Upregulation of neuroglobin expression and changes in serum redox indices in a rat model of middle cerebral artery occlusion

Shang,Aijia; Yang,Ying; Wang,Hangyan; Wang,Jing; Hang,Xingyi; Wang,Zhongfeng; Zhou,Dingbiao

doi:10.3892/mmr.2015.3593

August-2015 Volume 12 Issue 2

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August-2015 Volume 12 Issue 2

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Article Open Access

Upregulation of neuroglobin expression and changes in serum redox indices in a rat model of middle cerebral artery occlusion

Authors:
- Aijia Shang
- Ying Yang
- Hangyan Wang
- Jing Wang
- Xingyi Hang
- Zhongfeng Wang
- Dingbiao Zhou
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Affiliations: Department of Neurosurgery, General Hospital of Chinese People's Liberation Army, Beijing 100853, P.R. China, Department of Health Medicine, General Hospital of Chinese People's Liberation Army, Beijing 100853, P.R. China, Department of Pediatrics, General Hospital of Chinese People's Liberation Army, Beijing 100853, P.R. China, National Center for Scientific Data Sharing for Population and Health, Beijing 100730, P.R. China, Medical Neurobiology of State Key Laboratory, Fudan University, Shanghai 200032, P.R. China

Copyright: © Shang et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
Pages: 1693-1698
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Published online on: April 3, 2015

https://doi.org/10.3892/mmr.2015.3593
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Abstract

Neuroglobin (NGB) is a recently identified protein, which is localized in the neurons and retinal cells of the central and peripheral nervous systems in vertebrates. It is hypothesized to function as a scavenger for reactive oxygen species, or as a stress‑responsive sensor for signal transduction in hypoxic‑ischemic brain insults. However, the mechanism underlying the physiological function of this protein remains to be elucidated. In the present study, the profiling of changes in the serum redox index of morphological features of the hippocampus and cortex, and of the expression of NGB and hypoxia‑inducible factor‑1α (HIF‑1α), are described in a rat middle cerebral artery occlusion (MCAO) model. The necrotic zone of the rat neural tissues increased in size with increasing reperfusion time, and different brain slices exhibited necrosis in different regions. The number of NGB‑positive hippocampal and cortical cells, as well as NGB and HIF‑1α transcript and protein levels in the ischemic cortex, increased with increasing reperfusion time. NGB and HIF‑1α mRNA and protein levels peaked in the group that received reperfusion at 32 h after MCAO. These findings indicated that HIF‑1α may be involved in ischemic pathology in an MCAO model and that NGB expression may be upregulated. Serum superoxide dismutase (SOD) activity decreased and serum malondialdehyde (MDA) levels increased with increasing reperfusion time, indicating that the redox potential increased following MCAO. Serum SOD and MDA measurements may, therefore, be useful as biomarkers for the early detection of ischemic injury in a clinical setting.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Upregulation of neuroglobin expression and changes in serum redox indices in a rat model of middle cerebral artery occlusion

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