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Sann-Joong-Kuey-Jian-Tang induces autophagy in HepG2 cells via regulation of the phosphoinositide-3 kinase/Akt/mammalian target of rapamycin and p38 mitogen-activated protein kinase pathways

  • Authors:
    • Wan-Ling Chuang
    • Chin-Cheng Su
    • Ping-Yi Lin
    • Chi-Chen Lin
    • Yao-Li Chen
  • View Affiliations / Copyright

    Affiliations: Transplant Medicine and Surgery Research Centre, Changhua Christian Hospital, Changhua 50006, Taiwan, R.O.C., Department of Surgery, Changhua Christian Hospital, Changhua 50006, Taiwan, R.O.C., Institute of Biomedical Science, National Chung-Hsing University, Taichung 40227, Taiwan, R.O.C.
    Copyright: © Chuang et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 1677-1684
    |
    Published online on: March 31, 2015
       https://doi.org/10.3892/mmr.2015.3573
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Abstract

Sann-Joong-Kuey-Jian‑Tang (SJKJT), a traditional Chinese medicine, was previously reported to induce autophagy and inhibit the proliferation of the human HepG2 hepatocellular carcinoma cell line via an extrinsic pathway. In the present study, the effects of SJKJT‑induced autophagy and the cytotoxic mechanisms mediating these effects were investigated in HepG2 cells. The cytotoxicity of SJKJT in the HepG2 cells was evaluated using a 3‑(4,5‑dimethylthiazol‑2‑yl)‑2,5‑diphenyltetrazolium bromide assay. The results demonstrated that the half‑maximal inhibitory concentration of SJKJT was 2.91 mg/ml at 24 h, 1.64 mg/ml at 48 h and 1.26 mg/ml at 72 h. The results of confocal fluorescence microscopy indicated that SJKJT resulted in the accumulation of green fluorescent protein‑LC3 and vacuolation of the cytoplasm. Flow cytometric analysis revealed the accumulation of acidic vesicular organelles. Furthermore, western blot analysis, used to determine the expression levels of autophagy‑associated proteins, demonstrated that the HepG2 cells treated with SJKJT exhibited LC3B‑Ⅰ/LC3B‑Ⅱ conversion, increased expression levels of Beclin, Atg‑3 and Atg‑5 and reduced expression levels of p62 and decreased signaling of the phosphoinositide‑3 kinase/Akt/mammalian target of rapamycin and the p38 mitogen‑activated protein kinase pathways. Taken together, these findings may assist in the development of novel chemotherapeutic agents for the treatment of malignant types of liver cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Chuang W, Su C, Lin P, Lin C and Chen Y: Sann-Joong-Kuey-Jian-Tang induces autophagy in HepG2 cells via regulation of the phosphoinositide-3 kinase/Akt/mammalian target of rapamycin and p38 mitogen-activated protein kinase pathways. Mol Med Rep 12: 1677-1684, 2015.
APA
Chuang, W., Su, C., Lin, P., Lin, C., & Chen, Y. (2015). Sann-Joong-Kuey-Jian-Tang induces autophagy in HepG2 cells via regulation of the phosphoinositide-3 kinase/Akt/mammalian target of rapamycin and p38 mitogen-activated protein kinase pathways. Molecular Medicine Reports, 12, 1677-1684. https://doi.org/10.3892/mmr.2015.3573
MLA
Chuang, W., Su, C., Lin, P., Lin, C., Chen, Y."Sann-Joong-Kuey-Jian-Tang induces autophagy in HepG2 cells via regulation of the phosphoinositide-3 kinase/Akt/mammalian target of rapamycin and p38 mitogen-activated protein kinase pathways". Molecular Medicine Reports 12.2 (2015): 1677-1684.
Chicago
Chuang, W., Su, C., Lin, P., Lin, C., Chen, Y."Sann-Joong-Kuey-Jian-Tang induces autophagy in HepG2 cells via regulation of the phosphoinositide-3 kinase/Akt/mammalian target of rapamycin and p38 mitogen-activated protein kinase pathways". Molecular Medicine Reports 12, no. 2 (2015): 1677-1684. https://doi.org/10.3892/mmr.2015.3573
Copy and paste a formatted citation
x
Spandidos Publications style
Chuang W, Su C, Lin P, Lin C and Chen Y: Sann-Joong-Kuey-Jian-Tang induces autophagy in HepG2 cells via regulation of the phosphoinositide-3 kinase/Akt/mammalian target of rapamycin and p38 mitogen-activated protein kinase pathways. Mol Med Rep 12: 1677-1684, 2015.
APA
Chuang, W., Su, C., Lin, P., Lin, C., & Chen, Y. (2015). Sann-Joong-Kuey-Jian-Tang induces autophagy in HepG2 cells via regulation of the phosphoinositide-3 kinase/Akt/mammalian target of rapamycin and p38 mitogen-activated protein kinase pathways. Molecular Medicine Reports, 12, 1677-1684. https://doi.org/10.3892/mmr.2015.3573
MLA
Chuang, W., Su, C., Lin, P., Lin, C., Chen, Y."Sann-Joong-Kuey-Jian-Tang induces autophagy in HepG2 cells via regulation of the phosphoinositide-3 kinase/Akt/mammalian target of rapamycin and p38 mitogen-activated protein kinase pathways". Molecular Medicine Reports 12.2 (2015): 1677-1684.
Chicago
Chuang, W., Su, C., Lin, P., Lin, C., Chen, Y."Sann-Joong-Kuey-Jian-Tang induces autophagy in HepG2 cells via regulation of the phosphoinositide-3 kinase/Akt/mammalian target of rapamycin and p38 mitogen-activated protein kinase pathways". Molecular Medicine Reports 12, no. 2 (2015): 1677-1684. https://doi.org/10.3892/mmr.2015.3573
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