Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
August-2015 Volume 12 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
August-2015 Volume 12 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Inflammatory cytokine expression following the use of bipolar electrocoagulation, ultracision harmonic scalpel and cold knife biopsy

  • Authors:
    • Pietro Litta
    • Carlo Saccardi
    • Salvatore Gizzo
    • Lorena Conte
    • Giulia Ambrosi
    • Claudia Sissi
    • Manlio Palumbo
  • View Affiliations / Copyright

    Affiliations: Department of Women's and Children's Health, Obstetrics and Gynecology Clinic, University of Padua, Padua 35128, Italy, Department of Pharmaceutical and Pharmacological Sciences, University of Padua, Padua 35128, Italy
  • Pages: 2985-2990
    |
    Published online on: April 24, 2015
       https://doi.org/10.3892/mmr.2015.3677
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Electrical surgical devices may determine tissue damage through lateral thermal spread and activation of inflammatory processes. Several tissue effects are associated with the use of different surgical instruments. The aim of the present study was to compare tissue damage following the application of cold knife biopsy, bipolar electrocoagulation and the ultracision harmonic scalpel, through the analysis of inflammatory gene mediator expression. Three fragments of the round ligament (length 0.5 cm) were obtained from 22 females who had undergone total or subtotal laparoscopic hysterectomy using three different modes of resection: Cold knife biopsy, bipolar electrocoagulation and ultracision harmonic scalpel. The tissue fragments were examined by quantitative polymerase chain reaction (qPCR) analysis of selected cytokines. Gene expression analysis demonstrated large standard deviations due to individual variability among patients and indicated variability in the concentrations of cytokines in the three different samples. The quantity of cytokine mRNA in the cold knife biopsy samples was generally greater than those obtained by other techniques. Tumor necrosis factor‑α expression was significantly higher in the sample obtained with the ultracision harmonic scalpel and bipolar electrocoagulation (P=0.033) when compared with cold knife biopsy. The inflammatory response was analyzed by the quantification of gene expression through the use of qPCR. The ultracision harmonic scalpel and bipolar electrocoagulation triggered the inflammatory cascade and resulted in an increased production of cytokines compared with cold knife biopsy.
View Figures

Figure 1

Figure 2

View References

1 

Harrell AG, Kercher KW and Heniford BT: Energy sources in laparoscopy. Semin Laparosc Surg. 11:201–209. 2004.PubMed/NCBI

2 

Advincula AP and Wang K: The evolutionary state of electrosurgery: where are we now? Curr Opin Obstet Gynecol. 20:353–358. 2008. View Article : Google Scholar : PubMed/NCBI

3 

Gizzo S, Burul G, Di Gangi S, Lamparelli L, Saccardi C, Nardelli GB and D’Antona D: LigaSure vessel sealing system in vaginal hysterectomy: safety, efficacy and limitations. Arch Gynecol Obstet. 288:1067–1074. 2013. View Article : Google Scholar : PubMed/NCBI

4 

Sutton PA, Award S, Perkins AC and Lobo DN: Comparison of lateral thermal spread using monopolar and bipolar diathermy, the Harmonic Scalpel and the Ligasure. Br J Surg. 97:428–433. 2010. View Article : Google Scholar : PubMed/NCBI

5 

Kunde D and Welch C: Ultracision in gynaecological laparoscopic surgery. J Obstet Gynaecol. 23:347–352. 2003. View Article : Google Scholar : PubMed/NCBI

6 

Wallwiener CW, Rajab T, Krämer B, Isaacson KB, Brucker S and Wallwiener M: Quantifying electrosurgery induced thermal effects and damage to human tissue: an exploratory study with the fallopian tube as a novel in-vivo in-situ model. J Minim Invasive Gynecol. 17:70–77. 2010. View Article : Google Scholar

7 

Campbell PA, Cresswell AB, Frank G and Cuschieri A: Real-time thermography during energized vessel sealing and dissection. Surg Endosc. 17:1640–1645. 2003. View Article : Google Scholar : PubMed/NCBI

8 

Jones CJ, Nardo LG, Litta P and Fazleabas AT: Ultrastructure of ectopic peritoneal lesions from women with endometriosis, including observations on the contribution of coelomic mesothelium. Reprod Sci. 16:43–55. 2009. View Article : Google Scholar :

9 

Saccardi C, Gizzo S, Ludwig K, Guido M, Scarton M, Gangemi M, et al: Endometrial polyps in women affected by levothyroxine-treated hypothyroidism – histological features, immunohistochemical findings and possible explanation of etiopathogenic mechanism: a pilot study. Biomed Res Int. 2013:5034192013. View Article : Google Scholar

