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Article

Aberrant methylation of the GCK gene body is associated with the risk of essential hypertension

  • Authors:
    • Rui Fan
    • Wei‑Jie Wang
    • Qi-Long Zhong
    • Shi-Wei Duan
    • Xu‑Ting Xu
    • Ling‑Mei Hao
    • Jing Zhao
    • Li-Na Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Preventive Medicine, Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, Ningbo, Zhejiang 315211, P.R. China, Department of General Medicine, Ningbo Baizhang Street Community Health Service Center, Ningbo, Zhejiang 315211, P.R. China , Clinical Laboratory, The Seventh Hospital of Ningbo, Ningbo, Zhejiang 315211, P.R. China
  • Pages: 2390-2394
    |
    Published online on: April 16, 2015
       https://doi.org/10.3892/mmr.2015.3631
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Abstract

Essential hypertension (EH) is commonly accompanied by a dysfunction of glucose metabolism. Glucokinase (GCK) is a key enzyme involved in glucose metabolism. The aim of the present study was to investigate whether GCK gene‑body methylation contributed to the risk of EH. A total of 47 patients with EH and 47 age‑matched controls were recruited for methylation research in the current study. GCK gene‑body methylation was measured using bisulphite pyrosequencing technology. DNA methylation levels were closely correlated among CpG1, CpG2 and CpG3 (r>0.70; P<0.001), in contrast with a weaker correlation between CpG4 and the preceding three CpGs (r<0.3 or r=1; P>0.05). Significantly lower CpG1‑3 methylation (cases vs. controls, 49.13±5.72 vs. 53.49±7.53%; adjusted P=0.006) and significantly higher CpG4 methylation (cases vs. controls, 46.34±6.48 vs. 34.74±12.73%; adjusted P=0.002) were observed in patients with EH. The present study indicated that aberrant methylation of the GCK gene body was significantly associated with the risk of EH in the population assessed. The discrepancies between CpG1‑3 and CpG4 methylation may suggest distinct roles for each of them in the determination of the risk of EH.
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Copy and paste a formatted citation
Spandidos Publications style
Fan R, Wang WJ, Zhong Q, Duan S, Xu XT, Hao LM, Zhao J and Zhang L: Aberrant methylation of the GCK gene body is associated with the risk of essential hypertension. Mol Med Rep 12: 2390-2394, 2015.
APA
Fan, R., Wang, W., Zhong, Q., Duan, S., Xu, X., Hao, L. ... Zhang, L. (2015). Aberrant methylation of the GCK gene body is associated with the risk of essential hypertension. Molecular Medicine Reports, 12, 2390-2394. https://doi.org/10.3892/mmr.2015.3631
MLA
Fan, R., Wang, W., Zhong, Q., Duan, S., Xu, X., Hao, L., Zhao, J., Zhang, L."Aberrant methylation of the GCK gene body is associated with the risk of essential hypertension". Molecular Medicine Reports 12.2 (2015): 2390-2394.
Chicago
Fan, R., Wang, W., Zhong, Q., Duan, S., Xu, X., Hao, L., Zhao, J., Zhang, L."Aberrant methylation of the GCK gene body is associated with the risk of essential hypertension". Molecular Medicine Reports 12, no. 2 (2015): 2390-2394. https://doi.org/10.3892/mmr.2015.3631
Copy and paste a formatted citation
x
Spandidos Publications style
Fan R, Wang WJ, Zhong Q, Duan S, Xu XT, Hao LM, Zhao J and Zhang L: Aberrant methylation of the GCK gene body is associated with the risk of essential hypertension. Mol Med Rep 12: 2390-2394, 2015.
APA
Fan, R., Wang, W., Zhong, Q., Duan, S., Xu, X., Hao, L. ... Zhang, L. (2015). Aberrant methylation of the GCK gene body is associated with the risk of essential hypertension. Molecular Medicine Reports, 12, 2390-2394. https://doi.org/10.3892/mmr.2015.3631
MLA
Fan, R., Wang, W., Zhong, Q., Duan, S., Xu, X., Hao, L., Zhao, J., Zhang, L."Aberrant methylation of the GCK gene body is associated with the risk of essential hypertension". Molecular Medicine Reports 12.2 (2015): 2390-2394.
Chicago
Fan, R., Wang, W., Zhong, Q., Duan, S., Xu, X., Hao, L., Zhao, J., Zhang, L."Aberrant methylation of the GCK gene body is associated with the risk of essential hypertension". Molecular Medicine Reports 12, no. 2 (2015): 2390-2394. https://doi.org/10.3892/mmr.2015.3631
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