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Article Open Access

Impact of immunosuppressive agents on the expression of indoleamine 2,3-dioxygenase, heme oxygenase-1 and interleukin-7 in mesangial cells

  • Authors:
    • Guo‑Biao Liang
    • Guang‑Heng Luo
    • Ding‑Su Bao
    • An‑Jian Chen
    • Yong‑Xiang Zhuang
    • Ya‑Nan Guo
    • Xin Wang
    • Yuan‑Liang Wang
    • Zong‑Ping Chen
    • Yi‑Ping Lu
    • You‑Ping Li
  • View Affiliations / Copyright

    Affiliations: Department of Urology, Affiliated Hospital of Zunyi Medical College, Zunyi, Guizhou 563003, P.R. China, Department of Urology, Guizhou Provincial People's Hospital, Guiyang, Guizhou 550005, P.R. China, Department of Urology, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China, Transplantation Immunology Laboratory, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China
    Copyright: © Liang et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 2577-2583
    |
    Published online on: May 4, 2015
       https://doi.org/10.3892/mmr.2015.3713
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Abstract

Chronic allograft nephropathy (CAN) is a major cause of graft loss following kidney transplantation and may result from the interactions of various immune and non‑immune factors. The aim of the present study was to establish an in vitro model of glomerular mesangial cell injury in order to examine the gene expression levels of indoleamine 2,3‑dioxygenase (IDO), heme oxygenase‑1 (HO‑1) and interleukin‑7 (IL‑7) in mesangial cells during the healing process as well as to investigate the effects of various immunosuppressants on the expression of these genes. The HBZY‑1 glomerular mesangial cell line was pre‑treated in vitro with cytochalasin B for 2 h to induce reversible damage. Following the pre‑treatment, the HBZY‑1 cells were divided into five groups: Blank control group, cyclosporine A (CsA) group, tacrolimus (Tac) group, mycophenolate mofetil (MMF) group and rapamycin (RAPA) group. After treating the mesangial cells with each immunosuppressive drug for 6, 12 or 24 h, the mRNA and protein expression levels of IDO, HO‑1 and IL‑7 were examined using reverse transcription quantitative polymerase chain reaction (RT‑qPCR), western blot and immunohistochemical analyses. The results showed that expression levels of HO‑1 were significantly upregulated in response to treatment with CsA, FK506, RAPA and MMF, whereas the expression levels of IL‑7 were markedly downregulated by treatment with the above immunosuppressants. CsA, FK506 and MMF significantly enhanced the expression levels of IDO, whereas RAPA exhibited no apparent effect on IDO. The present study may contribute to the understanding of the pathogenesis of CAN and provide novel strategies for the prevention and treatment of CAN.
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Copy and paste a formatted citation
Spandidos Publications style
Liang GB, Luo GH, Bao DS, Chen AJ, Zhuang YX, Guo YN, Wang X, Wang YL, Chen ZP, Lu YP, Lu YP, et al: Impact of immunosuppressive agents on the expression of indoleamine 2,3-dioxygenase, heme oxygenase-1 and interleukin-7 in mesangial cells. Mol Med Rep 12: 2577-2583, 2015.
APA
Liang, G., Luo, G., Bao, D., Chen, A., Zhuang, Y., Guo, Y. ... Li, Y. (2015). Impact of immunosuppressive agents on the expression of indoleamine 2,3-dioxygenase, heme oxygenase-1 and interleukin-7 in mesangial cells. Molecular Medicine Reports, 12, 2577-2583. https://doi.org/10.3892/mmr.2015.3713
MLA
Liang, G., Luo, G., Bao, D., Chen, A., Zhuang, Y., Guo, Y., Wang, X., Wang, Y., Chen, Z., Lu, Y., Li, Y."Impact of immunosuppressive agents on the expression of indoleamine 2,3-dioxygenase, heme oxygenase-1 and interleukin-7 in mesangial cells". Molecular Medicine Reports 12.2 (2015): 2577-2583.
Chicago
Liang, G., Luo, G., Bao, D., Chen, A., Zhuang, Y., Guo, Y., Wang, X., Wang, Y., Chen, Z., Lu, Y., Li, Y."Impact of immunosuppressive agents on the expression of indoleamine 2,3-dioxygenase, heme oxygenase-1 and interleukin-7 in mesangial cells". Molecular Medicine Reports 12, no. 2 (2015): 2577-2583. https://doi.org/10.3892/mmr.2015.3713
Copy and paste a formatted citation
x
Spandidos Publications style
Liang GB, Luo GH, Bao DS, Chen AJ, Zhuang YX, Guo YN, Wang X, Wang YL, Chen ZP, Lu YP, Lu YP, et al: Impact of immunosuppressive agents on the expression of indoleamine 2,3-dioxygenase, heme oxygenase-1 and interleukin-7 in mesangial cells. Mol Med Rep 12: 2577-2583, 2015.
APA
Liang, G., Luo, G., Bao, D., Chen, A., Zhuang, Y., Guo, Y. ... Li, Y. (2015). Impact of immunosuppressive agents on the expression of indoleamine 2,3-dioxygenase, heme oxygenase-1 and interleukin-7 in mesangial cells. Molecular Medicine Reports, 12, 2577-2583. https://doi.org/10.3892/mmr.2015.3713
MLA
Liang, G., Luo, G., Bao, D., Chen, A., Zhuang, Y., Guo, Y., Wang, X., Wang, Y., Chen, Z., Lu, Y., Li, Y."Impact of immunosuppressive agents on the expression of indoleamine 2,3-dioxygenase, heme oxygenase-1 and interleukin-7 in mesangial cells". Molecular Medicine Reports 12.2 (2015): 2577-2583.
Chicago
Liang, G., Luo, G., Bao, D., Chen, A., Zhuang, Y., Guo, Y., Wang, X., Wang, Y., Chen, Z., Lu, Y., Li, Y."Impact of immunosuppressive agents on the expression of indoleamine 2,3-dioxygenase, heme oxygenase-1 and interleukin-7 in mesangial cells". Molecular Medicine Reports 12, no. 2 (2015): 2577-2583. https://doi.org/10.3892/mmr.2015.3713
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