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Article

Neuroprotective effects of brilliant blue G on the brain following traumatic brain injury in rats

  • Authors:
    • Yong‑Chao Wang
    • Ying Cui
    • Jian‑Zhong Cui
    • Li‑Qian Sun
    • Chang‑Meng Cui
    • Hong‑Ao Zhang
    • Hui‑Xing Zhu
    • Ran Li
    • Yan‑Xia Tian
    • Jun‑Ling Gao
  • View Affiliations / Copyright

    Affiliations: Department of Basic Medical Science, Hebei United University, Tangshan, Hebei 063000, P.R. China, Department of Surgery, Tangshan Workers' Hospital, Tangshan, Hebei 063000, P.R. China, Department of Surgery, Hebei Medical University, Shijiazhuang, Hebei 050017, P.R. China
  • Pages: 2149-2154
    |
    Published online on: April 14, 2015
       https://doi.org/10.3892/mmr.2015.3607
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Abstract

The P2X7 inhibitor, brilliant blue G (BBG), has been reported as a neuroprotective drug against a variety of disorders, including neuropathic pain and brain ischemia. Currently, no studies have examined the potential for BBG to provide neuroprotection in animal models of TBI. The aim of the present study was to investigate the neuroprotective effect of BBG on TBI and to determine the underlying mechanisms. The rats were subjected to a diffuse cortical impact injury caused by a modified weight‑drop device, and then divided randomly into three groups: the sham‑operated, BBG treatment and vehicle groups. In the BBG treatment group, 50 mg/kg brilliant blue G (BBG; 100% pure), a highly specific and clinically useful P2X7 antagonist, was administered via the tail vein 15 min prior to or up to 8 h following TBI. The co‑localization of NeuN and protein kinase Cγ (PKCγ) was followed with immunofluorescent staining. The expression of P2X7, PKCγ and inflammatory cytokines was identified by western blot analysis. Wet‑dry weight method was used to evaluate brain edema, and motor function outcome was examined using the neurological severity score. The present study demonstrated that the administration of BBG attenuated TBI‑induced cerebral edema and the associated motor deficits. Following trauma, BBG treatment significantly reduced the levels of PKCγ and interleukin‑1β in the cortex. The results provide in vivo evidence that BBG exerted neuroprotective effects by attenuating brain edema and improving neurological functions via reducing PKCγ and interleukin-1β levels following TBI.
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Copy and paste a formatted citation
Spandidos Publications style
Wang YC, Cui Y, Cui JZ, Sun LQ, Cui CM, Zhang HA, Zhu HX, Li R, Tian YX, Gao JL, Gao JL, et al: Neuroprotective effects of brilliant blue G on the brain following traumatic brain injury in rats. Mol Med Rep 12: 2149-2154, 2015.
APA
Wang, Y., Cui, Y., Cui, J., Sun, L., Cui, C., Zhang, H. ... Gao, J. (2015). Neuroprotective effects of brilliant blue G on the brain following traumatic brain injury in rats. Molecular Medicine Reports, 12, 2149-2154. https://doi.org/10.3892/mmr.2015.3607
MLA
Wang, Y., Cui, Y., Cui, J., Sun, L., Cui, C., Zhang, H., Zhu, H., Li, R., Tian, Y., Gao, J."Neuroprotective effects of brilliant blue G on the brain following traumatic brain injury in rats". Molecular Medicine Reports 12.2 (2015): 2149-2154.
Chicago
Wang, Y., Cui, Y., Cui, J., Sun, L., Cui, C., Zhang, H., Zhu, H., Li, R., Tian, Y., Gao, J."Neuroprotective effects of brilliant blue G on the brain following traumatic brain injury in rats". Molecular Medicine Reports 12, no. 2 (2015): 2149-2154. https://doi.org/10.3892/mmr.2015.3607
Copy and paste a formatted citation
x
Spandidos Publications style
Wang YC, Cui Y, Cui JZ, Sun LQ, Cui CM, Zhang HA, Zhu HX, Li R, Tian YX, Gao JL, Gao JL, et al: Neuroprotective effects of brilliant blue G on the brain following traumatic brain injury in rats. Mol Med Rep 12: 2149-2154, 2015.
APA
Wang, Y., Cui, Y., Cui, J., Sun, L., Cui, C., Zhang, H. ... Gao, J. (2015). Neuroprotective effects of brilliant blue G on the brain following traumatic brain injury in rats. Molecular Medicine Reports, 12, 2149-2154. https://doi.org/10.3892/mmr.2015.3607
MLA
Wang, Y., Cui, Y., Cui, J., Sun, L., Cui, C., Zhang, H., Zhu, H., Li, R., Tian, Y., Gao, J."Neuroprotective effects of brilliant blue G on the brain following traumatic brain injury in rats". Molecular Medicine Reports 12.2 (2015): 2149-2154.
Chicago
Wang, Y., Cui, Y., Cui, J., Sun, L., Cui, C., Zhang, H., Zhu, H., Li, R., Tian, Y., Gao, J."Neuroprotective effects of brilliant blue G on the brain following traumatic brain injury in rats". Molecular Medicine Reports 12, no. 2 (2015): 2149-2154. https://doi.org/10.3892/mmr.2015.3607
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