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Article Open Access

Identification of therapeutic targets for breast cancer using biological informatics methods

  • Authors:
    • Xuejian Liu
    • Yongzhen Ma
    • Wenchuan Yang
    • Xia Wu
    • Lihua Jiang
    • Xiangli Chen
  • View Affiliations / Copyright

    Affiliations: Department of Oncology, The People's Hospital of Linyi Economic and Technological Development Zone, Linyi, Shandong 276023, P.R. China, Department of Histological Embryology, Shandong Medical College, Linyi, Shandong 276000, P.R. China
    Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 1789-1795
    |
    Published online on: March 27, 2015
       https://doi.org/10.3892/mmr.2015.3565
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Abstract

The present study aimed to investigate the modular mechanisms underlying breast cancer and identify potential targets for breast cancer treatment. The differentially expressed genes (DEGs) between breast cancer and normal cells were assessed using microarray data obtained from the Gene Expression Omnibus database. Gene ontology (GO) and pathway enrichment analyses were performed in order to investigate the functions of these DEGs. Subsequently, the protein‑protein interaction (PPI) network was constructed using the Cytoscape software. The identified subnetworks were further analyzed using the Molecular Complex Detection plugin. In total, 571 genes (241 upregulated and 330 downregulated genes) were found to be differentially expressed between breast cancer and normal cells. The GO terms significantly enriched by DEGs included cell adhesion, immune response and extracellular region, while the most significant pathways included focal adhesion and complement and coagulation cascade pathways. The PPI network was established with 273 nodes and 718 edges, while fibronectin 1 (FN1, degrees score, 39), interleukin 6 (IL6; degree score, 96) and c‑Fos protein (degree score, 32) were identified as the hub proteins in subnetwork 2. These dysregulated genes were found to be involved in the development of breast cancer. The FN1, IL6 and FOS genes may therefore be potential targets in the treatment of breast cancer.
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1 

Powles TJ: Breast cancer prevention. Oncologist. 7:60–64. 2002. View Article : Google Scholar : PubMed/NCBI

2 

Yager JD and Davidson NE: Estrogen carcinogenesis in breast cancer. N Engl J Med. 354:270–282. 2006. View Article : Google Scholar : PubMed/NCBI

3 

Nathanson KN, Wooster R and Weber BL: Breast cancer genetics: what we know and what we need. Nat Med. 7:552–556. 2001. View Article : Google Scholar : PubMed/NCBI

4 

Wemer RS, McCormick B, Petrec J, et al: Arm edema in conservatively managed breast cancer: obesity is a major predictive factor. Radiology. 180:177–184. 1991. View Article : Google Scholar

5 

Clapp RW, Jacobs MM and Loechler EL: Environmental and occupational causes of cancer: new evidence 2005–2007. Rev Environ Health. 23:1–37. 2008. View Article : Google Scholar : PubMed/NCBI

6 

Mohankumar K, Pajaniradje S, Sridharan S, et al: Mechanism of apoptotic induction in human breast cancer cell, MCF-7, by an analog of curcumin in comparison with curcumin - an in vitro and in silico approach. Chem Biol Interact. 210:51–63. 2014. View Article : Google Scholar

7 

World Health Organization: World cancer report 2008. Boyle P and Levin B: IARC; 2008

8 

Price JT, Tiganis T, Agarwal A, Djakiew D and Thompson EW: Epidermal growth factor promotes MDA-MB-231 breast cancer cell migration through a phosphatidylinositol 3′-kinase and phospholipase C-dependent mechanism. Cancer Res. 59:5475–5478. 1999.PubMed/NCBI

9 

Dang TT and Pearson GW: Abstract A38: Breast cancer intrinsic subtype specific interactions with the microenvironment dictate the mechanism of tumor invasion. Cancer Res. 73(3 Supplement): A38. 2013. View Article : Google Scholar

10 

Wu K, Weng Z, Tao Q, et al: Stage-specific expression of breast cancer-specific gene γ-synuclein. Cancer Epidemiol Biomarkers Prev. 12:920–925. 2003.PubMed/NCBI

11 

Shou J, Massarweh S, Osborne CK, et al: Mechanisms of tamoxifen resistance: increased estrogen receptor-HER2/neu cross-talk in ER/HER2-positive breast cancer. J Natl Cancer Inst. 96:926–935. 2004. View Article : Google Scholar : PubMed/NCBI

12 

Pietras RJ, Arboleda J, Reese DM, et al: HER-2 tyrosine kinase pathway targets estrogen receptor and promotes hormone-independent growth in human breast cancer cells. Oncogene. 10:2435–2446. 1995.PubMed/NCBI

13 

Kubo M, Nakamura M, Tasaki A, et al: Hedgehog signaling pathway is a new therapeutic target for patients with breast cancer. Cancer Res. 64:6071–6074. 2004. View Article : Google Scholar : PubMed/NCBI

14 

Planche A, Bacac M, Provero P, et al: Identification of prognostic molecular features in the reactive stroma of human breast and prostate cancer. PLoS One. 6:e186402011. View Article : Google Scholar : PubMed/NCBI

15 

Irizarry RA, Hobbs B, Collin F, et al: Exploration, normalization, and summaries of high density oligonucleotide array probe level data. Biostatistics. 4:249–264. 2003. View Article : Google Scholar : PubMed/NCBI

16 

Smyth GK: Linear models and empirical bayes methods for assessing differential expression in microarray experiments. Stat Appl Genet Mol Biol. 3:Article3. 2004.

