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August-2015 Volume 12 Issue 2

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Article

Anti‑angiogenic effects of resveratrol in combination with 5‑fluorouracil on B16 murine melanoma cells

  • Authors:
    • Sol Hwa Lee
    • Bong Seong Koo
    • Song Yi Park
    • Young Min Kim
  • View Affiliations / Copyright

    Affiliations: Department of Biological Sciences and Biotechnology, College of Life Science and Nanotechnology, Hannam University, Yusung‑gu, Daejeon 305‑811, Republic of Korea
  • Pages: 2777-2783
    |
    Published online on: April 24, 2015
       https://doi.org/10.3892/mmr.2015.3675
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Abstract

5-Fluorouracil (5-FU) has been used as a chemotherapeutic drug for various types of cancer, although the development of resistance remains a major limitation for its use in clinical settings. In the present study, the anti‑angiogenic effects of resveratrol and 5‑FU either alone or in combination were examined in a B16 murine melanoma model. Co‑treatment using resveratrol and 5‑FU inhibited cell proliferation more efficiently compared with use of either drug alone and the antiproliferative effect coincided with changes in the expression levels of AMP‑activated protein kinase (AMPK), cyclooxygenase‑2, vasodilator‑stimulated phosphoprotein (VASP) and vascular endothelial growth factor (VEGF). Furthermore, co‑treatment with resveratrol and 5‑FU reduced tumor growth compared with that in the control group and this growth‑inhibitory effect was associated with changes in the expression levels of AMPK, VASP and VEGF. Immunohistochemical staining for angiogenesis demonstrated that co‑treatment with resveratrol and 5‑FU reduced the number of microvascular vessels compared with that in the control group. These results suggested that co‑treatment with resveratrol and 5‑FU suppressed cell growth and angiogenesis in B16 murine melanoma tumors.
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1 

Belguendouz L, Fremont L and Linard A: Resveratrol inhibits metal ion-dependent and independent peroxidation of porcine low-density lipoproteins. Biochem Pharmacol. 53:1347–1355. 1997. View Article : Google Scholar : PubMed/NCBI

2 

Emmett MS, Dewing D and Pritchard-Jones RO: Angiogenesis and melanoma - from basic science to clinical trials. Am J Cancer Res. 1:852–868. 2011.PubMed/NCBI

3 

Garvin S, Ollinger K and Dabrosin C: Resveratrol induces apoptosis and inhibits angiogenesiss in human breast cancer xenografts in vivo. Cancer Lett. 231:113–122. 2006. View Article : Google Scholar

4 

Hwang JT, Ha J and Park OJ: Combination of 5-fluorouracil and genistein induces apoptosis synergistically in chemo-resistant cancer cells through the modulation of AMPK and COX-2 signaling pathways. Biochem Biophys Res Commun. 332:433–440. 2005. View Article : Google Scholar : PubMed/NCBI

5 

Cao Z, Fang J, Xia C, Shi X and Jiang BH: trans-3,4,5′-trihydroxystibene inhibits hypoxia-inducible factor 1alpha and vascular endothelial growth factor expression in human ovarian cancer cells. Clin Cancer Res. 10:5253–5263. 2004. View Article : Google Scholar : PubMed/NCBI

6 

Kimura Y and Okuda H: Resveratrol isolated from Polygonumcuspidatum root prevents tumor growth and metastasis to lung and tumor-induced neovascularization in Lewis lung carcinoma-bearing mice. J Nutr. 131:1844–1849. 2001.PubMed/NCBI

7 

Bråkenhielm E, Cao R and Cao Y: Suppression of angiogenesis, tumor growth, and wound healing by resveratrol, a natural compound in red wine and grapes. FASEB J. 15:1798–1800. 2001.PubMed/NCBI

8 

Borralho PM, Moreira da Silva IB, Aranha MM, et al: Inhibition of Fas expression by RNAi modulates 5-fluorouracil-induced-apoptosis in HCT116 cells expressing wild-type p53. Biochim Biophys Acta. 1772:40–47. 2007. View Article : Google Scholar

9 

Ortiz R, Prados J, Melguizo C, et al: 5-Fluorouracil-loaded poly (ε-caprolactone) nanoparticles combined with phage E gene therapy as a new strategy against colon cancer. Int J Nanomedicine. 7:195–107. 2012.

