Open Access

Hydrogen-rich saline reduces cell death through inhibition of DNA oxidative stress and overactivation of poly (ADP-ribose) polymerase-1 in retinal ischemia-reperfusion injury

  • Authors:
    • Hongwei Liu
    • Ning Hua
    • Keliang Xie
    • Tingting Zhao
    • Yonghao Yu
  • View Affiliations

  • Published online on: May 5, 2015     https://doi.org/10.3892/mmr.2015.3731
  • Pages: 2495-2502
  • Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

Overactivation of poly (ADP-ribose) polymerase 1 (PARP-1), as a result of sustained DNA oxidation in ischemia‑reperfusion injury, triggers programmed cell necrosis and apoptosis. The present study was conducted to demonstrate whether hydrogen‑rich saline (HRS) has a neuroprotective effect on retinal ischemia reperfusion (RIR) injury through inhibition of PARP‑1 activation. RIR was induced by transient elevation of intraocular pressure in rats. HRS (5 ml/kg) was administered peritoneally every day from the beginning of reperfusion in RIR rats until the rats were sacrificed. Retinal damage and cell death was determined using hematoxylin and eosin and terminal deoxynucleotidyl transferase dUTP nick end labeling staining. DNA oxidative stress was evaluated by immunofluorescence staining of 8‑hydroxy‑2‑deoxyguanosine. In addition, the expression of PARP‑1 and caspase‑3 was investigated by western blot analysis and/or immunohistochemical staining. The results demonstrated that HRS administration improved morphological alterations and reduced apoptosis following RIR injury. Furthermore, the present study found that HRS alleviated DNA oxidation and PARP‑1 overactivation in RIR rats. HRS can protect RIR injury by inhibition of PARP‑1, which may be involved in DNA oxidative stress and caspase-3-mediated apoptosis.
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August-2015
Volume 12 Issue 2

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Liu H, Hua N, Xie K, Zhao T and Yu Y: Hydrogen-rich saline reduces cell death through inhibition of DNA oxidative stress and overactivation of poly (ADP-ribose) polymerase-1 in retinal ischemia-reperfusion injury. Mol Med Rep 12: 2495-2502, 2015
APA
Liu, H., Hua, N., Xie, K., Zhao, T., & Yu, Y. (2015). Hydrogen-rich saline reduces cell death through inhibition of DNA oxidative stress and overactivation of poly (ADP-ribose) polymerase-1 in retinal ischemia-reperfusion injury. Molecular Medicine Reports, 12, 2495-2502. https://doi.org/10.3892/mmr.2015.3731
MLA
Liu, H., Hua, N., Xie, K., Zhao, T., Yu, Y."Hydrogen-rich saline reduces cell death through inhibition of DNA oxidative stress and overactivation of poly (ADP-ribose) polymerase-1 in retinal ischemia-reperfusion injury". Molecular Medicine Reports 12.2 (2015): 2495-2502.
Chicago
Liu, H., Hua, N., Xie, K., Zhao, T., Yu, Y."Hydrogen-rich saline reduces cell death through inhibition of DNA oxidative stress and overactivation of poly (ADP-ribose) polymerase-1 in retinal ischemia-reperfusion injury". Molecular Medicine Reports 12, no. 2 (2015): 2495-2502. https://doi.org/10.3892/mmr.2015.3731