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Litsea japonica extract inhibits neuronal apoptosis and the accumulation of advanced glycation end products in the diabetic mouse retina

  • Authors:
    • Junghyun Kim
    • Chan‑Sik Kim
    • Yun Mi Lee
    • Eunjin Sohn
    • Kyuhyung Jo
    • Jin Sook Kim
  • View Affiliations / Copyright

    Affiliations: Korean Medicine Based Herbal Drug Development Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine, Daejeon 305‑811, Republic of Korea
    Copyright: © Kim et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 1075-1081
    |
    Published online on: March 24, 2015
       https://doi.org/10.3892/mmr.2015.3543
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Abstract

The retinal accumulation of advanced glycation end products (AGEs) is a condition, which is found in diabetic retinopathy. The purpose of the present study was to investigate the protective effect of Litsea japonica extract (LJE) and to elucidate its underlying protective mechanism in model diabetic db/db mice. Male, 7 ‑week‑old db/db mice were treated with LJE (100 or 250 mg/kg body weight) once a day orally for 12 weeks. The expression levels of AGEs and their receptor (RAGE) were subsequently assessed by immunohistochemistry. An electrophoretic mobility shift assay and southwestern histochemistry were used to detect activated nuclear factor κB (NF‑κB). The immunohistochemical analysis demonstrated that LJE significantly reduced the expression levels of the AGEs and RAGE in the neural retinas of the db/db mice. LJE markedly inhibited the apoptosis of retinal ganglion cells. In addition, LJE suppressed the activation of NF‑κB. These results suggested that LJE may be beneficial for the treatment of diabetes‑induced retinal neurodegeneration, and the ability of LJE to attenuate retinal ganglion cell loss may be mediated by inhibition of the accumulation of AGEs.
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Copy and paste a formatted citation
Spandidos Publications style
Kim J, Kim CS, Lee YM, Sohn E, Jo K and Kim JS: Litsea japonica extract inhibits neuronal apoptosis and the accumulation of advanced glycation end products in the diabetic mouse retina. Mol Med Rep 12: 1075-1081, 2015.
APA
Kim, J., Kim, C., Lee, Y.M., Sohn, E., Jo, K., & Kim, J.S. (2015). Litsea japonica extract inhibits neuronal apoptosis and the accumulation of advanced glycation end products in the diabetic mouse retina. Molecular Medicine Reports, 12, 1075-1081. https://doi.org/10.3892/mmr.2015.3543
MLA
Kim, J., Kim, C., Lee, Y. M., Sohn, E., Jo, K., Kim, J. S."Litsea japonica extract inhibits neuronal apoptosis and the accumulation of advanced glycation end products in the diabetic mouse retina". Molecular Medicine Reports 12.1 (2015): 1075-1081.
Chicago
Kim, J., Kim, C., Lee, Y. M., Sohn, E., Jo, K., Kim, J. S."Litsea japonica extract inhibits neuronal apoptosis and the accumulation of advanced glycation end products in the diabetic mouse retina". Molecular Medicine Reports 12, no. 1 (2015): 1075-1081. https://doi.org/10.3892/mmr.2015.3543
Copy and paste a formatted citation
x
Spandidos Publications style
Kim J, Kim CS, Lee YM, Sohn E, Jo K and Kim JS: Litsea japonica extract inhibits neuronal apoptosis and the accumulation of advanced glycation end products in the diabetic mouse retina. Mol Med Rep 12: 1075-1081, 2015.
APA
Kim, J., Kim, C., Lee, Y.M., Sohn, E., Jo, K., & Kim, J.S. (2015). Litsea japonica extract inhibits neuronal apoptosis and the accumulation of advanced glycation end products in the diabetic mouse retina. Molecular Medicine Reports, 12, 1075-1081. https://doi.org/10.3892/mmr.2015.3543
MLA
Kim, J., Kim, C., Lee, Y. M., Sohn, E., Jo, K., Kim, J. S."Litsea japonica extract inhibits neuronal apoptosis and the accumulation of advanced glycation end products in the diabetic mouse retina". Molecular Medicine Reports 12.1 (2015): 1075-1081.
Chicago
Kim, J., Kim, C., Lee, Y. M., Sohn, E., Jo, K., Kim, J. S."Litsea japonica extract inhibits neuronal apoptosis and the accumulation of advanced glycation end products in the diabetic mouse retina". Molecular Medicine Reports 12, no. 1 (2015): 1075-1081. https://doi.org/10.3892/mmr.2015.3543
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