10 

Wu SL, Sun ZJ, Yu L, et al: Effect of resveratrol and in combination with 5-FU on murine liver cancer. World J Gastroenterol. 10:3048–3052. 2004.PubMed/NCBI

11 

Subbaramaiah K and Dannenberg AJ: Cyclooxygenase 2: a moleculartarget for cancer prevention and treatment. Trends Pharmacol Sci. 24:96–102. 2003. View Article : Google Scholar : PubMed/NCBI

12 

Xiao X, Shi D, Liu L, et al: Quercetin suppresses cyclooxygenase-2 expression and angiogenesis through inactivation of P300 signaling. PLoS One. 6:e229342011. View Article : Google Scholar : PubMed/NCBI

13 

Greenhough A, Smartt HJ, Moore AE, et al: The COX-2/PGE2 pathway: key roles in the hallmarks of cancer and adaptation to the tumour microenvironment. Carcinogenesis. 30:377–386. 2009. View Article : Google Scholar : PubMed/NCBI

14 

Yao L, Liu F, Hong L, et al: The function and mechanism of COX-2 in angiogenesis of gastric cancer cells. J Exp Clin Cancer Res. 30:2011. View Article : Google Scholar : PubMed/NCBI

15 

Zykova TA, Zhu F, Zhai X, et al: Resveratrol directly targets COX-2 to inhibit carcinogenesis. Mol Carcinog. 47:797–805. 2008. View Article : Google Scholar : PubMed/NCBI

16 

Kim HN, Kim H, Kong JM, et al: Vitamin C down-regulatesVEGF production in B16F10 murine melanoma cells via the suppression of p42/44 MAPK activation. J Cell Biochem. 112:894–901. 2011. View Article : Google Scholar : PubMed/NCBI

17 

Wu G, Luo J, Rana JS, et al: Involvement of COX-2 in VEGF-induced angiogenesis via P38 and JNK pathways in vascular endothelial cells. Cardiovasc Res. 69:512–519. 2006. View Article : Google Scholar

18 

Kumar B, Chile SA, Ray KB, et al: VEGF-C differentially regulates VEGF-A expression in ocular and cancer cells; promotes angiogenesis via RhoA mediated pathway. Angiogenesis. 14:371–380. 2011. View Article : Google Scholar : PubMed/NCBI

19 

Kim KJ, Li B, Winer J, et al: Inhibition of vascular endothelial growth factor-induced angiogenesis suppresses tumour growth in vivo. Nature. 362:841–844. 1993. View Article : Google Scholar : PubMed/NCBI

20 

Price CJ and Brindle NP: Vasodilator-stimulated phosphoprotein is involved in stress-fiber and membrane ruffle formation in endothelial cells. Arterioscler Thromb Vasc Biol. 20:2051–2056. 2000. View Article : Google Scholar : PubMed/NCBI

21 

Philippar U, Roussos ET, Oser M, et al: A Mena invasion isoform potentiates EGF-induced carcinoma cell invasion and metastasis. Dev Cell. 15:813–828. 2008. View Article : Google Scholar : PubMed/NCBI

22 

Amano H, Hayashi I, Endo H, et al: Host prostaglandin E(2)-EP3 signaling regulates tumor-associated angiogenesis and tumor growth. J Exp Med. 197:221–232. 2003. View Article : Google Scholar : PubMed/NCBI

23 

Ghosh S and Maity P: Augmented antitumor effects of combination therapy with VEGF antibody and cisplatin on murine B16F10 melanoma cells. Int Immunopharmacol. 7:1598–1608. 2007. View Article : Google Scholar : PubMed/NCBI

