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Article

Salvianolic acid B improves bone marrow-derived mesenchymal stem cell differentiation into alveolar epithelial cells type I via Wnt signaling

  • Authors:
    • Peng Gao
    • Jingxian Yang
    • Xi Gao
    • Dan Xu
    • Dongge Niu
    • Jinglin Li
    • Qingping Wen
  • View Affiliations / Copyright

    Affiliations: Department of Anesthesiology, Dalian Medical University, Dalian, Liaoning 116044, P.R. China, Department of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian, Liaoning 116600, P.R. China, Department of Anesthesiology, First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116011, P.R. China
  • Pages: 1971-1976
    |
    Published online on: April 16, 2015
       https://doi.org/10.3892/mmr.2015.3632
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Abstract

Acute lung injury (ALI) is among the most common causes of mortality in intensive care units. Previous studies have suggested that bone marrow‑derived mesenchymal stem cells (BMSCs) may attenuate pulmonary edema. In addition, alveolar epithelial cells type Ⅰ (ATⅠ) are involved in reducing the alveolar edema in response to ALI. However, the mechanism involved in improving the efficiency of differentiation of MSCs into ATⅠ remains to be elucidated. In the present study, the effect of salvianolic acid B (Sal B) on the differentiation of BMSCs into ATⅠ and the activities of the Wnt signaling pathways were investigated. The BMSCs were supplemented with conditioned medium (CM). The groups were as follows: i) CM group: BMSCs were supplemented with CM; ii) lithium chloride (LiCl) group: BMSCs were supplemented with CM and 5 mM LiCl; iii) Sal B group: BMSCs were supplemented with CM and 10 mM Sal B. The samples were collected and assessed on days 7 and 14. It was revealed that aquaporin (AQP)‑5 and T1α were expressed in BMSCs, and induction with LiCl or Sal B increased the expression of AQP‑5 and T1α. Furthermore, the Wnt‑1 and Wnt‑3a signaling pathways were activated during the differentiation of BMSCs into ATⅠ. In conclusion, it was suggested that the promotive effects of Sal B on the differentiation of BMSCs into ATⅠ occurred through the activation of Wnt signaling pathways.
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Copy and paste a formatted citation
Spandidos Publications style
Gao P, Yang J, Gao X, Xu D, Niu D, Li J and Wen Q: Salvianolic acid B improves bone marrow-derived mesenchymal stem cell differentiation into alveolar epithelial cells type I via Wnt signaling. Mol Med Rep 12: 1971-1976, 2015.
APA
Gao, P., Yang, J., Gao, X., Xu, D., Niu, D., Li, J., & Wen, Q. (2015). Salvianolic acid B improves bone marrow-derived mesenchymal stem cell differentiation into alveolar epithelial cells type I via Wnt signaling. Molecular Medicine Reports, 12, 1971-1976. https://doi.org/10.3892/mmr.2015.3632
MLA
Gao, P., Yang, J., Gao, X., Xu, D., Niu, D., Li, J., Wen, Q."Salvianolic acid B improves bone marrow-derived mesenchymal stem cell differentiation into alveolar epithelial cells type I via Wnt signaling". Molecular Medicine Reports 12.2 (2015): 1971-1976.
Chicago
Gao, P., Yang, J., Gao, X., Xu, D., Niu, D., Li, J., Wen, Q."Salvianolic acid B improves bone marrow-derived mesenchymal stem cell differentiation into alveolar epithelial cells type I via Wnt signaling". Molecular Medicine Reports 12, no. 2 (2015): 1971-1976. https://doi.org/10.3892/mmr.2015.3632
Copy and paste a formatted citation
x
Spandidos Publications style
Gao P, Yang J, Gao X, Xu D, Niu D, Li J and Wen Q: Salvianolic acid B improves bone marrow-derived mesenchymal stem cell differentiation into alveolar epithelial cells type I via Wnt signaling. Mol Med Rep 12: 1971-1976, 2015.
APA
Gao, P., Yang, J., Gao, X., Xu, D., Niu, D., Li, J., & Wen, Q. (2015). Salvianolic acid B improves bone marrow-derived mesenchymal stem cell differentiation into alveolar epithelial cells type I via Wnt signaling. Molecular Medicine Reports, 12, 1971-1976. https://doi.org/10.3892/mmr.2015.3632
MLA
Gao, P., Yang, J., Gao, X., Xu, D., Niu, D., Li, J., Wen, Q."Salvianolic acid B improves bone marrow-derived mesenchymal stem cell differentiation into alveolar epithelial cells type I via Wnt signaling". Molecular Medicine Reports 12.2 (2015): 1971-1976.
Chicago
Gao, P., Yang, J., Gao, X., Xu, D., Niu, D., Li, J., Wen, Q."Salvianolic acid B improves bone marrow-derived mesenchymal stem cell differentiation into alveolar epithelial cells type I via Wnt signaling". Molecular Medicine Reports 12, no. 2 (2015): 1971-1976. https://doi.org/10.3892/mmr.2015.3632
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