Curcuma zedoaria (Berg.) Rosc. essential oil and paclitaxel synergistically enhance the apoptosis of SKOV3 cells

Zhou,Yunxiao; Shen,Jie; Xia,Liqun; Wang,Yanli

doi:10.3892/mmr.2015.3473

Curcuma zedoaria (Berg.) Rosc. essential oil and paclitaxel synergistically enhance the apoptosis of SKOV3 cells

Authors:
- Yunxiao Zhou
- Jie Shen
- Liqun Xia
- Yanli Wang
View Affiliations

Affiliations: Department of Gynecology, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang 310003, P.R. China, Department of Pathology, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang 310003, P.R. China
Published online on: March 11, 2015 https://doi.org/10.3892/mmr.2015.3473
Pages: 1253-1257

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

Curcuma zedoaria (Berg.) Rosc. essential oil (CZEO) is the major component of Curcuma zedoaria (Berg.) Rosc., a traditional medicine with antitumor activity. Paclitaxel (PTX) is a first‑line chemotherapeutic agent used to treat patients with ovarian cancer. These compounds directly target nuclear DNA, in order to suppress or inhibit tumor cell growth. The present study aimed to determine the synergistic antitumor effects of CZEO and PTX on the SKOV3 human ovarian cancer cell line. SKOV3 cells were treated with CZEO, PTX or a combination of the two and cell viability was detected using cell counting kit‑8. In addition, flow cytometry was used to determined cell apoptosis as well as for cell cycle analysis. The morphological changes of apoptosis were assessed using Hoechst 33342 staining and the expression levels of apoptotic pathway proteins, including caspase‑3 and poly (ADP‑ribose) polymerase (PARP), were quantified using western blot analysis. The cell viability assay indicated that either of these compounds alone or in combination suppressed the growth of SKOV3 cells. Furthermore, flow cytometric analysis indicated that treatment with a combination of CZEO and PTX resulted in increased inhibition of proliferation and induction of apoptosis of SKOV3 cells, as compared with treatment with either of the compounds alone. In addition, the protein expression levels of caspase‑3 were increased following treatment with a combination of CZEO and PTX. The results of the present study suggested that CZEO and PTX synergistically enhanced the inhibition of SKOV3 proliferation, and the possible underlying mechanism may be the induction of cell apoptosis and cell cycle arrest. This therefore indicated that PTX supplemented with CZEO may be an effective treatment strategy to decrease the dose and toxicity of PTX.

