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Article

Conditionally replicating oncolytic adenoviral vector expressing arresten and tumor necrosis factor-related apoptosis-inducing ligand experimentally suppresses lung carcinoma progression

  • Authors:
    • Shudong Li
    • Zongli Qi
    • Huijin Li
    • Jun Hu
    • Dongyang Wang
    • Xin Wang
    • Zhenzhen Feng
  • View Affiliations / Copyright

    Affiliations: Department of Thoracic Surgery, Shaanxi Provincial People's Hospital, Third Affiliated Hospital, Xi'an Jiaotong University, Xi'an, Shaanxi 710068, P.R. China, Laboratory Center, Shaanxi Provincial People's Hospital, Third Affiliated Hospital, Xi'an Jiaotong University, Xi'an, Shaanxi 710068, P.R. China, Laboratory of Gene Therapy, Department of Biochemistry, College of Life Sciences, Shaanxi Normal University, Xi'an, Shaanxi 710119, P.R. China
  • Pages: 2068-2074
    |
    Published online on: April 16, 2015
       https://doi.org/10.3892/mmr.2015.3624
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Abstract

Current methods of treatment for lung carcinoma are ineffective for the majority of patients. Conditionally replicating adenoviruses (CRAds) represent a potential novel treatment for a number of neoplastic diseases, including lung carcinoma. The present study aimed to investigate the synergistic mechanisms underlying the anti‑angiogenesis gene, arresten, and the apoptosis‑inducing gene, tumor necrosis factor‑related apoptosis‑inducing ligand (TRAIL), in order to evaluate their therapeutic potential in lung cancer. The two genes were expressed by CRAd, which was confirmed using reverse transcription‑polymerase chain reaction and western blotting. In vitro analyses demonstrated that CRAd adenoviruses are capable of selectively inhibiting A549 lung cancer cell growth and replication but not in that of healthy cells. In vivo analyses demonstrated that the infection of A549 cell lines using CRAd armed with the two genes (CRAd‑arresten‑TRAIL) enhanced the tumor inhibition, compared with cells infected with CRAd‑arresten, CRAd‑TRAIL or CRAd, and with the control group. CRAd‑arresten‑TRAIL may therefore be useful in the treatment of lung cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Li S, Qi Z, Li H, Hu J, Wang D, Wang X and Feng Z: Conditionally replicating oncolytic adenoviral vector expressing arresten and tumor necrosis factor-related apoptosis-inducing ligand experimentally suppresses lung carcinoma progression. Mol Med Rep 12: 2068-2074, 2015.
APA
Li, S., Qi, Z., Li, H., Hu, J., Wang, D., Wang, X., & Feng, Z. (2015). Conditionally replicating oncolytic adenoviral vector expressing arresten and tumor necrosis factor-related apoptosis-inducing ligand experimentally suppresses lung carcinoma progression. Molecular Medicine Reports, 12, 2068-2074. https://doi.org/10.3892/mmr.2015.3624
MLA
Li, S., Qi, Z., Li, H., Hu, J., Wang, D., Wang, X., Feng, Z."Conditionally replicating oncolytic adenoviral vector expressing arresten and tumor necrosis factor-related apoptosis-inducing ligand experimentally suppresses lung carcinoma progression". Molecular Medicine Reports 12.2 (2015): 2068-2074.
Chicago
Li, S., Qi, Z., Li, H., Hu, J., Wang, D., Wang, X., Feng, Z."Conditionally replicating oncolytic adenoviral vector expressing arresten and tumor necrosis factor-related apoptosis-inducing ligand experimentally suppresses lung carcinoma progression". Molecular Medicine Reports 12, no. 2 (2015): 2068-2074. https://doi.org/10.3892/mmr.2015.3624
Copy and paste a formatted citation
x
Spandidos Publications style
Li S, Qi Z, Li H, Hu J, Wang D, Wang X and Feng Z: Conditionally replicating oncolytic adenoviral vector expressing arresten and tumor necrosis factor-related apoptosis-inducing ligand experimentally suppresses lung carcinoma progression. Mol Med Rep 12: 2068-2074, 2015.
APA
Li, S., Qi, Z., Li, H., Hu, J., Wang, D., Wang, X., & Feng, Z. (2015). Conditionally replicating oncolytic adenoviral vector expressing arresten and tumor necrosis factor-related apoptosis-inducing ligand experimentally suppresses lung carcinoma progression. Molecular Medicine Reports, 12, 2068-2074. https://doi.org/10.3892/mmr.2015.3624
MLA
Li, S., Qi, Z., Li, H., Hu, J., Wang, D., Wang, X., Feng, Z."Conditionally replicating oncolytic adenoviral vector expressing arresten and tumor necrosis factor-related apoptosis-inducing ligand experimentally suppresses lung carcinoma progression". Molecular Medicine Reports 12.2 (2015): 2068-2074.
Chicago
Li, S., Qi, Z., Li, H., Hu, J., Wang, D., Wang, X., Feng, Z."Conditionally replicating oncolytic adenoviral vector expressing arresten and tumor necrosis factor-related apoptosis-inducing ligand experimentally suppresses lung carcinoma progression". Molecular Medicine Reports 12, no. 2 (2015): 2068-2074. https://doi.org/10.3892/mmr.2015.3624
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