Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
August-2015 Volume 12 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
August-2015 Volume 12 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

MicroRNA-218, microRNA-191*, microRNA-3070a and microRNA-33 are responsive to mechanical strain exerted on osteoblastic cells

  • Authors:
    • Yong Guo
    • Yang Wang
    • Yinqin Liu
    • Yongming Liu
    • Qiangcheng Zeng
    • Yumin Zhao
    • Xinchang Zhang
    • Xizheng Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Biomedical Engineering, College of Biotechnology, Guilin Medical University, Guilin, Guangxi 541004, P.R. China, Shandong Provincial Key Laboratory of Functional Macromolecular Biophysics, Institute of Biophysics, Dezhou University, Dezhou, Shandong 253000, P.R. China, Lab of Biomechanics, Institute of Medical Equipment, Academy of Military Medical Sciences, Tianjin 300161, P.R. China
  • Pages: 3033-3038
    |
    Published online on: April 30, 2015
       https://doi.org/10.3892/mmr.2015.3705
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

MicroRNA (miRNA) is an important regulator of cell differentiation and function. Mechanical strain is important in the growth and differentiation of osteoblasts. Therefore, mechanresponsive miRNA may be important in the response of osteoblasts to mechanical strain. The purpose of the present study was to select and identify the mechanoresponsive miRNAs of osteoblasts. Mouse osteoblastic MC3T3‑E1 cells were cultured in cell culture dishes and stimulated with a mechanical tensile strain of 2,50 µε at 0.5 Hz, and the activity of alkaline phosphatase (ALP), mRNA levels of ALP, osteocalcin (OCN), and collagen type I (Col I), and protein levels of bone morphogenetic proteins (BMPs) inthe cell culture medium were assayed. Following miRNA microarray and reverse transcription‑quantitative polymerase chain reaction analyses, differentially expressed miRNAs in the mechanically strained cells and unstrained cells were selected and identified. Using bioinformatics analysis, the target genes of the miRNAs were then predicted. The results revealed that the mechanical strain of 2,500 µε increased the activity of ALP, the mRNA levels of ALP, OCN and Col I, and the protein levels of bone morphogenetic protein(BMP)‑2 and BMP‑4 Continuous mechanical stimulation for 8 h had the most marked stimulant effects. miR‑218, miR‑191*, miR‑3070a and miR‑33 were identified as differentially expressed miRNAs in the mechanically strained MC3T3‑E1 cells. Certain target genes of these four miRNAs were involved in osteoblastic differentiation. These findings indicated that a mechanical strain of 2,500 µε, particularly for a period of 8 h, promoted osteoblastic differentiation, and the four mechanoresponsive miRNAs identified may be a potential regulator of osteoblastic differentiation and their response to mechanical strain.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

View References

1 

Schriefer JL, Warden SJ, Saxon LK, et al: Cellular accommodation and the response of bone to mechanical loading. J Biomech. 38:1838–1845. 2005. View Article : Google Scholar : PubMed/NCBI

2 

Thompson WR, Rubin CT and Rubin J: Mechanical regulation of signaling pathways in bone. Gene. 503:179–193. 2012. View Article : Google Scholar : PubMed/NCBI

3 

Wozniak M, Fausto A, Carron CP, Meyer DM and Hruska KA: Mechanically strained cells of the osteoblast lineageorganize their extracellular matrix through unique sites of alphavbeta3-integrin expression. J Bone Miner Res. 15:1731–1745. 2000. View Article : Google Scholar : PubMed/NCBI

4 

Kaneuji T, Nogami S, Ariyoshi W, et al: Regulatory effect on osteoclastogenesis of mechanical strain-loaded osteoblasts. Int J Oral Maxillofac Surg. 40:12152011. View Article : Google Scholar

5 

Rumney RM, Sunters A, Reilly GC and Gartland A: Application of multiple forms of mechanical loading to human osteoblasts reveals increased ATP release in response to fluid flow in 3D cultures and differential regulation of immediate early genes. J Biomech. 45:549–554. 2012. View Article : Google Scholar :

6 

Zhao Y and Srivastava D: A developmental view of microRNA function. Trends Biochem Sci. 32:189–197. 2007. View Article : Google Scholar : PubMed/NCBI

7 

Bartel DP: MicroRNAs: Genomics, biogenesis, mechanism and function. Cell. 116:281–297. 2004. View Article : Google Scholar : PubMed/NCBI

8 

Stefani G and Slack FJ: Small non-coding RNAs in animal development. Nat Rev Mol Cell Biol. 9:219–230. 2008. View Article : Google Scholar : PubMed/NCBI

9 

Taipaleenmäki H, Bjerre Hokland L, Chen L, Kauppinen S and Kassem M: Mechanisms in endocrinology: micro-RNAs: targets for enhancing osteoblast differentiation and bone formation. Eur J Endocrinol. 166:359–371. 2012. View Article : Google Scholar

10 

Vimalraj S and Selvamurugan N: MicroRNAs: synthesis, gene regulation and osteoblast differentiation. Curr Issues Mol Biol. 15:7–18. 2012.PubMed/NCBI

