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Article

Decreased mRNA and protein expression of TWIST1 in myocardial tissue of fetuses with ventricular septal defects

  • Authors:
    • Yuting Wang
    • Qidi Wang
    • Changlong Guo
    • Shuo Wang
    • Yue Qiu
    • Hui Li
    • Xu Ma
  • View Affiliations / Copyright

    Affiliations: Department of Obstetrics and Gynecology, Shengjing Hospital of China Medical University, Shenyang, Liaoning 110004, P.R. China, Department of Genetics, National Research Institute for Family Planning, Beijing 100081, P.R. China
  • Pages: 3089-3094
    |
    Published online on: May 6, 2015
       https://doi.org/10.3892/mmr.2015.3734
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Abstract

Ventricular septal defect (VSD) is the most common type of congenital heart disease (CHD). The single gene mutations or absences that contribute to VSD development are well established; however, the aim of the present study was to measure gene expression variation between VSDs and normal fetal myocardial tissue. TWIST1, an important tumor biomarker, is a basic helix‑loop‑helix transcription factor that regulates cell proliferation, migration and differentiation in embryonic development and transformed tumor cells. Although growing evidence demonstrates that TWIST1 participates in a variety of human neoplastic diseases, the role of TWIST1 in VSD has remained elusive. Twenty‑six VSD fetal myocardial tissue samples and 12 normal samples at matched gestational weeks (22‑28 weeks) were included in the present study. Using reverse transcription quantitative polymerase chain reaction (PCR) and real‑time PCR, it was demonstrated that TWIST1 mRNA was reduced by almost two‑fold in the VSD samples compared with the normal samples. Western blot analysis also revealed that TWIST1 expression was decreased by ~three‑fold (P=0.001) in the VSD samples compared with that in the normal samples. Of note, five complete ventricular (also called functionally univentricular or single ventricular) septal ageneses were identified among the specimens. For the five complete ventricular septal agenesis samples, similar results to those for other VSD fetal myocardial tissues were obtained. In conclusion, the results of the present study showed that TWIST1 mRNA and protein levels were reduced in VSDs. The present study was the first, to the best of our knowledge, to report that TWIST1 is not only a tumor biomarker, but may also be involved in the pathogenesis of VSD.
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Copy and paste a formatted citation
Spandidos Publications style
Wang Y, Wang Q, Guo C, Wang S, Qiu Y, Li H and Ma X: Decreased mRNA and protein expression of TWIST1 in myocardial tissue of fetuses with ventricular septal defects. Mol Med Rep 12: 3089-3094, 2015.
APA
Wang, Y., Wang, Q., Guo, C., Wang, S., Qiu, Y., Li, H., & Ma, X. (2015). Decreased mRNA and protein expression of TWIST1 in myocardial tissue of fetuses with ventricular septal defects. Molecular Medicine Reports, 12, 3089-3094. https://doi.org/10.3892/mmr.2015.3734
MLA
Wang, Y., Wang, Q., Guo, C., Wang, S., Qiu, Y., Li, H., Ma, X."Decreased mRNA and protein expression of TWIST1 in myocardial tissue of fetuses with ventricular septal defects". Molecular Medicine Reports 12.2 (2015): 3089-3094.
Chicago
Wang, Y., Wang, Q., Guo, C., Wang, S., Qiu, Y., Li, H., Ma, X."Decreased mRNA and protein expression of TWIST1 in myocardial tissue of fetuses with ventricular septal defects". Molecular Medicine Reports 12, no. 2 (2015): 3089-3094. https://doi.org/10.3892/mmr.2015.3734
Copy and paste a formatted citation
x
Spandidos Publications style
Wang Y, Wang Q, Guo C, Wang S, Qiu Y, Li H and Ma X: Decreased mRNA and protein expression of TWIST1 in myocardial tissue of fetuses with ventricular septal defects. Mol Med Rep 12: 3089-3094, 2015.
APA
Wang, Y., Wang, Q., Guo, C., Wang, S., Qiu, Y., Li, H., & Ma, X. (2015). Decreased mRNA and protein expression of TWIST1 in myocardial tissue of fetuses with ventricular septal defects. Molecular Medicine Reports, 12, 3089-3094. https://doi.org/10.3892/mmr.2015.3734
MLA
Wang, Y., Wang, Q., Guo, C., Wang, S., Qiu, Y., Li, H., Ma, X."Decreased mRNA and protein expression of TWIST1 in myocardial tissue of fetuses with ventricular septal defects". Molecular Medicine Reports 12.2 (2015): 3089-3094.
Chicago
Wang, Y., Wang, Q., Guo, C., Wang, S., Qiu, Y., Li, H., Ma, X."Decreased mRNA and protein expression of TWIST1 in myocardial tissue of fetuses with ventricular septal defects". Molecular Medicine Reports 12, no. 2 (2015): 3089-3094. https://doi.org/10.3892/mmr.2015.3734
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