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Article

Screening biomarkers of bladder cancer using combined miRNA and mRNA microarray analysis

  • Authors:
    • Ning Jin
    • Xuefei Jin
    • Xinquan Gu
    • Wanli Na
    • Muchun Zhang
    • Rui Zhao
  • View Affiliations / Copyright

    Affiliations: Department of Urology, China‑Japan Union Hospital of Jilin University, Changchun, Jilin 130033, P.R. China
  • Pages: 3170-3176
    |
    Published online on: May 7, 2015
       https://doi.org/10.3892/mmr.2015.3739
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Abstract

Biomarkers, such as microRNAs (miRNAs) may be useful for the diagnosis of bladder cancer. In order to understand the molecular mechanisms underlying bladder cancer, differentially expressed miRNAs (DE‑miRNAs) and their target genes in bladder cancer were analyzed. In the present study, miRNA and mRNA expression profiles (GSE40355) were obtained from the Gene Expression Omnibus. These consisted of healthy bladder samples (n=8) and urothelial carcinoma samples (low‑grade, n=8 and high‑grade, n=8). DE‑miRNAs and differentially expressed genes (DEGs) were identified using the limma package and the Benjamin and Hochberg method from the multtest package in R. Target genes of DE‑miRNAs were screened. Associations between DEGs were investigated using STRING, and an interaction network was constructed using Cytoscape. Functional and pathway enrichment analyses were performed for DEGs from the interaction network. 87 DE‑miRNAs and 2058 DEGs were screened from low‑grade bladder cancer samples, and 40 DE‑miRNAs and 2477 DEGs were screened from high‑grade bladder cancer samples. DE‑target genes were significantly associated with the regulation of cell apoptosis. Bladder cancer, non‑small cell lung cancer and pancreatic cancer biological pathways were found to be enriched. The results of the present study demonstrated that E2F transcription factor 1, which is targeted by miR‑106b, and cyclin‑dependent kinase inhibitor 2A (CDKN2A) and V‑Erb‑B2 avian erythroblastic leukemia viral oncogene homolog‑2, which are targeted by miR‑125b, participate in the bladder cancer pathway. In conclusion, DE‑miRNAs in bladder cancer tissue samples and DE‑targeted genes, such as miR‑106b and CDKN2A, which were identified in the present study, may provide the basis for targeted therapy for breast cancer and enhance understanding of its pathogenesis.
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1 

Jemal A, Siegel R, Xu J and Ward E: Cancer statistics, 2010. CA Cancer J Clin. 60:277–300. 2010. View Article : Google Scholar : PubMed/NCBI

2 

Shariat SF, Casella R, Khoddami SM, Hernandez G, Sulser T, Gasser TC and Lerner SP: Urine detection of survivin is a sensitive marker for the noninvasive diagnosis of bladder cancer. J Urol. 171:626–630. 2004. View Article : Google Scholar : PubMed/NCBI

3 

Parkin DM: The global burden of urinary bladder cancer. Scand J Urol Nephrol Suppl. 218:12–20. 2008. View Article : Google Scholar : PubMed/NCBI

4 

Kadlubar FF and Badawi AF: Genetic susceptibility and carcinogen-DNA adduct formation in human urinary bladder carcinogenesis. Toxicol Lett. 82–83:627–632. 1995. View Article : Google Scholar

5 

Jung I and Messing E: Molecular mechanisms and pathways in bladder cancer development and progression. Cancer Control. 7:325–334. 2000.PubMed/NCBI

6 

Kaderlik KR and Kadlubar FF: Metabolic polymorphisms and carcinogen-DNA adduct formation in human populations. Pharmacogenetics. 5:S108–S117. 1995. View Article : Google Scholar : PubMed/NCBI

7 

Bell DA, Taylor JA, Paulson DF, Robertson CN, Mohler JL and Lucier GW: Genetic risk and carcinogen exposure: A common inherited defect of the carcinogen-metabolism gene glutathione S-transferase M1 (GSTM1) that increases susceptibility to bladder cancer. J Natl Cancer Inst. 85:1159–1164. 1993. View Article : Google Scholar : PubMed/NCBI

8 

Zhang B, Pan X, Cobb GP and Anderson TA: microRNAs as oncogenes and tumor suppressors. Dev Biol. 302:1–12. 2007. View Article : Google Scholar

9 

Chiyomaru T, Enokida H, Tatarano S, Kawahara K, Uchida Y, Nishiyama K, Fujimura L, Kikkawa N, Seki N and Nakagawa M: miR-145 and miR-133a function as tumour suppressors and directly regulate FSCN1 expression in bladder cancer. Br J Cancer. 102:883–891. 2010. View Article : Google Scholar : PubMed/NCBI

10 

Ichimi T, Enokida H, