Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
August-2015 Volume 12 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
August-2015 Volume 12 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Assessment of the embryotoxicity of four Chinese herbal extracts using the embryonic stem cell test

  • Authors:
    • Lin‑Yan Li
    • Fen‑Fang Cao
    • Zhi‑Jian Su
    • Qi‑Hao Zhang
    • Xiao‑Yong Dai
    • Xue Xiao
    • Ya‑Dong Huang
    • Qing Zheng
    • Hua Xu
  • View Affiliations / Copyright

    Affiliations: Department of Microbiological and Biochemical Pharmacy, College of Pharmacy, Jinan University, Guangzhou, Guangdong 510632, P.R. China, Department of Biopharmaceutical Research and Development Centre, Jinan University, Guangzhou, Guangdong 510632, P.R. China
  • Pages: 2348-2354
    |
    Published online on: April 7, 2015
       https://doi.org/10.3892/mmr.2015.3598
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Rhizoma Atractylodes macrocephala, Radix Isatidis, Coptis chinensis and Flos Genkwa are common herbal remedies used by pregnant woman in China. In this study, their potential embryotoxicity was assessed using the embryonic stem cell test (EST) and a prediction model. The potential embryotoxicity of the herbs was based on three endpoints: the concentrations of the compounds that inhibited the proliferation of 50% of embryonic stem cells (ESCs) (IC50ES), the concentrations that inhibited 50% of 3T3 cells (IC503T3), and the concentrations that inhibited the differentiation of 50% of ESCs (ID50ES). The results revealed that Rhizoma Atractylodes macrocephala and Radix Isatidis are non‑embryotoxic compounds. Coptis chinensis extracts appeared to demonstrated weak embryotoxicity, and Flos Genkwa exhibited strong embryotoxicity. These results may be useful in guiding the clinical use of these herbs and in expanding the application of the EST to the field of traditional Chinese medicine.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Figure 7

View References

1 

Lu F, Cao M, Wu B, et al: Urinary metabonomics study on toxicity biomarker discovery in rats treated with Xanthii Fructus. J Ethnopharmacol. 149:311–320. 2013. View Article : Google Scholar : PubMed/NCBI

2 

Lu F, Gu Q, Wu R and Xu J: A structure-similarity-based software for the cardiovascular toxicity prediction of traditional Chinese medicine. Bioinformation. 8:110–113. 2012. View Article : Google Scholar : PubMed/NCBI

3 

Zhang ZH, Zhao YY, Cheng XL, et al: General toxicity of Pinellia ternata (Thunb) Berit in rat: a metabonomic method for profiling of serum metabolic changes. J Ethnopharmacol. 149:303–310. 2013. View Article : Google Scholar : PubMed/NCBI

4 

Chuang CH, Hsieh WS, Guo YL, et al: Chinese herbal medicines used in pregnancy: a population-based survey in Taiwan. Pharmacoepidemiol Drug Saf. 16:464–468. 2007. View Article : Google Scholar

5 

Nordeng H and Havnen GC: Use of herbal drugs in pregnancy: a survey among 400 Norwegian women. Pharmacoepidemiol Drug Saf. 13:371–380. 2004. View Article : Google Scholar : PubMed/NCBI

6 

Xu CL, Zhao YF, Shang XY and Niu WN: The effects of supplementing diets with Atractylodes macrocephala Koidz rhizomes on growth performance and immune function in piglets. J Animal Feed Sci. 21:302–312. 2012.

7 

Jin C, Zhang PJ, Bao CQ, et al: Protective effects of Atractylodes macrocephala polysaccharide on liver ischemia-reperfusion injury and its possible mechanism in rats. Am J Chin Med. 39:489–502. 2011. View Article : Google Scholar : PubMed/NCBI

8 

Zheng HZ, Dong ZH and Yu J: Modern Study of Traditional. Chin Med J. 328–334. 1997.

9 

Xiao PG and Lian WY: The Illustrated Medicinal Plants of China. Shanghai Education Press; Shanghai: 1998

10 

Zhou BN: Some progress on the chemistry of natural bioactive terpenoids from Chinese medicinal plants. Mem Inst Oswaldo Cruz. 86(Suppl 2): 219–226. 1991. View Article : Google Scholar : PubMed/NCBI

11 

Lee MY, Park BY, Kwon OK, et al: Anti-inflammatory activity of (−)-aptosimon isolated from Daphne genkwa in RAW264.7 cells. Int Immunopharmacol. 9:878–885. 2009. View Article : Google Scholar : PubMed/NCBI

12 

Zhao Y, Yuan S, Li A, Zhang B and Wang Z: Effects of processing on toxicity and pharmacological action of Flos Genkwa. Zhongguo Zhong Yao Za Zhi. 23:344–347. 382–343. 1998.In Chinese.

13 

Hong JY, Chung HJ, Lee HJ, Park HJ and Lee SK: Growth inhibition of human lung cancer cells via down-regulation of epidermal growth factor receptor signaling by yuanhuadine, a daphnane diterpene from Daphne genkwa. J Nat Prod. 74:2102–2108. 2011. View Article : Google Scholar : PubMed/NCBI

14 

Goel RK, Prabha T, Kumar MM, Dorababu M, Prakash and Singh G: Teratogenicity of Asparagus racemosus Willd root, a herbal medicine. Indian J Exp Biol. 44:570–573. 2006.PubMed/NCBI

15 

Moallem SA, Ahmadi A, Moshafi M and Taghavi MM: Teratogenic effects of HESA-A, a natural anticancer product from Iran, in mice. Hum Exp Toxicol. 30:851–859. 2011. View Article : Google Scholar

16 

Chen CC and Chan WH: Impact effects of puerarin on mouse embryonic development. Reprod Toxicol. 28:530–535. 2009. View Article : Google Scholar : PubMed/NCBI

17 

de Jong E, Barenys M, Hermsen SA, et al: Comparison of the mouse Embryonic Stem cell Test, the rat Whole Embryo Culture and the Zebrafish Embryotoxicity Test as alternative methods for developmental toxicity testing of six 1,2,4-triazoles. Toxicol Appl Pharmacol. 253:103–111. 2011. View Article : Google Scholar : PubMed/NCBI

18 

Spielmann H and Liebsch M: Lessons learned from validation of in vitro toxicity test: from failure to acceptance into regulatory practice. Toxicol In Vitro. 15:585–590. 2001. View Article : Google Scholar : PubMed/NCBI

19 

Bremer S, Worth AP, Paparella M, et al: Establishment of an in vitro reporter gene assay for developmental cardiac toxicity. Toxicology In Vitro. 15:215–223. 2001. View Article : Google Scholar : PubMed/NCBI

20 

Genschow E, Scholz G, Brown N, et al: Development of prediction models for three in vitro embryotoxicity tests in an ECVAM validation study. In Vitr Mol Toxicol. 13:51–66. 2000.PubMed/NCBI

21 

Whitlow S, Bürgin H and Clemann N: The embryonic stem cell test for the early selection of pharmaceutical compounds. ALTEX. 24:3–7. 2007.PubMed/NCBI

22 

Paquette JA, Kumpf SW, Streck RD, Thomson JJ, Chapin RE and Stedman DB: Assessment of the Embryonic Stem Cell Test and application and use in the pharmaceutical industry. Birth Defects Res B Dev Reprod Toxicol. 83:104–111. 2008. View Article : Google Scholar : PubMed/NCBI

23 

Kong D, Xing L, Liu R, et al: Individual and combined developmental toxicity assessment of bisphenol A and genistein using the embryonic stem cell test in vitro. Food Chem Toxicol. 60:497–505. 2013. View Article : Google Scholar : PubMed/NCBI

24 

Zhang W, Song DR, Wang YN and Zhu Z: Evaluation of embryotoxicity of baicalin based on embryonic stem cell test system. Chin J Pharmacol Toxicol. 26:864–869. 2012.

25 

Spielmann H and Liebsch M: Validation successes: chemicals. Altern Lab Anim. 30(Suppl 2): 33–40. 2002.

26 

Zhao AS, Sun YL and Zhang LS: Safety assessment of Baizhu. Chin J Public Health. 1:43–45. 2006.

27 

Wang HY, Ma YF and Zhou WY: On IRPS toxicity test and effects on immune system. Journal of Mianyang Normal University. 31:52012.

28 

Pang Z, Tang J, Zhu W and Lu Y: Genetic toxicity of water boiled juice of Isatic Tinctoria L. in mice. Academic Journal of Guangzhou Medical College. 28:41–42. 2000.

29 

Qiu SH, Tang WB, Li FY, Xiao JR and Chen BY: Experimental study of the acute toxicity on common-used bitter and cold medicines. Central South Pharmacy. 2:37–38. 2004.

30 

Pasqual M, Lauer C, Moyna P and Henriques JA: Genotoxicity of isoquinoline alkaloid berberine in prokaryotic and eukaryotic organisms. Mutat Res. 286:243–152. 1993. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Li LY, Cao FF, Su ZJ, Zhang QH, Dai XY, Xiao X, Huang YD, Zheng Q and Xu H: Assessment of the embryotoxicity of four Chinese herbal extracts using the embryonic stem cell test. Mol Med Rep 12: 2348-2354, 2015.
APA
Li, L., Cao, F., Su, Z., Zhang, Q., Dai, X., Xiao, X. ... Xu, H. (2015). Assessment of the embryotoxicity of four Chinese herbal extracts using the embryonic stem cell test. Molecular Medicine Reports, 12, 2348-2354. https://doi.org/10.3892/mmr.2015.3598
MLA
Li, L., Cao, F., Su, Z., Zhang, Q., Dai, X., Xiao, X., Huang, Y., Zheng, Q., Xu, H."Assessment of the embryotoxicity of four Chinese herbal extracts using the embryonic stem cell test". Molecular Medicine Reports 12.2 (2015): 2348-2354.
Chicago
Li, L., Cao, F., Su, Z., Zhang, Q., Dai, X., Xiao, X., Huang, Y., Zheng, Q., Xu, H."Assessment of the embryotoxicity of four Chinese herbal extracts using the embryonic stem cell test". Molecular Medicine Reports 12, no. 2 (2015): 2348-2354. https://doi.org/10.3892/mmr.2015.3598
Copy and paste a formatted citation
x
Spandidos Publications style
Li LY, Cao FF, Su ZJ, Zhang QH, Dai XY, Xiao X, Huang YD, Zheng Q and Xu H: Assessment of the embryotoxicity of four Chinese herbal extracts using the embryonic stem cell test. Mol Med Rep 12: 2348-2354, 2015.
APA
Li, L., Cao, F., Su, Z., Zhang, Q., Dai, X., Xiao, X. ... Xu, H. (2015). Assessment of the embryotoxicity of four Chinese herbal extracts using the embryonic stem cell test. Molecular Medicine Reports, 12, 2348-2354. https://doi.org/10.3892/mmr.2015.3598
MLA
Li, L., Cao, F., Su, Z., Zhang, Q., Dai, X., Xiao, X., Huang, Y., Zheng, Q., Xu, H."Assessment of the embryotoxicity of four Chinese herbal extracts using the embryonic stem cell test". Molecular Medicine Reports 12.2 (2015): 2348-2354.
Chicago
Li, L., Cao, F., Su, Z., Zhang, Q., Dai, X., Xiao, X., Huang, Y., Zheng, Q., Xu, H."Assessment of the embryotoxicity of four Chinese herbal extracts using the embryonic stem cell test". Molecular Medicine Reports 12, no. 2 (2015): 2348-2354. https://doi.org/10.3892/mmr.2015.3598
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team