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Article

MicroRNA-21 promotes osteogenic differentiation by targeting small mothers against decapentaplegic 7

  • Authors:
    • Hongqiu Li
    • Feng Yang
    • Zhe Wang
    • Qin Fu
    • A Liang
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, Shengjing Hospital of China Medical University, Shenyang 110004, P.R. China, Department of Radiology, Central Hospital of Shenyang Medical College, Shenyang 110024, P.R. China, Department of Blood Transfusion, Shengjing Hospital of China Medical University, Shenyang, Liaoning 110004, P.R. China, Department of Orthopedics, Central Hospital of Shenyang Medical College, Shenyang 110024, P.R. China
  • Pages: 1561-1567
    |
    Published online on: March 17, 2015
       https://doi.org/10.3892/mmr.2015.3497
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Abstract

Previous studies have suggested that microRNAs (miRNAs/miRs) may positively or negatively control osteogenic differentiation and mineralization by targeting negative regulators of osteogenesis or important osteogenic factors. miR‑21 is important in osteoblast differentiation and Smad7 is a critical regulator of osteogenic differentiation, which inhibits proliferation, differentiation and mineralization in mouse osteoblast cells. However, the association between Smad7 and miR‑21 remain to be elucidated. In the present study, miR‑21 was found to promote the level of osteogenic differentiation and increase matrix mineralization in MC3T3‑E1 cells. Furthermore, Smad7 was identified as a direct target of miR‑21 in the MC3T3‑E1 cells. The overexpression of miR‑21 affected the protein levels of SMAD7, but not the mRNA levels, which suggested that miR‑21 regulates the levels of SMAD7 by inhibiting translation, rather than by promoting mRNA decay. Forced expression of miR‑21 promoted osteogenic differentiation and mineralization, while inhibition of miR‑21 suppressed these processes. The present study also identified for the first time, to the best of our knowledge, the promotion of osteogenic differentiation and mineralization by miR‑21, by repressing the expression of Smad7.
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Copy and paste a formatted citation
Spandidos Publications style
Li H, Yang F, Wang Z, Fu Q and Liang A: MicroRNA-21 promotes osteogenic differentiation by targeting small mothers against decapentaplegic 7. Mol Med Rep 12: 1561-1567, 2015.
APA
Li, H., Yang, F., Wang, Z., Fu, Q., & Liang, A. (2015). MicroRNA-21 promotes osteogenic differentiation by targeting small mothers against decapentaplegic 7. Molecular Medicine Reports, 12, 1561-1567. https://doi.org/10.3892/mmr.2015.3497
MLA
Li, H., Yang, F., Wang, Z., Fu, Q., Liang, A."MicroRNA-21 promotes osteogenic differentiation by targeting small mothers against decapentaplegic 7". Molecular Medicine Reports 12.1 (2015): 1561-1567.
Chicago
Li, H., Yang, F., Wang, Z., Fu, Q., Liang, A."MicroRNA-21 promotes osteogenic differentiation by targeting small mothers against decapentaplegic 7". Molecular Medicine Reports 12, no. 1 (2015): 1561-1567. https://doi.org/10.3892/mmr.2015.3497
Copy and paste a formatted citation
x
Spandidos Publications style
Li H, Yang F, Wang Z, Fu Q and Liang A: MicroRNA-21 promotes osteogenic differentiation by targeting small mothers against decapentaplegic 7. Mol Med Rep 12: 1561-1567, 2015.
APA
Li, H., Yang, F., Wang, Z., Fu, Q., & Liang, A. (2015). MicroRNA-21 promotes osteogenic differentiation by targeting small mothers against decapentaplegic 7. Molecular Medicine Reports, 12, 1561-1567. https://doi.org/10.3892/mmr.2015.3497
MLA
Li, H., Yang, F., Wang, Z., Fu, Q., Liang, A."MicroRNA-21 promotes osteogenic differentiation by targeting small mothers against decapentaplegic 7". Molecular Medicine Reports 12.1 (2015): 1561-1567.
Chicago
Li, H., Yang, F., Wang, Z., Fu, Q., Liang, A."MicroRNA-21 promotes osteogenic differentiation by targeting small mothers against decapentaplegic 7". Molecular Medicine Reports 12, no. 1 (2015): 1561-1567. https://doi.org/10.3892/mmr.2015.3497
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