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Article

Vaccarin promotes endothelial cell proliferation in association with neovascularization in vitro and in vivo

  • Authors:
    • Fengshan Xie
    • Lei Feng
    • Weiwei Cai
    • Yuyu Qiu
    • Yanling  Liu
    • Yue Li
    • Bin Du
    • Liying Qiu
  • View Affiliations / Copyright

    Affiliations: Laboratory of Natural Medicine, School of Pharmaceutical Science, Jiangnan University, Wuxi, Jiangsu 214122, P.R. China, Wuxi Medical School, Jiangnan University, Wuxi, Jiangsu 214122, P.R. China
  • Pages: 1131-1136
    |
    Published online on: March 18, 2015
       https://doi.org/10.3892/mmr.2015.3503
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Abstract

Angiogenesis is a major pathological component of several diseases, including traumatic vascular disease and coronary heart disease. The purpose of the present study was to determine the effects of vaccarin on endothelial cell migration and neovascularization, which are important and necessary components of wound healing. The present study investigated and confirmed neovascularization induced by vaccarin in vitro and in vivo. In vitro, the effects of vaccarin (1.08 and 2.15 µM) on proliferation, migration and tube formation of human microvascular endothelial cells (HMEC)‑1 were evaluated via sulforhodamine B assay and migration and tube formation assay, respectively. Furthermore, a mouse Matrigel plus model was used to detect capillary‑like tube structures in vivo. Immunohistochemistry was used to detect the protein expression of cluster of differentiation 31 (CD31), p‑AKT and p‑extracellular‑signal‑regulated kinases (Erk). Vaccarin significantly promoted HMEC‑1 proliferation and migration and tube formation of HMEC‑1 at a dose of 2.15 µM. In vivo, vaccarin delivered by daily oral administration significantly improved epidermal growth factor‑induced angiogenesis in an intradermal inoculation mouse model. The mouse Matrigel model study also revealed that vaccarin significantly promoted neovascularization via detection of CD31 levels and enhanced protein expression of p‑Akt and p‑Erk. In addition, vaccarin also promoted expression of CD31.
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Copy and paste a formatted citation
Spandidos Publications style
Xie F, Feng L, Cai W, Qiu Y, Liu Y, Li Y, Du B and Qiu L: Vaccarin promotes endothelial cell proliferation in association with neovascularization in vitro and in vivo. Mol Med Rep 12: 1131-1136, 2015.
APA
Xie, F., Feng, L., Cai, W., Qiu, Y., Liu, Y., Li, Y. ... Qiu, L. (2015). Vaccarin promotes endothelial cell proliferation in association with neovascularization in vitro and in vivo. Molecular Medicine Reports, 12, 1131-1136. https://doi.org/10.3892/mmr.2015.3503
MLA
Xie, F., Feng, L., Cai, W., Qiu, Y., Liu, Y., Li, Y., Du, B., Qiu, L."Vaccarin promotes endothelial cell proliferation in association with neovascularization in vitro and in vivo". Molecular Medicine Reports 12.1 (2015): 1131-1136.
Chicago
Xie, F., Feng, L., Cai, W., Qiu, Y., Liu, Y., Li, Y., Du, B., Qiu, L."Vaccarin promotes endothelial cell proliferation in association with neovascularization in vitro and in vivo". Molecular Medicine Reports 12, no. 1 (2015): 1131-1136. https://doi.org/10.3892/mmr.2015.3503
Copy and paste a formatted citation
x
Spandidos Publications style
Xie F, Feng L, Cai W, Qiu Y, Liu Y, Li Y, Du B and Qiu L: Vaccarin promotes endothelial cell proliferation in association with neovascularization in vitro and in vivo. Mol Med Rep 12: 1131-1136, 2015.
APA
Xie, F., Feng, L., Cai, W., Qiu, Y., Liu, Y., Li, Y. ... Qiu, L. (2015). Vaccarin promotes endothelial cell proliferation in association with neovascularization in vitro and in vivo. Molecular Medicine Reports, 12, 1131-1136. https://doi.org/10.3892/mmr.2015.3503
MLA
Xie, F., Feng, L., Cai, W., Qiu, Y., Liu, Y., Li, Y., Du, B., Qiu, L."Vaccarin promotes endothelial cell proliferation in association with neovascularization in vitro and in vivo". Molecular Medicine Reports 12.1 (2015): 1131-1136.
Chicago
Xie, F., Feng, L., Cai, W., Qiu, Y., Liu, Y., Li, Y., Du, B., Qiu, L."Vaccarin promotes endothelial cell proliferation in association with neovascularization in vitro and in vivo". Molecular Medicine Reports 12, no. 1 (2015): 1131-1136. https://doi.org/10.3892/mmr.2015.3503
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