17 

Ashburner M, Ball CA, Blake JA, et al: Gene ontology: tool for the unification of biology. Nat Genet. 25:25–29. 2000. View Article : Google Scholar : PubMed/NCBI

18 

Altermann E and Klaenhammer TR: PathwayVoyager: pathway mapping using the kyoto encyclopedia of genes and genomes (KEGG) database. BMC Genomics. 6:602005. View Article : Google Scholar : PubMed/NCBI

19 

Szklarczyk D, Franceschini A, Kuhn M, et al: The STR ING database in 2011: functional interaction networks of proteins, globally integrated and scored. Nucleic Acids Res. 39:D561–D568. 2011. View Article : Google Scholar

20 

Kohl M, Wiese S and Warscheid B: Cytoscape: software for visualization and analysis of biological networks. Methods Mol Biol. 696:291–303. 2011.

21 

Bader GD and Hogue CW: An automated method for finding molecular complexes in large protein interaction networks. BMC Bioinformatics. 4:22003. View Article : Google Scholar : PubMed/NCBI

22 

van ’t Veer LJ, Dai H, van de Vijver MJ, et al: Gene expression profiling predicts clinical outcome of breast cancer. Nature. 415:530–536. 2002. View Article : Google Scholar

23 

Wang Y, Klijn JG, Zhang Y, et al: Gene-expression profiles to predict distant metastasis of lymph-node-negative primary breast cancer. Lancet. 365:671–679. 2005. View Article : Google Scholar : PubMed/NCBI

24 

Gumbiner BM: Cell adhesion: The molecular basis of tissue architecture and morphogenesis. Cell. 84:345–357. 1996. View Article : Google Scholar : PubMed/NCBI

25 

Albelda SM and Buck CA: Integrins and other cell adhesion molecules. FASEB J. 4:2868–2880. 1990.PubMed/NCBI

26 

Albelda S: Role of integrins and other cell adhesion molecules in tumor progression and metastasis. Lab Invest. 68:4–17. 1993.PubMed/NCBI

27 

Siitonen SM, Kononen JT, Helin HJ, Rantala IS, Holli KA and Isola JJ: Reduced E-cadherin expression is associated with invasiveness and unfavorable prognosis in breast cancer. Am J Clin Pathol. 105:394–402. 1996.PubMed/NCBI

28 

Oka H, Shiozaki H, Kobayashi K, et al: Expression of E-cadherin cell adhesion molecules in human breast cancer tissues and its relationship to metastasis. Cancer Res. 53:1696–1701. 1993.PubMed/NCBI

29 

Saadatmand S, De Kruijf E, Sajet A, et al: Expression of cell adhesion molecules and prognosis in breast cancer. Br J Surg. 100:252–260. 2013. View Article : Google Scholar

30 

Springer TA: Adhesion receptors of the immune system. Nature. 346:425–434. 1990. View Article : Google Scholar : PubMed/NCBI

31 

Pankov R and Yamada KM: Fibronectin at a glance. J Cell Sci. 115:3861–3863. 2002. View Article : Google Scholar : PubMed/NCBI

32 

Steffens S, Schrader A, Vetter G, et al: Fibronectin 1 protein expression in clear cell renal cell carcinoma. Oncol Lett. 3:787–790. 2012.PubMed/NCBI

33 

Kondo N, Toyama T, Sugiura H, Fujii Y and Yamashita H: miR-206 expression is down-regulated in estrogen receptor alpha-positive human breast cancer. Cancer Res. 68:5004–5008. 2008. View Article : Google Scholar : PubMed/NCBI

34 

Adams BD, Claffey KP and White BA: Argonaute-2 expression is regulated by epidermal growth factor receptor and mitogen-activated protein kinase signaling and correlates with a transformed phenotype in breast cancer cells. Endocrinology. 150:14–23. 2009. View Article : Google Scholar :

35 

Mutlu P, Ural AU and Gunduz U: Differential gene expression analysis related to extracellular matrix components in drug-resistant RPMI-8226 cell line. Biomed Pharmacother. 66:228–231. 2012. View Article : Google Scholar : PubMed/NCBI

36 

Milde-Langosch K: The Fos family of transcription factors and their role in tumourigenesis. Eur J Cancer. 41:2449–2461. 2005. View Article : Google Scholar : PubMed/NCBI

37 

Lee AV, Cui X and Oesterreich S: Cross-talk among estrogen receptor, epidermal growth factor, and insulin-like growth factor signaling in breast cancer. Clin Cancer Res. 7:4429s–4435s. 2001.