10 

Gizzo S, Di Gangi S, Bertocco A, Noventa M, Fagherazzi S, Ancona E, et al: Levonorgestrel intrauterine system in adjuvant tamoxifen treatment: balance of breast risks and endometrial benefits – systematic review of literature. Reprod Sci. 21:423–431. 2014. View Article : Google Scholar

11 

Franchi L, Patrelli TS, Berretta R, Rolla M, Gizzo S, Gramellini D, et al: Role of D-dimer testing in severe pelvic inflammatory disease: a new usable marker to assess the need for fertility-impairing surgery? Fertil Steril. 94:2372–2375. 2010. View Article : Google Scholar : PubMed/NCBI

12 

Litta P, Saccardi C, Conte L and Florio P: Reverse hysterectomy: another technique for performing a laparoscopic hysterectomy. J Minim Invasive Gynecol. 20:631–636. 2013. View Article : Google Scholar : PubMed/NCBI

13 

Rasmussen R: Quantification on the LightCycler. Rapid Cycle Real-time PCR, Methods and Applications. Meuer S, Wittwer C and Nakagawara K: Springer Press; Heidelberg: pp. 21–34. 2001

14 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar

15 

Matthews B, Nalysnyk L, Estok R, Fahrbach K, Banel D, Linz H, et al: Ultrasonic and nonultrasonic instrumentation: a systematic review and meta-analysis. Arch Surg. 143:592–600. 2008. View Article : Google Scholar : PubMed/NCBI

16 

Litta P, Fantinato S, Calonaci F, Cosmi E, Filippeschi M, Zerbetto I, et al: A randomized controlled study comparing harmonic versus electrosurgery in laparoscopic myomectomy. Fertil Steril. 94:1882–1886. 2010. View Article : Google Scholar

17 

Holub Z, Jabor A, Kliment L, Lukac J and Voracek J: Laparoscopic lymph node dissection using ultrasonically activated shears: comparison with electrosurgery. J Laparoendosc Adv Surg Tech. 12:175–180. 2002. View Article : Google Scholar

18 

Noventa M, Ancona E, Saccardi C, Litta P, D’Antona D, Nardelli GB, et al: Could HPV-DNA test solve the dilemma about sentinel node frozen section accuracy in early stage cervical cancer? Hypothesis and rationale. Cancer Invest. 32:206–207. 2014. View Article : Google Scholar : PubMed/NCBI

19 

Berretta R, Patrelli TS, Migliavacca C, Rolla M, Franchi L, Monica M, et al: Assessment of tumor size as a useful marker for the surgical staging of endometrial cancer. Oncol Rep. 31:2407–2412. 2014.PubMed/NCBI

20 

Phillips CK, Hruby GW, Durak E, Lehman DS, Humphrey PA, Mansukhani MM, et al: Tissue response to surgical energy devices. Urology. 71:744–748. 2008. View Article : Google Scholar : PubMed/NCBI

21 

Perko Z, Pogorelić Z, Bilan K, Tomić S, Vilović K, Krnić D, et al: Lateral thermal damage to rat abdominal wall after harmonic scalpel application. Surg Endosc. 20:322–324. 2006. View Article : Google Scholar

22 

Holub Z, Jabor A, Sprongl L, Kliment L, Fischlová D and Urbánek S: Inflammatory response and tissue trauma in laparoscopic hysterectomy: comparison of electrosurgery and harmonic scalpel. Clin Exp Obstet Gynecol. 29:105–109. 2002.PubMed/NCBI

23 

Jacobi CA, Wenger F, Opitz I and Müller JM: Immunologic changes during minimally invasive surgery. Dig Surg. 19:459–463. 2002. View Article : Google Scholar : PubMed/NCBI

24 

Härkki-Sirén P, Sjöberg J, Toivonen J and Tiitinen A: Clinical outcome and tissue trauma after laparoscopic and abdominal hysterectomy: a randomized controlled study. Acta Obstet Gynecol Scand. 79:866–871. 2000.

25 

Sietses C, Beelen RH, Meijer S and Cuesta MA: Immunological consequences of laparoscopic surgery, speculations on the cause and clinical implications. Langenbecks Arch Surg. 384:250–258. 1999. View Article : Google Scholar : PubMed/NCBI

26 

Kalff JC, Türler A, Schwarz NT, Schraut WH, Lee KK, Tweardy DJ, et al: Intra-abdominal activation of local infiammatory response within the human muscolaris externa during laparotomy. Ann Surg. 237:301–315. 2003. View Article : Google Scholar : PubMed/NCBI

27 

Sietses C, Eijsbouts QA, von Blomberg BM and Cuesta MA: Ultrasonic energy vs monopolar electrosurgery in laparoscopic cholecystectomy: influence on the postoperative systemic immune response. Surg Endosc. 15:69–71. 2001. View Article : Google Scholar : PubMed/NCBI

28 

Lin E, Calvano SE and Lowry SF: Inflammatory cytokines and cell response in surgery. Surgery. 127:117–126. 2000. View Article : Google Scholar : PubMed/NCBI

29 

Strieter RM, Kunkel SL and Bone RC: Role of tumor necrosis factor-alpha in disease states and inflammation. Crit Care Med. 21:S447–S463. 1993. View Article : Google Scholar : PubMed/NCBI

30 

Amiot F, Fitting C, Tracey KJ, Cavaillon JM and Dautry F: Lipopolysaccharide-induced cytokine cascade and lethality in LT alpha/TNF alpha-deficient mice. Mol Med. 3:864–875. 1997.

