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Article

Role of ROCK expression in gallbladder smooth muscle contraction

  • Authors:
    • Bin Wang
    • You‑Ming Ding
    • Chun‑Tao Wang
    • Wei‑Xing Wang
  • View Affiliations / Copyright

    Affiliations: Department of Hepatobiliary and Laparoscopic Surgery, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China
  • Pages: 2907-2911
    |
    Published online on: May 4, 2015
       https://doi.org/10.3892/mmr.2015.3726
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Abstract

Cholelithiasis is a common medical condition whose incidence rate is increasing yearly, while its pathogenesis has yet to be elucidated. The present study assessed the expression of Rho‑kinase (ROCK) in gallbladder smooth muscles and its effect on the contractile function of gallbladder smooth muscles during gallstone formation. Thirty male guinea pigs were randomly divided into three groups: The control group, the gallstone model group and the fasudil interference group. The fasting volume (FV) and bile capacity of the gallbladder (FB) as well as the total cholesterol (TC) and triglyceride (TG) contents of the gallbladder bile were determined. In addition, the gallbladder was dissected to identify whether any gallstones had formed. Part of the gallbladder tissue specimens were used for immunohistochemical analysis of ROCK expression in gallbladder smooth muscles. The results showed that four guinea pigs in the model group and eight in the fasudil group displayed gallstone formation, while there was no gallstone formation in the control group. The FV and FB were significantly increased in the model and fasudil groups. Similarly, the TC and TG contents of gallbladder bile were increased in these groups. The positive expression rate of ROCK in gallbladder smooth muscles in the model and fasudil groups was significantly reduced compared with that in the control group (P<0.05). The results of the present study indicated that the reduction of ROCK expression in guinea pig gallbladder smooth muscles weakened gallbladder contraction and thereby promoted gallstone formation.
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Copy and paste a formatted citation
Spandidos Publications style
Wang B, Ding YM, Wang CT and Wang WX: Role of ROCK expression in gallbladder smooth muscle contraction. Mol Med Rep 12: 2907-2911, 2015.
APA
Wang, B., Ding, Y., Wang, C., & Wang, W. (2015). Role of ROCK expression in gallbladder smooth muscle contraction. Molecular Medicine Reports, 12, 2907-2911. https://doi.org/10.3892/mmr.2015.3726
MLA
Wang, B., Ding, Y., Wang, C., Wang, W."Role of ROCK expression in gallbladder smooth muscle contraction". Molecular Medicine Reports 12.2 (2015): 2907-2911.
Chicago
Wang, B., Ding, Y., Wang, C., Wang, W."Role of ROCK expression in gallbladder smooth muscle contraction". Molecular Medicine Reports 12, no. 2 (2015): 2907-2911. https://doi.org/10.3892/mmr.2015.3726
Copy and paste a formatted citation
x
Spandidos Publications style
Wang B, Ding YM, Wang CT and Wang WX: Role of ROCK expression in gallbladder smooth muscle contraction. Mol Med Rep 12: 2907-2911, 2015.
APA
Wang, B., Ding, Y., Wang, C., & Wang, W. (2015). Role of ROCK expression in gallbladder smooth muscle contraction. Molecular Medicine Reports, 12, 2907-2911. https://doi.org/10.3892/mmr.2015.3726
MLA
Wang, B., Ding, Y., Wang, C., Wang, W."Role of ROCK expression in gallbladder smooth muscle contraction". Molecular Medicine Reports 12.2 (2015): 2907-2911.
Chicago
Wang, B., Ding, Y., Wang, C., Wang, W."Role of ROCK expression in gallbladder smooth muscle contraction". Molecular Medicine Reports 12, no. 2 (2015): 2907-2911. https://doi.org/10.3892/mmr.2015.3726
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