11 

Ni CW, Qiu H and Jo H: MicroRNA-663 upregulated by oscillatory shear stress plays a role in inflammatory response of endothelial cells. Am J Physiol Heart Circ Physiol. 300:H1762–H1769. 2011. View Article : Google Scholar : PubMed/NCBI

12 

Guan YJ, Yang X, Wei L and Chen Q: MiR-365: a mechanosen-sitive microRNA stimulates chondrocyte differentiation through targeting histone deacetylase 4. FASEB J. 25:4457–4466. 2011. View Article : Google Scholar : PubMed/NCBI

13 

Song JT, Hu B, Qu HY, et al: Mechanical stretch modulates microRNA-21 expression, participating in proliferation and apoptosis in cultured human aortic smooth muscle cells. PLoS One. 7:e476572012. View Article : Google Scholar

14 

Tang LL, Wang YL, Pan J and Cai SX: The effect of step-wise increased stretching on rat calvarial osteoblast collagen production. J Biomech. 37:157–161. 2004. View Article : Google Scholar

15 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real time quantitative PCR and the 2(−Delta Delta C(T)) Method. Methods. 25:402–408. 2001. View Article : Google Scholar

16 

Jang WG, Kim EJ and Koh JT: Tunicamycin negatively regulates BMP2-induced osteoblast differentiation through CREBH expression in MC3T3E1 cells. BMB Rep. 44:735–740. 2011. View Article : Google Scholar : PubMed/NCBI

17 

Wan M and Cao X: BMP signaling in skeletal development. Biochen Biophys Res Commun. 328:651–657. 2005. View Article : Google Scholar

18 

Guo Y, Zhang CQ, Zeng QC, et al: Mechanical strain promotes osteoblast ECM formation and improves its osteoinductive potential. Biomed Eng Online. 11:802012. View Article : Google Scholar : PubMed/NCBI

19 

Rubin CT and Lanyon LE: Regulation of bone formation by applied dynamic loads. J Bone Joint Surg Am. 66:397–402. 1984.PubMed/NCBI

20 

Lanyon LE and Rubin CT: Static versus dynamic loads as an influence on bone remodelling. J Biomech. 17:897–905. 1984. View Article : Google Scholar

21 

Hillam RA and Skerry TM: Inhibition of bone resorption and stimulation offormulation by mechanical loading of the modeling rat ulna in vivo. J Bone Miner Res. 10:683–689. 1995. View Article : Google Scholar : PubMed/NCBI

22 

Turner CH and Pavalko FM: Mechanotransduction and functional responseof the skeleton to physical stress: the mechanisms and mechanics of bone adaptation. J Orthop Sci. 3:346–355. 1998. View Article : Google Scholar

23 

Wozniak M, Fausto A, Carron CP, Meyer DM and Hruska KA: Mechanically strained cells of the osteoblast lineage organize their extracellular matrix through unique sites of alphavbeta3-integrin expression. J Bone Miner Res. 15:1731–1745. 2000. View Article : Google Scholar : PubMed/NCBI

24 

Bhatt KA, Chang EI, Warren SM, et al: Uniaxial mechanical strain: an in vitro correlate to distraction osteogenesis. J Surg Res. 143:329–336. 2007. View Article : Google Scholar : PubMed/NCBI

25 

Ducy P, Zhang R, Geoffroy V, Ridall AL and Karsenty G: Osf2/Cbfa1: a transcriptional activator of osteoblast differentiation. Cell. 89:747–754. 1997. View Article : Google Scholar : PubMed/NCBI

26 

Olivares-Navarrete R, Hyzy S, Wieland M, Boyan BD and Schwartz Z: The roles of Wnt signaling modulators Dickkopf-1 (Dkk1) and Dickkopf-2 (Dkk2) and cell maturation state in osteogenesis on microstructured titanium surfaces. Biomaterials. 31:2015–2024. 2010. View Article : Google Scholar

27 

Lin TH, Yang RS, Tang CH, Wu MY and Fu WM: Regulation of the maturation of osteoblasts and osteoclastogenesis by glutamate. Eur J Pharmacol. 589:37–44. 2008. View Article : Google Scholar : PubMed/NCBI

28 

Szczesniak AM, Gilbert RW, Mukhida M and Anderson GI: Mechanical loading modulates glutamate receptor subunit expression in bone. Bone. 37:63–73. 2005. View Article : Google Scholar : PubMed/NCBI

29 

Danciu TE, Adam RM, Naruse K, Freeman MR and Hauschka PV: Calcium regulates the PI3K-Akt pathway in stretched osteoblasts. FEBS Lett. 536:193–197. 2003. View Article : Google Scholar : PubMed/NCBI

30 

Futatsugi A, Nakamura T, Yamada MK, et al: IP3 receptor types 2 and 3 mediate exocrine secretion underlying energy metabolism. Science. 309:2232–2234. 2005. View Article : Google Scholar : PubMed/NCBI

