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Activation of novel estrogen receptor GPER results in inhibition of cardiocyte apoptosis and cardioprotection

  • Authors:
    • Wan‑Li Li
    • Wei Xiang
    • Ye Ping
  • View Affiliations / Copyright

    Affiliations: Department of Cardiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, P.R. China, Department of Cardiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430022, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 2425-2430
    |
    Published online on: April 24, 2015
       https://doi.org/10.3892/mmr.2015.3674
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Abstract

Several studies have recently demonstrated that G protein‑coupled estrogen receptor (GPER) 30 directly binds to estrogen and mediates its action. The aim of the present study was to investigate the effects of GPER on cardiocyte apoptosis following ischemia/reperfusion injury (MIRI) in H9C2 myocardial cells. H9C2 cells were treated with a specific GPER agonist (G1), 17β‑estradiol (E2) or the vehicle. The cells were subjected to 20 min of myocardial ischemia followed by 120 min of reperfusion. They were then randomly assigned to three experimental groups: Control, G1, E2. B‑cell lymphoma 2 (Bcl‑2) and Bcl‑2 associated X (Bax) levels were measured, Hoechst 33258 staining was performed to assess apoptosis, and superoxide dismutase (SOD), tumor necrosis factor (TNF)‑α and adenosine triphosphatase (ATPase) levels were determined. To test the specificity of G1, GPER‑knockout cells were treated with G1 and analyzed as stated above. Compared with the vehicle‑treated groups, G1 and E2‑treated groups exhibited elevated Bcl‑2 levels, decreased Bax levels and cell apoptosis, significantly increased SOD and ATP levels and decreased TNF‑α levels following ischemia‑reperfusion. However, G1 had no evident effects on the GPER‑knockout cells. In conclusion, the present study suggested that GPER activation provided a cardioprotective effect following ischemia‑reperfusion by inhibiting cardiocyte apoptosis.
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1 

Murphy E and Steenbergen C: Cardioprotection in females: a role for nitric oxide and altered gene expression. Heart Fail Rev. 12:293–300. 2007. View Article : Google Scholar : PubMed/NCBI

2 

Grohé C, Kahlert S, Löbbert K, et al: Cardiac myocytes and fibroblasts contain functional estrogen receptors. FEBS Lett. 416:107–112. 1997. View Article : Google Scholar : PubMed/NCBI

3 

Lizotte E, Grandy SA, Tremblay A, Allen BG and Fiset C: Expression, distribution and regulation of sex steroid hormone receptors in mouse heart. Cell Physiol Biochem. 23:75–86. 2009. View Article : Google Scholar : PubMed/NCBI

4 

Deschamps AM and Murphy E: Activation of a novel estrogen receptor, GPER, is cardioprotective in male and female rats. Am J Physiol Heart Circ Physiol. 297:H1806–H1813. 2009. View Article : Google Scholar : PubMed/NCBI

5 

Bopassa JC, Eghbali M, Toro L and Stefani E: A novel estrogen receptor GPER inhibits mitochondria permeability transition pore opening and protects the heart against ischemia-reperfusion injury. Am J Physiol. 98:H16–H23. 2010.

6 

Albanito L, Madeo A, Lappano R, et al: G protein-coupled receptor 30 (GPR30) mediates gene expression changes and growth response to 17beta-estradiol and selective GPR30 ligand G-1 in ovarian cancer cells. Cancer Res. 67:1859–1866. 2007. View Article : Google Scholar : PubMed/NCBI

7 

Carmeci C, Thompson DA, Ring HZ, Francke U and Weigel RJ: Identification of a gene (GPR30) with homology to the G-protein-coupled receptor superfamily associated with estrogen receptor expression in breast cancer. Genomics. 45:607–617. 1997. View Article : Google Scholar

8 

Otto C, Rohde-Schulz B, Schwarz G, et al: G protein-coupled receptor 30 localizes to the endoplasmic reticulum and is not activated by estradiol. Endocrinology. 149:4846–4856. 2008. View Article : Google Scholar : PubMed/NCBI

9 

Shanmuganathan S, Hausenloy DJ, Duchen MR and Yellon DM: Mitochondrial permeability transition pore as a target for cardioprotection in the human heart. Am J Physiol Heart Circ Physiol. 289:H237–H242. 2005. View Article : Google Scholar : PubMed/NCBI

10 

Bopassa JC, Eghbali M, Toro L and Stefani E: A novel estrogen receptor GPER inhibits mitochondria permeability transition pore opening and protects the heart against ischemia-reperfusion injury. Am J Physiol Heart Circ Physiol. 298:H16–H23. 2010. View Article : Google Scholar :

11 

Yuan SM and Jing H: Insights into the monomers and single drugs of Chinese herbal medicine on myocardial preservation. Afr J Tradit Complement Altern Med. 8:104–127. 2011.

12 

Hu BJ, Chen JG, Xu XH, Chen Yc and Zhu JZ: Experimental study on the cardiomyocyte apoptosis in the early myocardial ischemia. Chin J Forens Med. 17:349–351. 2001.

