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Article

Puerarin enhances proliferation and osteoblastic differentiation of human bone marrow stromal cells via a nitric oxide/cyclic guanosine monophosphate signaling pathway

  • Authors:
    • Haihong Lv
    • Tuanjie Che
    • Xulei Tang
    • Lijuan Liu
    • Jianguo Cheng
  • View Affiliations / Copyright

    Affiliations: Department of Endocrinology and Metabolism, The First Hospital of Lanzhou University, Lanzhou, Gansu 730000, P.R. China, Baiyuan Gene Technology Co. Ltd, Lanzhou, Gansu 730000, P.R. China
  • Pages: 2283-2290
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    Published online on: April 20, 2015
       https://doi.org/10.3892/mmr.2015.3647
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Abstract

Puerarin, a major active isoflavone extracted from the Traditional Chinese Medicine Radix Puerariae, has been studied for its comprehensive biological effects. However, to date, its effect on bone formation and the underlying mechanism of action have not been well investigated. The present study investigated the effect of puerarin on cell proliferation and osteoblastic maturation in cultured human bone marrow stromal cells (hBMSC) in vitro. Puerarin (2.5‑100 µM) increased hBMSC growth in a dose‑dependent manner, as indicated by an MTT assay, and stimulated osteoblastic maturation as indicated by assessment of alkaline phosphatase (ALP) activity, as well as calcium deposition into the extracellular matrix detected by alizarin red S staining. Furthermore, polymerase chain reaction analysis showed that the expression of osteoblastic markers, including Runt-related transcription factor 2/core-binding factor alpha 1, osterix and osteocalcin, were increased in hBMSCs following incubation with puerarin. Further experiments indicated that puerarin increased the nitric oxide (NO) production and cyclic guanosine monophosphate (cGMP) content in hBMSCs. The effects of puerarin were mimicked by 17β‑estrodiol (10‑8 M) and were abolished in the presence of estrogen receptor antagonist ICI182780 (10‑7 M). A NO synthase inhibitor, Nx‑nitro‑L‑arginine methylester (6x10‑3 M), significantly attenuated puerarin‑induced increases in NO production and cGMP content, in parallel with a reduction of cell proliferation and osteoblastic differentiation as well as the expression of osteoblastic markers. These results suggested that puerarin may prevent osteoporosis by exerting stimulatory effects on bone formation and the NO/cGMP pathway, which has an important role in puerarin‑induced hBMSC proliferation and osteoblastic differentiation.
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Copy and paste a formatted citation
Spandidos Publications style
Lv H, Che T, Tang X, Liu L and Cheng J: Puerarin enhances proliferation and osteoblastic differentiation of human bone marrow stromal cells via a nitric oxide/cyclic guanosine monophosphate signaling pathway. Mol Med Rep 12: 2283-2290, 2015.
APA
Lv, H., Che, T., Tang, X., Liu, L., & Cheng, J. (2015). Puerarin enhances proliferation and osteoblastic differentiation of human bone marrow stromal cells via a nitric oxide/cyclic guanosine monophosphate signaling pathway. Molecular Medicine Reports, 12, 2283-2290. https://doi.org/10.3892/mmr.2015.3647
MLA
Lv, H., Che, T., Tang, X., Liu, L., Cheng, J."Puerarin enhances proliferation and osteoblastic differentiation of human bone marrow stromal cells via a nitric oxide/cyclic guanosine monophosphate signaling pathway". Molecular Medicine Reports 12.2 (2015): 2283-2290.
Chicago
Lv, H., Che, T., Tang, X., Liu, L., Cheng, J."Puerarin enhances proliferation and osteoblastic differentiation of human bone marrow stromal cells via a nitric oxide/cyclic guanosine monophosphate signaling pathway". Molecular Medicine Reports 12, no. 2 (2015): 2283-2290. https://doi.org/10.3892/mmr.2015.3647
Copy and paste a formatted citation
x
Spandidos Publications style
Lv H, Che T, Tang X, Liu L and Cheng J: Puerarin enhances proliferation and osteoblastic differentiation of human bone marrow stromal cells via a nitric oxide/cyclic guanosine monophosphate signaling pathway. Mol Med Rep 12: 2283-2290, 2015.
APA
Lv, H., Che, T., Tang, X., Liu, L., & Cheng, J. (2015). Puerarin enhances proliferation and osteoblastic differentiation of human bone marrow stromal cells via a nitric oxide/cyclic guanosine monophosphate signaling pathway. Molecular Medicine Reports, 12, 2283-2290. https://doi.org/10.3892/mmr.2015.3647
MLA
Lv, H., Che, T., Tang, X., Liu, L., Cheng, J."Puerarin enhances proliferation and osteoblastic differentiation of human bone marrow stromal cells via a nitric oxide/cyclic guanosine monophosphate signaling pathway". Molecular Medicine Reports 12.2 (2015): 2283-2290.
Chicago
Lv, H., Che, T., Tang, X., Liu, L., Cheng, J."Puerarin enhances proliferation and osteoblastic differentiation of human bone marrow stromal cells via a nitric oxide/cyclic guanosine monophosphate signaling pathway". Molecular Medicine Reports 12, no. 2 (2015): 2283-2290. https://doi.org/10.3892/mmr.2015.3647
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