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Article

Apoptosis induced by sonodynamic therapy in human osteosarcoma cells in vitro

  • Authors:
    • Xing Liu
    • Wei Li
    • Shuo Geng
    • Qing‑Gang Meng
    • Zheng‑Gang Bi
  • View Affiliations / Copyright

    Affiliations: Department of Orthopaedic Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China, Department of Orthopedic Surgery, The First Hospital of Harbin City, Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China
  • Pages: 1183-1188
    |
    Published online on: March 12, 2015
       https://doi.org/10.3892/mmr.2015.3479
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Abstract

The aim of the present study was to investigate the potential effect of hematoporphyrin monomethyl ether-sonodynamic therapy (HMME‑SDT) on MG‑63 osteosarcoma cells. The HMME concentration was kept constant at 20 µg/ml and the MG‑63 osteosarcoma cell line was exposed to ultrasound with an intensity of 1.0 W/cm2 for 30 sec. Cell cytotoxicity was quantified using an MTT assay 6 h after HMME‑SDT. The intracellular localization of HMME was imaged using inverted confocal laser scanning microscopy. Apoptosis was investigated using flow cytometry with Annexin V‑fluorescein isothiocyanate and propidium iodine staining. The cytotoxicity of HMME‑mediated sonodynamic action on MG‑63 cells was significantly higher than that of other treatments, including ultrasound alone, HMME alone and sham treatment. Flow cytometry demonstrated that HMME‑SDT action markedly enhanced the apoptotic rate of MG‑63 cells. The mechanisms of apoptosis were analyzed by measuring the protein expression of poly ADP‑ribose polymerase (PARP), cleaved PARP, procaspase‑3, cleaved caspase‑3 and cleaved caspase‑9. The data demonstrated that HMME‑SDT action markedly induced the apoptosis of MG‑63 cells.
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Copy and paste a formatted citation
Spandidos Publications style
Liu X, Li W, Geng S, Meng QG and Bi ZG: Apoptosis induced by sonodynamic therapy in human osteosarcoma cells in vitro. Mol Med Rep 12: 1183-1188, 2015.
APA
Liu, X., Li, W., Geng, S., Meng, Q., & Bi, Z. (2015). Apoptosis induced by sonodynamic therapy in human osteosarcoma cells in vitro. Molecular Medicine Reports, 12, 1183-1188. https://doi.org/10.3892/mmr.2015.3479
MLA
Liu, X., Li, W., Geng, S., Meng, Q., Bi, Z."Apoptosis induced by sonodynamic therapy in human osteosarcoma cells in vitro". Molecular Medicine Reports 12.1 (2015): 1183-1188.
Chicago
Liu, X., Li, W., Geng, S., Meng, Q., Bi, Z."Apoptosis induced by sonodynamic therapy in human osteosarcoma cells in vitro". Molecular Medicine Reports 12, no. 1 (2015): 1183-1188. https://doi.org/10.3892/mmr.2015.3479
Copy and paste a formatted citation
x
Spandidos Publications style
Liu X, Li W, Geng S, Meng QG and Bi ZG: Apoptosis induced by sonodynamic therapy in human osteosarcoma cells in vitro. Mol Med Rep 12: 1183-1188, 2015.
APA
Liu, X., Li, W., Geng, S., Meng, Q., & Bi, Z. (2015). Apoptosis induced by sonodynamic therapy in human osteosarcoma cells in vitro. Molecular Medicine Reports, 12, 1183-1188. https://doi.org/10.3892/mmr.2015.3479
MLA
Liu, X., Li, W., Geng, S., Meng, Q., Bi, Z."Apoptosis induced by sonodynamic therapy in human osteosarcoma cells in vitro". Molecular Medicine Reports 12.1 (2015): 1183-1188.
Chicago
Liu, X., Li, W., Geng, S., Meng, Q., Bi, Z."Apoptosis induced by sonodynamic therapy in human osteosarcoma cells in vitro". Molecular Medicine Reports 12, no. 1 (2015): 1183-1188. https://doi.org/10.3892/mmr.2015.3479
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