31 

Lin GL and Hankenson KD: Integration of BMP, Wnt and notch signaling pathways in osteoblast differentiation. J Cell Biochem. 112:3491–501. 2011. View Article : Google Scholar : PubMed/NCBI

32 

Ito Y, Inoue D, Kido S and Matsumoto T: c-Fos degradation by the ubiquitin-proteasome proteolytic pathway in osteoclast progenitors. Bone. 37:842–849. 2005. View Article : Google Scholar : PubMed/NCBI

33 

Xing L, Zhang M and Chen D: Smurf control in bone cells. J Cell Biochem. 110:554–563. 2010. View Article : Google Scholar : PubMed/NCBI

34 

Hatakeyama N, Kojima T, Iba K, et al: IGF-I regulates tight-junction protein claudin-1 during differentiation of osteoblast-like MC3T3-E1 cells via a MAP-kinase pathway. Cell Tissue Res. 334:243–254. 2008. View Article : Google Scholar : PubMed/NCBI

35 

Greenblatt MB, Shim JH, Zou W, et al: The p38 MAPK pathway is essential for skeletogenesis and bone homeostasis in mice. J Clin Invest. 120:2457–2473. 2010. View Article : Google Scholar : PubMed/NCBI

36 

Nakano Y, Forsprecher J and Kaartinen MT: Regulation of ATPase activity of transglutaminase 2 by MT1-MMP: implications for mineralization of MC3T3-E1 osteoblast cultures. J Cell Physiol. 223:260–269. 2010.PubMed/NCBI

37 

Sun D, Junger WG, Yuan C, et al: Shockwaves induce osteogenic differentiation of human mesenchymal stem cells through ATP release and activation of P2×7 receptors. Stem Cells. 31:1170–80. 2013. View Article : Google Scholar : PubMed/NCBI

38 

Chen G, Deng C and Li YP: TGF-β and BMP signaling in osteoblast differentiation and bone formation. Int J Biol Sci. 8:272–288. 2012. View Article : Google Scholar

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Guo Y, Wang Y, Liu Y, Liu Y, Zeng Q, Zhao Y, Zhang X and Zhang X: MicroRNA-218, microRNA-191*, microRNA-3070a and microRNA-33 are responsive to mechanical strain exerted on osteoblastic cells. Mol Med Rep 12: 3033-3038, 2015.
APA
Guo, Y., Wang, Y., Liu, Y., Liu, Y., Zeng, Q., Zhao, Y. ... Zhang, X. (2015). MicroRNA-218, microRNA-191*, microRNA-3070a and microRNA-33 are responsive to mechanical strain exerted on osteoblastic cells. Molecular Medicine Reports, 12, 3033-3038. https://doi.org/10.3892/mmr.2015.3705
MLA
Guo, Y., Wang, Y., Liu, Y., Liu, Y., Zeng, Q., Zhao, Y., Zhang, X., Zhang, X."MicroRNA-218, microRNA-191*, microRNA-3070a and microRNA-33 are responsive to mechanical strain exerted on osteoblastic cells". Molecular Medicine Reports 12.2 (2015): 3033-3038.
Chicago
Guo, Y., Wang, Y., Liu, Y., Liu, Y., Zeng, Q., Zhao, Y., Zhang, X., Zhang, X."MicroRNA-218, microRNA-191*, microRNA-3070a and microRNA-33 are responsive to mechanical strain exerted on osteoblastic cells". Molecular Medicine Reports 12, no. 2 (2015): 3033-3038. https://doi.org/10.3892/mmr.2015.3705
Copy and paste a formatted citation
x
Spandidos Publications style
Guo Y, Wang Y, Liu Y, Liu Y, Zeng Q, Zhao Y, Zhang X and Zhang X: MicroRNA-218, microRNA-191*, microRNA-3070a and microRNA-33 are responsive to mechanical strain exerted on osteoblastic cells. Mol Med Rep 12: 3033-3038, 2015.
APA
Guo, Y., Wang, Y., Liu, Y., Liu, Y., Zeng, Q., Zhao, Y. ... Zhang, X. (2015). MicroRNA-218, microRNA-191*, microRNA-3070a and microRNA-33 are responsive to mechanical strain exerted on osteoblastic cells. Molecular Medicine Reports, 12, 3033-3038. https://doi.org/10.3892/mmr.2015.3705
MLA
Guo, Y., Wang, Y., Liu, Y., Liu, Y., Zeng, Q., Zhao, Y., Zhang, X., Zhang, X."MicroRNA-218, microRNA-191*, microRNA-3070a and microRNA-33 are responsive to mechanical strain exerted on osteoblastic cells". Molecular Medicine Reports 12.2 (2015): 3033-3038.
Chicago
Guo, Y., Wang, Y., Liu, Y., Liu, Y., Zeng, Q., Zhao, Y., Zhang, X., Zhang, X."MicroRNA-218, microRNA-191*, microRNA-3070a and microRNA-33 are responsive to mechanical strain exerted on osteoblastic cells". Molecular Medicine Reports 12, no. 2 (2015): 3033-3038. https://doi.org/10.3892/mmr.2015.3705
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team