13 

Deschamps AM and Murphy E: Activation of a novel estrogen receptor, GPER, is cardioprotective in male and female rats. Am J Physiol Heart Circ Physiol. 297:H1806–H1813. 2009. View Article : Google Scholar : PubMed/NCBI

14 

Bologa CG, Revankar CM, Young SM, et al: Virtual and biomolecular screening converge on a selective agonist for GPR30. Nat Chem Biol. 2:207–212. 2006. View Article : Google Scholar : PubMed/NCBI

15 

Revankar CM, Cimino DF, Sklar LA, Arterburn JB and Prossnitz ER: A transmembrane intracellular estrogen receptor mediates rapid cell signaling. Science. 307:1625–1630. 2005. View Article : Google Scholar : PubMed/NCBI

16 

Filardo E, Quinn J, Pang Y, et al: Activation of the novel estrogen receptor G protein-coupled receptor 30 (GPR30) at the plasma membrane. Endocrinology. 148:3236–3245. 2007. View Article : Google Scholar : PubMed/NCBI

17 

Matsuda K, Sakamoto H, Mori H, et al: Expression and intracellular distribution of the G protein-coupled receptor 30 in rat hippocampal formation. Neurosci Lett. 441:94–99. 2008. View Article : Google Scholar : PubMed/NCBI

18 

Haas E, Bhattacharya I, Brailoiu E, et al: Regulatory role of G protein-coupled estrogen receptor for vascular function and obesity. Circ Res. 104:288–291. 2009. View Article : Google Scholar : PubMed/NCBI

19 

Bopassa JC, Eghbali M, Toro L and Stefani E: A novel estrogen receptor GPER inhibits mitochondria permeability transition pore opening and protects the heart against ischemia-reperfusion injury. Am J Physiol Heart Circ Physiol. 298:H16–H23. 2010. View Article : Google Scholar :

20 

Yellon DM and Hausenloy DJ: Myocardial reperfusion injury. N Engl J Med. 357:1121–1135. 2007. View Article : Google Scholar : PubMed/NCBI

21 

Ishihara Y and Shimamoto N: Sulfaphenazole attenuates myocardial cell apoptosis accompanied with cardiac ischemia-reperfusion by suppressing the expression of BimEL and Noxa. J Pharmacol Sci. 119:251–259. 2012. View Article : Google Scholar : PubMed/NCBI

22 

Liesa M, Luptak I, Qin F, et al: Mitochondrial transporter ATP binding cassette mitochondrial erythroid is a novel gene required for cardiac recovery after ischemia/reperfusion. Circulation. 124:806–813. 2011. View Article : Google Scholar : PubMed/NCBI

23 

Hansson GK: Immune and inflammatory mechanisms in the development of atherosclerosis. Br Heart J. 69(Suppl): S38–S41. 1993. View Article : Google Scholar : PubMed/NCBI

24 

Herskowitz A, Choi S, Ansari AA and Wesselingh S: Cytokine mRNA expression in postischemic/reperfusion myocardium. Am J Pathol. 146:419–428. 1995.PubMed/NCBI

25 

Feldman AM, Combes A, Wagner D, Kadakomi T, Kubota T, et al: The role of tumor necrosis factor in the pathophysiology of heart failure. J Am Coll Cardiol. 35:537–544. 2000. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Li WL, Xiang W and Ping Y: Activation of novel estrogen receptor GPER results in inhibition of cardiocyte apoptosis and cardioprotection. Mol Med Rep 12: 2425-2430, 2015.
APA
Li, W., Xiang, W., & Ping, Y. (2015). Activation of novel estrogen receptor GPER results in inhibition of cardiocyte apoptosis and cardioprotection. Molecular Medicine Reports, 12, 2425-2430. https://doi.org/10.3892/mmr.2015.3674
MLA
Li, W., Xiang, W., Ping, Y."Activation of novel estrogen receptor GPER results in inhibition of cardiocyte apoptosis and cardioprotection". Molecular Medicine Reports 12.2 (2015): 2425-2430.
Chicago
Li, W., Xiang, W., Ping, Y."Activation of novel estrogen receptor GPER results in inhibition of cardiocyte apoptosis and cardioprotection". Molecular Medicine Reports 12, no. 2 (2015): 2425-2430. https://doi.org/10.3892/mmr.2015.3674
Copy and paste a formatted citation
x
Spandidos Publications style
Li WL, Xiang W and Ping Y: Activation of novel estrogen receptor GPER results in inhibition of cardiocyte apoptosis and cardioprotection. Mol Med Rep 12: 2425-2430, 2015.
APA
Li, W., Xiang, W., & Ping, Y. (2015). Activation of novel estrogen receptor GPER results in inhibition of cardiocyte apoptosis and cardioprotection. Molecular Medicine Reports, 12, 2425-2430. https://doi.org/10.3892/mmr.2015.3674
MLA
Li, W., Xiang, W., Ping, Y."Activation of novel estrogen receptor GPER results in inhibition of cardiocyte apoptosis and cardioprotection". Molecular Medicine Reports 12.2 (2015): 2425-2430.
Chicago
Li, W., Xiang, W., Ping, Y."Activation of novel estrogen receptor GPER results in inhibition of cardiocyte apoptosis and cardioprotection". Molecular Medicine Reports 12, no. 2 (2015): 2425-2430. https://doi.org/10.3892/mmr.2015.3674
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