Knockdown of reticulon 4C by lentivirus inhibits human colorectal cancer cell growth

Xue,Hui; Wang,Zheng; Chen,Jinxian; Yang,Zeyu; Tang,Jian

doi:10.3892/mmr.2015.3569

August-2015 Volume 12 Issue 2

Full Size Image

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

An International Open Access Journal Devoted to General Medicine.

August-2015 Volume 12 Issue 2

Full Size Image

Article

Knockdown of reticulon 4C by lentivirus inhibits human colorectal cancer cell growth

Authors:
- Hui Xue
- Zheng Wang
- Jinxian Chen
- Zeyu Yang
- Jian Tang
View Affiliations / Copyright

Affiliations: Neijing Institution of the Basic Medical College, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, P.R. China, Department of General Surgery, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, P.R. China
Pages: 2063-2067
|
Published online on: March 31, 2015

https://doi.org/10.3892/mmr.2015.3569
Expand metrics +

Abstract

Colorectal cancer is the third most common type of cancer worldwide with high cell motility and metastatic potential. Reticulon 4C (RTN4-C) is the shortest isoform of the reticulon family protein RTN4, which may act to induce cell apoptosis and suppress tumor development. The aim of the present study was to determine the role of RTN4‑C in colorectal cancer, and potentially identify a novel target for anti‑tumor therapy. To investigate the biological role of RTN4‑C in colorectal cancer, the expression levels of RTN4‑C were initially analyzed in six colorectal cancer cell lines by reverse transcription‑quantitative polymerase chain reaction and western blot analysis. In addition, lentivirus‑based RNA interference was utilized to knock down RTN4‑C expression in RKO and DLD‑1 cells with low and high levels of RTN4‑C, respectively. The rate of proliferation decreased in RTN4‑C silenced RKO and DLD‑1 cells compared with the control, as determined using MTT and colony formation assays. Flow cytometric analysis revealed that RTN4‑C knockdown in RKO cells led to cell cycle arrest at the G0/G1 phase, particularly at the sub‑G1 phase representing apoptotic cells. These results indicate that RTN4‑C has an important role in colorectal cancer cell growth, which may provide a potential therapeutic approach for human colorectal cancer.

View Figures

View References

1	Nasrallah A, Saykali B, Al Dimassi S, Khoury N, Hanna S and El-Sibai M: Effect of StarD13 on colorectal cancer proliferation, motility and invasion. Oncol Rep. 31:505–515. 2014.
2	Mathis KL and Nelson H: Controversies in laparoscopy for colon and rectal cancer. Surg Oncol Clin N Am. 23:35–47. 2014. View Article : Google Scholar
3	Watson AJ and Collins PD: Colon cancer: a civilization disorder. Dig Dis. 29:222–228. 2011. View Article : Google Scholar : PubMed/NCBI
4	Chambers AF, Groom AC and MacDonald IC: Metastasis: dissemination and growth of cancer cells in metastatic sites. Nat Rev Cancer. 2:563–572. 2002. View Article : Google Scholar : PubMed/NCBI
5	Shakibaei M, Buhrmann C, Kraehe P, Shayan P, Lueders C and Goel A: Curcumin chemosensitizes 5-fluorouracil resistant mmr-deficient human colon cancer cells in high density cultures. PLoS One. 9:e853972014. View Article : Google Scholar : PubMed/NCBI
6	Chiurchiu V, Maccarrone M and Orlacchio A: The role of reticulons in neurodegenerative diseases. Neuromolecular Med. 16:3–15. 2014. View Article : Google Scholar :
7	Diekmann H, Klinger M, Oertle T, et al: Analysis of the reticulon gene family demonstrates the absence of the neurite growth inhibitor Nogo-A in fish. Mol Biol Evol. 22:1635–1648. 2005. View Article : Google Scholar : PubMed/NCBI
8	Zhu L, Xiang R, Dong W, Liu Y and Qi Y: Anti-apoptotic activity of Bcl-2 is enhanced by its interaction with RTN3. Cell Biol Int. 31:825–830. 2007. View Article : Google Scholar : PubMed/NCBI
9	Chen Y, Tang X, Cao X, Chen H and Zhang X: Human Nogo-C overexpression induces HEK293 cell apoptosis via a mechanism that involves JNK-c-Jun pathway. Biochem Biophys Res Commun. 348:923–928. 2006. View Article : Google Scholar : PubMed/NCBI
10	Chen YC, Lu DD, Cao XR and Zhang XR: RTN4-C gene expression in hepatocellular carcinoma and its influence on SMMC7721 cell growth and apoptosis. Yi Chuan Xue Bao. 32:891–897. 2005.PubMed/NCBI
11	Murray GI, Duncan ME, O’Neil P, Melvin WT and Fothergill JE: Matrix metalloproteinase-1 is associated with poor prognosis in colorectal cancer. Nat Med. 2:461–462. 1996. View Article : Google Scholar : PubMed/NCBI
12	Saltz LB, Cox JV, Blanke C, et al: Irinotecan plus fluorouracil and leucovorin for metastatic colorectal cancer Irinotecan Study Group. N Engl J Med. 343:905–914. 2000. View Article : Google Scholar : PubMed/NCBI
13	Halama N, Michel S, Kloor M, et al: Localization and density of immune cells in the invasive margin of human colorectal cancer liver metastases are prognostic for response to chemotherapy. Cancer Res. 71:5670–5677. 2011. View Article : Google Scholar : PubMed/NCBI
14	Liang L, Wong CM, Ying Q, et al: MicroRNA. 125b suppressesed human liver cancer cell proliferation and metastasis by directly targeting oncogene LIN28B2. Hepatology. 52:1731–1740. 2010. View Article : Google Scholar : PubMed/NCBI
15	Zhang B, Li KY, Chen HY, et al: Lentivirus-based RNA silencing of nemo-like kinase (NLK) inhibits the CAL 27 human adenosquamos carcinoma cells proliferation and blocks G0/G1 phase to S phase. Int J Med Sci. 10:1301–1306. 2013. View Article : Google Scholar : PubMed/NCBI
16	Zhao H, Ho PC, Lo YH, et al: Interaction of proliferation cell nuclear antigen (PCNA) with c-Abl in cell proliferation and response to DNA damages in breast cancer. PloS One. 7:e294162012. View Article : Google Scholar : PubMed/NCBI

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Knockdown of reticulon 4C by lentivirus inhibits human colorectal cancer cell growth

This article is mentioned in:

Abstract

Figure 1

Figure 2

Figure 3

Figure 4

Related Articles