ and C/EBPs in adipogenesis. These results suggested that EEZS inhibited lipid accumulation by downregulating the major transcription factors involved in the pathway of adipogenesis, including PPARγ, C/EBPα and C/EBPβ, via regulation of the ERK and PI3K/Akt signaling pathways in 3T3‑L1 adipocyte differentiation. This indicated the potential use of EEZS as an anti‑obesity agent.

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