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Article

Rho-associated protein kinase inhibitor, Y-27632, significantly enhances cell adhesion and induces a delay in G1 to S phase transition in rabbit corneal endothelial cells

  • Authors:
    • Yu‑Mei Diao
    • Jing Hong
  • View Affiliations / Copyright

    Affiliations: Department of Ophthalmology, Peking University Third Hospital, Haidian, Beijing 100191, P.R. China
  • Pages: 1951-1956
    |
    Published online on: April 16, 2015
       https://doi.org/10.3892/mmr.2015.3628
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Abstract

Human corneal endothelial cells are a non-proliferative cell type. As a result of the increase in corneal endothelium disease, increasing numbers of studies have been conducted in order to promote corneal endothelial cell proliferation. The aim of the present study was to investigate the proliferative effects of Rho‑associated protein kinase inhibitor, Y‑27632, on rabbit corneal endothelial cells (rCECs). Y‑27632 (1, 10 or 30 µM) was added at two different time points to two groups of rCECs. The first group received Y‑27632 when rCECs were initially plated, and the second following 72 h of cell growth. Cell morphology and cell adhesion ratios were subsequently observed using light microscopy. A cell counting kit was used to measure the number of viable cells that adhered to culture plates. Cell cycle transitions and levels of Annexin V‑positive apoptotic cells were detected using flow cytometry. Cells treated with 1 µM Y‑27632 and 10 µM Y‑27632 retained their cell shape. At a concentration of 30 µM Y‑27632, the cell shape became irregular. Cell adhesion ratios, in 1 µM Y‑27632 (36.84%), 10 µM Y‑27632 (84.21%) and 30 µM Y‑27632 (84.21%) were higher than the adhesion ratio in the control group (P<0.01). The optical densities of rCECs treated with 10 µM or 30 µM Y‑27632 following 72 h of cell growth was less than that of the control cells (P<0.01), but higher than that of cells which received Y‑27632 at the time of plating (P<0.01). Flow cytometry results also demonstrated that there was a delay in G1 to S phase cell cycle progression in rCECs following administration of 10 µM Y‑27632 (P<0.01). Cell apoptosis was inhibited when 10 µM Y‑27632 was added, at the time of cell plating, as well as when added following 72 h of cell growth (P<0.01). At a concentration of 10 µM Y‑27632, there was an improvement in cell adhesion and an inhibition of the cell cycle in rabbit corneal endothelial cells. In conclusion, Y‑27632 has different effects on rCECs when administered at varying concentrations and at particular stages of cell growth.
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Copy and paste a formatted citation
Spandidos Publications style
Diao YM and Hong J: Rho-associated protein kinase inhibitor, Y-27632, significantly enhances cell adhesion and induces a delay in G1 to S phase transition in rabbit corneal endothelial cells. Mol Med Rep 12: 1951-1956, 2015.
APA
Diao, Y., & Hong, J. (2015). Rho-associated protein kinase inhibitor, Y-27632, significantly enhances cell adhesion and induces a delay in G1 to S phase transition in rabbit corneal endothelial cells. Molecular Medicine Reports, 12, 1951-1956. https://doi.org/10.3892/mmr.2015.3628
MLA
Diao, Y., Hong, J."Rho-associated protein kinase inhibitor, Y-27632, significantly enhances cell adhesion and induces a delay in G1 to S phase transition in rabbit corneal endothelial cells". Molecular Medicine Reports 12.2 (2015): 1951-1956.
Chicago
Diao, Y., Hong, J."Rho-associated protein kinase inhibitor, Y-27632, significantly enhances cell adhesion and induces a delay in G1 to S phase transition in rabbit corneal endothelial cells". Molecular Medicine Reports 12, no. 2 (2015): 1951-1956. https://doi.org/10.3892/mmr.2015.3628
Copy and paste a formatted citation
x
Spandidos Publications style
Diao YM and Hong J: Rho-associated protein kinase inhibitor, Y-27632, significantly enhances cell adhesion and induces a delay in G1 to S phase transition in rabbit corneal endothelial cells. Mol Med Rep 12: 1951-1956, 2015.
APA
Diao, Y., & Hong, J. (2015). Rho-associated protein kinase inhibitor, Y-27632, significantly enhances cell adhesion and induces a delay in G1 to S phase transition in rabbit corneal endothelial cells. Molecular Medicine Reports, 12, 1951-1956. https://doi.org/10.3892/mmr.2015.3628
MLA
Diao, Y., Hong, J."Rho-associated protein kinase inhibitor, Y-27632, significantly enhances cell adhesion and induces a delay in G1 to S phase transition in rabbit corneal endothelial cells". Molecular Medicine Reports 12.2 (2015): 1951-1956.
Chicago
Diao, Y., Hong, J."Rho-associated protein kinase inhibitor, Y-27632, significantly enhances cell adhesion and induces a delay in G1 to S phase transition in rabbit corneal endothelial cells". Molecular Medicine Reports 12, no. 2 (2015): 1951-1956. https://doi.org/10.3892/mmr.2015.3628
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