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Article

Particulate matter 2.5 induces autophagy via inhibition of the phosphatidylinositol 3-kinase/Akt/mammalian target of rapamycin kinase signaling pathway in human bronchial epithelial cells

  • Authors:
    • Tie Liu
    • Bin Wu
    • Yahong Wang
    • Huijuan He
    • Ziying Lin
    • Jianxin Tan
    • Lawei Yang
    • David W. Kamp
    • Xu Zhou
    • Jinfeng Tang
    • Haili Huang
    • Liangqing Zhang
    • Liu Bin
    • Gang Liu
  • View Affiliations / Copyright

    Affiliations: Clinical Research Center, Guangdong Medical College, Zhanjiang, Guangdong 524001, P.R. China, Department of Respiratory Medicine, Affiliated Hospital of Guangdong Medical College, Zhanjiang, Guangdong 524001, P.R. China, Department of Medicine, Division of Pulmonary and Critical Care Medicine, Northwestern University Feinberg School of Medicine and Jesse Brown VA Medical Center, Chicago, IL 60611, USA, Department of Immunology and Tumor Research Institute, The First Affiliated Hospital, Medical School of Xi'an Jiaotong University, Xi'an, Shanxi 710061, P.R. China
  • Pages: 1914-1922
    |
    Published online on: March 31, 2015
       https://doi.org/10.3892/mmr.2015.3577
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Abstract

Particulate matter 2.5 (PM2.5) is a significant risk factor for asthma. A recent study revealed that autophagy was associated with asthma pathogenesis. However, the specific mechanisms underlying PM2.5‑induced autophagy in asthma have remained elusive. In the present study, PM2.5‑induced autophagy was evaluated in Beas‑2B human bronchial epithelial cells and the potential molecular mechanisms were investigated. Using electron microscopy, immunofluorescence staining and immunoblot studies, it was confirmed that PM2.5 induced autophagy in Beas‑2B cells as a result of PM2.5‑mediated inhibition of the phosphatidylinositol 3‑kinase (PI3K)/Akt/mammalian target of rapamycin (mTOR) pathway in Beas‑2B cells. LY294002, a PI3K inhibitor, reduced the accumulation of microtubule‑associated protein 1 light chain 3 II and attenuated the effect of PM2.5. Phosphorylated (p‑)p38, p‑extracellular signal‑regulated kinase and p‑c‑Jun N‑terminal kinase were dephosphorylated following exposure to PM2.5. The roles of p53, reactive oxygen species scavenger tetramethylthiourea and autophagy inhibitor 3‑methyladenine in PM2.5‑induced autophagy in Beas‑2B cells were also investigated. The results suggested that the PI3K/Akt/mTOR signaling pathway may be a key contributor to PM2.5‑induced autophagy in Beas‑2B cells. The results of the present study therefore provided an a insight into potential future clinical applications targeting these signaling pathways, for the prevention and/or treatment of PM2.5‑induced lung diseases.
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Copy and paste a formatted citation
Spandidos Publications style
Liu T, Wu B, Wang Y, He H, Lin Z, Tan J, Yang L, Kamp DW, Zhou X, Tang J, Tang J, et al: Particulate matter 2.5 induces autophagy via inhibition of the phosphatidylinositol 3-kinase/Akt/mammalian target of rapamycin kinase signaling pathway in human bronchial epithelial cells. Mol Med Rep 12: 1914-1922, 2015.
APA
Liu, T., Wu, B., Wang, Y., He, H., Lin, Z., Tan, J. ... Liu, G. (2015). Particulate matter 2.5 induces autophagy via inhibition of the phosphatidylinositol 3-kinase/Akt/mammalian target of rapamycin kinase signaling pathway in human bronchial epithelial cells. Molecular Medicine Reports, 12, 1914-1922. https://doi.org/10.3892/mmr.2015.3577
MLA
Liu, T., Wu, B., Wang, Y., He, H., Lin, Z., Tan, J., Yang, L., Kamp, D. W., Zhou, X., Tang, J., Huang, H., Zhang, L., Bin, L., Liu, G."Particulate matter 2.5 induces autophagy via inhibition of the phosphatidylinositol 3-kinase/Akt/mammalian target of rapamycin kinase signaling pathway in human bronchial epithelial cells". Molecular Medicine Reports 12.2 (2015): 1914-1922.
Chicago
Liu, T., Wu, B., Wang, Y., He, H., Lin, Z., Tan, J., Yang, L., Kamp, D. W., Zhou, X., Tang, J., Huang, H., Zhang, L., Bin, L., Liu, G."Particulate matter 2.5 induces autophagy via inhibition of the phosphatidylinositol 3-kinase/Akt/mammalian target of rapamycin kinase signaling pathway in human bronchial epithelial cells". Molecular Medicine Reports 12, no. 2 (2015): 1914-1922. https://doi.org/10.3892/mmr.2015.3577
Copy and paste a formatted citation
x
Spandidos Publications style
Liu T, Wu B, Wang Y, He H, Lin Z, Tan J, Yang L, Kamp DW, Zhou X, Tang J, Tang J, et al: Particulate matter 2.5 induces autophagy via inhibition of the phosphatidylinositol 3-kinase/Akt/mammalian target of rapamycin kinase signaling pathway in human bronchial epithelial cells. Mol Med Rep 12: 1914-1922, 2015.
APA
Liu, T., Wu, B., Wang, Y., He, H., Lin, Z., Tan, J. ... Liu, G. (2015). Particulate matter 2.5 induces autophagy via inhibition of the phosphatidylinositol 3-kinase/Akt/mammalian target of rapamycin kinase signaling pathway in human bronchial epithelial cells. Molecular Medicine Reports, 12, 1914-1922. https://doi.org/10.3892/mmr.2015.3577
MLA
Liu, T., Wu, B., Wang, Y., He, H., Lin, Z., Tan, J., Yang, L., Kamp, D. W., Zhou, X., Tang, J., Huang, H., Zhang, L., Bin, L., Liu, G."Particulate matter 2.5 induces autophagy via inhibition of the phosphatidylinositol 3-kinase/Akt/mammalian target of rapamycin kinase signaling pathway in human bronchial epithelial cells". Molecular Medicine Reports 12.2 (2015): 1914-1922.
Chicago
Liu, T., Wu, B., Wang, Y., He, H., Lin, Z., Tan, J., Yang, L., Kamp, D. W., Zhou, X., Tang, J., Huang, H., Zhang, L., Bin, L., Liu, G."Particulate matter 2.5 induces autophagy via inhibition of the phosphatidylinositol 3-kinase/Akt/mammalian target of rapamycin kinase signaling pathway in human bronchial epithelial cells". Molecular Medicine Reports 12, no. 2 (2015): 1914-1922. https://doi.org/10.3892/mmr.2015.3577
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