View Figures

View References

1	Blagosklonny MV and Fojo T: Molecular effects of paclitaxel: myths and reality (a critical review). Int J Cancer. 83:151–156. 1999. View Article : Google Scholar : PubMed/NCBI
2	Neijt JP, Engelholm SA, Tuxen MK, et al: Exploratory phase III study of paclitaxel and cisplatin versus paclitaxel and carboplatin in advanced ovarian cancer. J Clin Oncol. 18:3084–3092. 2000.PubMed/NCBI
3	Chen W, Lu Y, Gao M, Wu J, Wang A and Shi R: Anti-angiogenesis effect of essential oil from Curcuma zedoaria in vitro and in vivo. J Ethnopharmacol. 133:220–226. 2011. View Article : Google Scholar
4	Lobo R, Prabhu KS and Shirwaikar A and Shirwaikar A: Curcuma zedoaria Rosc. (white turmeric): a review of its chemical, pharmacological and ethnomedicinal properties. J Pharm Pharmacol. 61:13–21. 2009. View Article : Google Scholar : PubMed/NCBI
5	Lakshmi S, Padmaja G and Remani P: Antitumour effects of isocurcumenol isolated from Curcuma zedoaria rhizomes on human and murine cancer cells. International Journal of Medicinal Chemistry. 2011:20112011. View Article : Google Scholar
6	Kim KI, Kim JW, Hong BS, et al: Antitumor, genotoxicity and anticlastogenic activities of polysaccharide from Curcuma zedoaria. Mol Cells. 10:392–398. 2000.PubMed/NCBI
7	Seo WG, Hwang JC, Kang SK, et al: Suppressive effect of Zedoariae rhizoma on pulmonary metastasis of B16 melanoma cells. J Ethnopharmacol. 101:249–257. 2005. View Article : Google Scholar : PubMed/NCBI
8	Shin Y and Lee Y: Cytotoxic activity from Curcuma zedoaria through mitochondrial activation on ovarian cancer cells. Toxicol Res. 29:257–261. 2013. View Article : Google Scholar
9	Wu WY, Luo YJ and Cheng JH: Zedoary turmeric oil inhibits tranplantal hepatoma in rat via hepatic artery perfusion. Shi Jie Hua Ren Xiao Hua Za Zhi. 11:260–263. 1998.
10	Giampietri A, Bonmassar A, Puccetti P, et al: Drug-mediated increase of tumor immunogenicity in vivo for a new approach to experimental cancer immunotherapy. Cancer Res. 41:681–687. 1981.PubMed/NCBI
11	Elmore S: Apoptosis: a review of programmed cell death. Toxicol Pathol. 35:495–516. 2007. View Article : Google Scholar : PubMed/NCBI
12	Jiang C, Wang Z, Ganther H, et al: Caspases as key executors of methyl selenium-induced apoptosis (anoikis) of DU-145 prostate cancer cells. Cancer Res. 61:3062–3070. 2001.PubMed/NCBI
13	Lakhani SA, Masud A, Kuida K, et al: Caspases 3 and 7: key mediators of mitochondrial events of apoptosis. Science. 311:847–851. 2006. View Article : Google Scholar : PubMed/NCBI
14	Chowdhry MF, Vohra HA and Galiñanes M: Diabetes increases apoptosis and necrosis in both ischemic and nonischemic human myocardium: Role of caspases and poly–adenosine diphosphate–ribose polymerase. J Thorac Cardiovasc Surg. 134:124–131. 2007. View Article : Google Scholar
15	Chen CC, Chen Y, Hsi YT, et al: Chemical constituents and anticancer activity of Curcuma zedoaria roscoe essential oil against non-small cell lung carcinoma cells in vitro and in vivo. J Agric Food Chem. 61:11418–11427. 2013. View Article : Google Scholar : PubMed/NCBI
16	Chou TC and Talalay P: Quantitative analysis of dose-effect relationships: the combined effects of multiple drugs or enzyme inhibitors. Adv Enzyme Regul. 22:27–55. 1984. View Article : Google Scholar : PubMed/NCBI
17	Smith RA, Brooks D, Cokkinides V, et al: Cancer screening in the United States, 2013: a review of current American Cancer Society guidelines, current issues in cancer screening, and new guidance on cervical cancer screening and lung cancer screening. CA Cancer J Clin. 63:87–105. 2013. View Article : Google Scholar
18	McGuire V, Jesser CA and Whittemore AS: Survival among U.S. woman with invasive epithelical ovarian cancer. Gynecol Oncol. 84:399–403. 2002. View Article : Google Scholar : PubMed/NCBI
19	Hossein G, Keshavarz M, Ahmadi S and Naderi N: Synergistic effects of PectaSol-C modified citrus pectin an inhibitor of Galectin-3 and paclitaxel on apoptosis of human SKOV-3 ovarian cancer cells. Asian Pac J Cancer Prev. 14:7561–7568. 2013. View Article : Google Scholar
20	Kushner DM, Connor JP, Sanchez F, et al Wisconsin Oncology Network: Weekly docetaxel and carboplatin for recurrent ovarian and peritoneal cancer: a phase II trial. Gynecol Oncol. 105:358–364. 2007. View Article : Google Scholar : PubMed/NCBI
21	Wani MC, Taylor HL, Wall ME, Coggon P and McPhail AT: Plant antitumor agents. VI. The isolation and structure of taxol, a novel antileukemic and antitumor agent from Taxus brevifolia. J Am Chem Soc. 93:2325–2357. 1971. View Article : Google Scholar : PubMed/NCBI
22	Maier-Lenz H, Hauns B, Haering B, et al: Phase I study of paclitaxel administered as a 1-hour infusion: toxicity and pharmacokinetics. Semin Oncol. 24:S16–S19. 1997.
23	Scripture CD, Figg WD and Sparreboom A: Peripheral neuropathy induced by paclitaxel: recent insights and future perspectives. Curr Neuropharmacol. 4:165–172. 2006. View Article : Google Scholar
24	Cang S, Ma Y, Chiao JW and Liu D: Phenethyl isothiocyanate and paclitaxel synergistically enhanced apoptosis and alpha-tubulin hyperacetylation in breast cancer cells. Exp Hematol Oncol. 3:52014. View Article : Google Scholar :
25	Yang MY, Wang CJ, Chen NF, Ho WH, Lu FJ and Tseng TH: Luteolin enhances paclitaxel-induced apoptosis in human breast cancer MDA-MB-231 cells by blocking STAT3. Chem Biol Interact. 213:60–68. 2014. View Article : Google Scholar : PubMed/NCBI
26	Kang H, Lee SH, Price JE and Kim LS: Curcumin suppresses the paclitaxel-induced nuclear factor-kappaB in breast cancer cells and potentiates the growth inhibitory effect of paclitaxel in a breast cancer nude mice model. Breast J. 15:223–229. 2009. View Article : Google Scholar : PubMed/NCBI
27	Kuo HC, Lee HJ, Hu CC, Shun HI and Tseng TH: Enhancement of esculetin on Taxol-induced apoptosis in human hepatoma HepG2 cells. Toxicol Appl Pharmacol. 210:55–62. 2006. View Article : Google Scholar
28	Long KJ, Han FJ, Wu XK and Wang XY: Effects of Zedoary turmeric oil injection on SKOV3 cell proliferation and pathological morphological changes in ovarian cancer. World Journal of Integrated Traditional and Western Medicine. 6:660–662. 2011.
29	George J, Banik NL and Ray SK: Molecular Mechanisms of Taxol for Induction of Cell Death in Glioblastomas. Glioblastoma: Molecular Mechanisms of Pathogenesis and Current Therapeutic Strategies. Ray SK: Springer; New York: pp. 283–298. 2010, View Article : Google Scholar
30	Gonçalves A, Braguer D, Carles G, André N, Prevôt C and Briand C: Caspase-8 activation independent of CD95/CD95-L interaction during paclitaxel-induced apoptosis in human colon cancer cells (HT29-D4). Biochem Pharmacol. 60:1579–1584. 2000. View Article : Google Scholar : PubMed/NCBI
31	Perkins C, Kim CN, Fang G and Bhalla KN: Overexpression of Apaf-1 promotes apoptosis of untreated and paclitaxel- or etoposide-treated HL-60 cells. Cancer Res. 58:4561–4566. 1998.PubMed/NCBI
32	Weigel TL, Lotze MT, Kim PK, Amoscato AA, Luketich JD and Odoux C: Paclitaxel-induced apoptosis in non-small cell lung cancer cell lines is associated with increased caspase-3 activity. J Thorac Cardiovasc Surg. 119:795–803. 2000. View Article : Google Scholar : PubMed/NCBI