38 

Hanstein B, Djahansouzi S, Dall P, Beckmann M and Bender H: Insights into the molecular biology of the estrogen receptor define novel therapeutic targets for breast cancer. Eur J Endocrinol. 150:243–255. 2004. View Article : Google Scholar : PubMed/NCBI

39 

Morishita S, Niwa K, Ichigo S, et al: Overexpressions of c-fos/jun mRNA and their oncoproteins (Fos/Jun) in the mouse uterus treated with three natural estrogens. Cancer Lett. 97:225–231. 1995. View Article : Google Scholar : PubMed/NCBI

40 

Musgrove EA, Lee C and Sutherland RL: Progestins both stimulate and inhibit breast cancer cell cycle progression while increasing expression of transforming growth factor alpha, epidermal growth factor receptor, c-fos, and c-myc genes. Mol Cell Biol. 11:5032–5043. 1991.PubMed/NCBI

41 

Sutherland RL, Prall OW, Watts CK and Musgrove EA: Estrogen and progestin regulation of cell cycle progression. J Mammary Gland Biol Neoplasia. 3:63–72. 1998. View Article : Google Scholar

42 

Xing Z, Gauldie J, Cox G, Baumann H, Jordana M, Lei XF and Achong MK: IL-6 is an antiinflammatory cytokine required for controlling local or systemic acute infammatory responses. J Clin Invest. 101:311–320. 1998. View Article : Google Scholar : PubMed/NCBI

43 

He G, Dhar D, Nakagawa H, et al: Identification of liver cancer progenitors whose malignant progression depends on autocrine IL-6 signaling. Cell. 155:384–396. 2013. View Article : Google Scholar : PubMed/NCBI

44 

Seifart C, Plagens A, Dempfle A, et al: TNF-α, TNF-β, IL-6, and IL-10 polymorphisms in patients with lung cancer. Dis Markers. 21:157–165. 2013. View Article : Google Scholar

45 

Hartman ZC, Poage GM, Den Hollander P, et al: Growth of triple-negative breast cancer cells relies upon coordinate autocrine expression of the proinflammatory cytokines IL-6 and IL-8. Cancer Res. 73:3470–3480. 2013. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Liu X, Ma Y, Yang W, Wu X, Jiang L and Chen X: Identification of therapeutic targets for breast cancer using biological informatics methods. Mol Med Rep 12: 1789-1795, 2015.
APA
Liu, X., Ma, Y., Yang, W., Wu, X., Jiang, L., & Chen, X. (2015). Identification of therapeutic targets for breast cancer using biological informatics methods. Molecular Medicine Reports, 12, 1789-1795. https://doi.org/10.3892/mmr.2015.3565
MLA
Liu, X., Ma, Y., Yang, W., Wu, X., Jiang, L., Chen, X."Identification of therapeutic targets for breast cancer using biological informatics methods". Molecular Medicine Reports 12.2 (2015): 1789-1795.
Chicago
Liu, X., Ma, Y., Yang, W., Wu, X., Jiang, L., Chen, X."Identification of therapeutic targets for breast cancer using biological informatics methods". Molecular Medicine Reports 12, no. 2 (2015): 1789-1795. https://doi.org/10.3892/mmr.2015.3565
Copy and paste a formatted citation
x
Spandidos Publications style
Liu X, Ma Y, Yang W, Wu X, Jiang L and Chen X: Identification of therapeutic targets for breast cancer using biological informatics methods. Mol Med Rep 12: 1789-1795, 2015.
APA
Liu, X., Ma, Y., Yang, W., Wu, X., Jiang, L., & Chen, X. (2015). Identification of therapeutic targets for breast cancer using biological informatics methods. Molecular Medicine Reports, 12, 1789-1795. https://doi.org/10.3892/mmr.2015.3565
MLA
Liu, X., Ma, Y., Yang, W., Wu, X., Jiang, L., Chen, X."Identification of therapeutic targets for breast cancer using biological informatics methods". Molecular Medicine Reports 12.2 (2015): 1789-1795.
Chicago
Liu, X., Ma, Y., Yang, W., Wu, X., Jiang, L., Chen, X."Identification of therapeutic targets for breast cancer using biological informatics methods". Molecular Medicine Reports 12, no. 2 (2015): 1789-1795. https://doi.org/10.3892/mmr.2015.3565
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