24 

Blume C, Benz PM, Walter U, et al: AMP-activated protein kinase impairs endothelial actin cytoskeleton assembly by phosphorylating vasodilator-stimulated phosphoprotein. J Biol Chem. 282:4601–4612. 2007. View Article : Google Scholar

25 

Colin D, Gimazane A, Lizard G, et al: Effects of resveratrol analogs on cell cycle progression, cell cycle associated proteins and 5fluoro-uracil sensitivity in human derived colon cancer cells. Int J Cancer. 127:2780–2788. 2009. View Article : Google Scholar

26 

Zhang W, Fei Z, Zhen HN, Zhang JN and Zhang X: Resveratrol inhibits cell growth and induces apoptosis of rat C6 glioma cells. J Neurooncol. 81:231–240. 2007. View Article : Google Scholar

27 

Wu H, Liang X, Fang Y, et al: Resveratrol inhibit hypoxia-induced metastasis potential enhance ment by restricting hypoxia-induced factor-1 alpha expression in colon carcinoma cells. Biomed Pharmacother. 62:613–621. 2008. View Article : Google Scholar : PubMed/NCBI

28 

Chen JC, Chen Y, Lin JH, Wu JM and Tseng SH: Resveratrol suppresses angiogenesis in gliomas: evaluation by color Doppler ultrasound. Anticancer Res. 26:1237–1245. 2006.PubMed/NCBI

29 

Frampton GA, Lazcano EA, Li H, Mohamad A and DeMorrow S: Resveratrol enhances the sensitivity of cholangiocarcinoma to chemotherapeutic agents. Lab Invest. 90:1325–1338. 2010. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Lee SH, Koo BS, Park SY and Kim YM: Anti‑angiogenic effects of resveratrol in combination with 5‑fluorouracil on B16 murine melanoma cells. Mol Med Rep 12: 2777-2783, 2015.
APA
Lee, S.H., Koo, B.S., Park, S.Y., & Kim, Y.M. (2015). Anti‑angiogenic effects of resveratrol in combination with 5‑fluorouracil on B16 murine melanoma cells. Molecular Medicine Reports, 12, 2777-2783. https://doi.org/10.3892/mmr.2015.3675
MLA
Lee, S. H., Koo, B. S., Park, S. Y., Kim, Y. M."Anti‑angiogenic effects of resveratrol in combination with 5‑fluorouracil on B16 murine melanoma cells". Molecular Medicine Reports 12.2 (2015): 2777-2783.
Chicago
Lee, S. H., Koo, B. S., Park, S. Y., Kim, Y. M."Anti‑angiogenic effects of resveratrol in combination with 5‑fluorouracil on B16 murine melanoma cells". Molecular Medicine Reports 12, no. 2 (2015): 2777-2783. https://doi.org/10.3892/mmr.2015.3675
Copy and paste a formatted citation
x
Spandidos Publications style
Lee SH, Koo BS, Park SY and Kim YM: Anti‑angiogenic effects of resveratrol in combination with 5‑fluorouracil on B16 murine melanoma cells. Mol Med Rep 12: 2777-2783, 2015.
APA
Lee, S.H., Koo, B.S., Park, S.Y., & Kim, Y.M. (2015). Anti‑angiogenic effects of resveratrol in combination with 5‑fluorouracil on B16 murine melanoma cells. Molecular Medicine Reports, 12, 2777-2783. https://doi.org/10.3892/mmr.2015.3675
MLA
Lee, S. H., Koo, B. S., Park, S. Y., Kim, Y. M."Anti‑angiogenic effects of resveratrol in combination with 5‑fluorouracil on B16 murine melanoma cells". Molecular Medicine Reports 12.2 (2015): 2777-2783.
Chicago
Lee, S. H., Koo, B. S., Park, S. Y., Kim, Y. M."Anti‑angiogenic effects of resveratrol in combination with 5‑fluorouracil on B16 murine melanoma cells". Molecular Medicine Reports 12, no. 2 (2015): 2777-2783. https://doi.org/10.3892/mmr.2015.3675
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