31 

Litta P, D’Agostino G, Conte L, Saccardi C, Cela V, Angioni S, et al: Anti-Müllerian hormone trend after laparoscopic surgery in women with ovarian endometrioma. Gynecol Endocrinol. 29:452–454. 2013. View Article : Google Scholar : PubMed/NCBI

32 

Gizzo S, Ancona E, Saccardi C, D’Antona D, Nardelli GB and Plebani M: Could kidney glomerular filtration impairment represent the ‘Achilles heel’ of HE4 serum marker? A possible further implication. Clin Chem Lab Med. 52:e45–e46. 2014. View Article : Google Scholar

33 

Patrelli TS, Berretta R, Gizzo S, Pezzuto A, Franchi L, Lukanovic A, Nardelli GB and Modena AB: CA 125 serum values in surgically treated endometriosis patients and its relationships with anatomic sites of endometriosis and pregnancy rate. Fertil Steril. 95:393–396. 2011. View Article : Google Scholar

34 

Sugita A, Iwase A, Goto M, Nakahara T, Nakamura T, Kondo M, et al: One-year follow-up of serum antimüllerian hormone levels in patients with cystectomy: are different sequential changes due to different mechanisms causing damage to the ovarian reserve? Fertil Steril. 100:516–522. 2013. View Article : Google Scholar

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Litta P, Saccardi C, Gizzo S, Conte L, Ambrosi G, Sissi C and Palumbo M: Inflammatory cytokine expression following the use of bipolar electrocoagulation, ultracision harmonic scalpel and cold knife biopsy. Mol Med Rep 12: 2985-2990, 2015.
APA
Litta, P., Saccardi, C., Gizzo, S., Conte, L., Ambrosi, G., Sissi, C., & Palumbo, M. (2015). Inflammatory cytokine expression following the use of bipolar electrocoagulation, ultracision harmonic scalpel and cold knife biopsy. Molecular Medicine Reports, 12, 2985-2990. https://doi.org/10.3892/mmr.2015.3677
MLA
Litta, P., Saccardi, C., Gizzo, S., Conte, L., Ambrosi, G., Sissi, C., Palumbo, M."Inflammatory cytokine expression following the use of bipolar electrocoagulation, ultracision harmonic scalpel and cold knife biopsy". Molecular Medicine Reports 12.2 (2015): 2985-2990.
Chicago
Litta, P., Saccardi, C., Gizzo, S., Conte, L., Ambrosi, G., Sissi, C., Palumbo, M."Inflammatory cytokine expression following the use of bipolar electrocoagulation, ultracision harmonic scalpel and cold knife biopsy". Molecular Medicine Reports 12, no. 2 (2015): 2985-2990. https://doi.org/10.3892/mmr.2015.3677
Copy and paste a formatted citation
x
Spandidos Publications style
Litta P, Saccardi C, Gizzo S, Conte L, Ambrosi G, Sissi C and Palumbo M: Inflammatory cytokine expression following the use of bipolar electrocoagulation, ultracision harmonic scalpel and cold knife biopsy. Mol Med Rep 12: 2985-2990, 2015.
APA
Litta, P., Saccardi, C., Gizzo, S., Conte, L., Ambrosi, G., Sissi, C., & Palumbo, M. (2015). Inflammatory cytokine expression following the use of bipolar electrocoagulation, ultracision harmonic scalpel and cold knife biopsy. Molecular Medicine Reports, 12, 2985-2990. https://doi.org/10.3892/mmr.2015.3677
MLA
Litta, P., Saccardi, C., Gizzo, S., Conte, L., Ambrosi, G., Sissi, C., Palumbo, M."Inflammatory cytokine expression following the use of bipolar electrocoagulation, ultracision harmonic scalpel and cold knife biopsy". Molecular Medicine Reports 12.2 (2015): 2985-2990.
Chicago
Litta, P., Saccardi, C., Gizzo, S., Conte, L., Ambrosi, G., Sissi, C., Palumbo, M."Inflammatory cytokine expression following the use of bipolar electrocoagulation, ultracision harmonic scalpel and cold knife biopsy". Molecular Medicine Reports 12, no. 2 (2015): 2985-2990. https://doi.org/10.3892/mmr.2015.3677
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team