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Article

Effects of cinobufacini injection on cell proliferation and the expression of topoisomerases in human HepG‑2 hepatocellular carcinoma cells

  • Authors:
    • Ying Liu
    • Li‑Ying Ban
    • Xi Su
    • Shan Gao
    • Ji‑Wei Liu
    • Xiao‑Nan Cui
  • View Affiliations / Copyright

    Affiliations: Department of Oncology, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116011, P.R. China
  • Pages: 1598-1604
    |
    Published online on: March 26, 2015
       https://doi.org/10.3892/mmr.2015.3552
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Abstract

The present study aimed to investigate the effects of cinobufacini injection on the proliferation and expression of topoisomerases in human HepG‑2 hepatocarcinoma cells. The cells were divided into a control group and an experimental group, in which 0.105, 0.21, 0.42 mg/l cinobufacini was injected. Cell proliferation was assessed using a 3‑(4,5‑dimethylthiazol‑2‑yl) ‑2,5‑diphenyltetrazolium bromide assay, levels of apoptosis were detected using annexin V/propidium iodide staining and cell cycles were analyzed using flow cytometric analysis. The mRNA and protein expression levels of topoisomerase (TOPO) I and TOPO Ⅱ were determined by reverse transcription‑quantitative polymerase chain reaction and western blotting, respectively. Cinobufacini injection significantly inhibited the proliferation of the HepG‑2 cells (P<0.05), induced apoptosis (P<0.05) in a dose‑ and time‑dependent manner, induced tumor cell arrest at the S phase in a dose‑dependent manner, and downregulated the mRNA and protein expression levels of TOPO I and TOPO Ⅱ (P<0.05) in a dose‑dependent manner. Therefore, cinobufacini was found to inhibit human HepG‑2 hepatocellular carcinoma cell proliferation, and downregulation of the expression levels of TOPO I and TOPO Ⅱ may contribute to the effect on proliferation observed in the Hep‑G2 cells following cinobbufacini injection.
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Copy and paste a formatted citation
Spandidos Publications style
Liu Y, Ban LY, Su X, Gao S, Liu JW and Cui XN: Effects of cinobufacini injection on cell proliferation and the expression of topoisomerases in human HepG‑2 hepatocellular carcinoma cells. Mol Med Rep 12: 1598-1604, 2015.
APA
Liu, Y., Ban, L., Su, X., Gao, S., Liu, J., & Cui, X. (2015). Effects of cinobufacini injection on cell proliferation and the expression of topoisomerases in human HepG‑2 hepatocellular carcinoma cells. Molecular Medicine Reports, 12, 1598-1604. https://doi.org/10.3892/mmr.2015.3552
MLA
Liu, Y., Ban, L., Su, X., Gao, S., Liu, J., Cui, X."Effects of cinobufacini injection on cell proliferation and the expression of topoisomerases in human HepG‑2 hepatocellular carcinoma cells". Molecular Medicine Reports 12.1 (2015): 1598-1604.
Chicago
Liu, Y., Ban, L., Su, X., Gao, S., Liu, J., Cui, X."Effects of cinobufacini injection on cell proliferation and the expression of topoisomerases in human HepG‑2 hepatocellular carcinoma cells". Molecular Medicine Reports 12, no. 1 (2015): 1598-1604. https://doi.org/10.3892/mmr.2015.3552
Copy and paste a formatted citation
x
Spandidos Publications style
Liu Y, Ban LY, Su X, Gao S, Liu JW and Cui XN: Effects of cinobufacini injection on cell proliferation and the expression of topoisomerases in human HepG‑2 hepatocellular carcinoma cells. Mol Med Rep 12: 1598-1604, 2015.
APA
Liu, Y., Ban, L., Su, X., Gao, S., Liu, J., & Cui, X. (2015). Effects of cinobufacini injection on cell proliferation and the expression of topoisomerases in human HepG‑2 hepatocellular carcinoma cells. Molecular Medicine Reports, 12, 1598-1604. https://doi.org/10.3892/mmr.2015.3552
MLA
Liu, Y., Ban, L., Su, X., Gao, S., Liu, J., Cui, X."Effects of cinobufacini injection on cell proliferation and the expression of topoisomerases in human HepG‑2 hepatocellular carcinoma cells". Molecular Medicine Reports 12.1 (2015): 1598-1604.
Chicago
Liu, Y., Ban, L., Su, X., Gao, S., Liu, J., Cui, X."Effects of cinobufacini injection on cell proliferation and the expression of topoisomerases in human HepG‑2 hepatocellular carcinoma cells". Molecular Medicine Reports 12, no. 1 (2015): 1598-1604. https://doi.org/10.3892/mmr.2015.3552
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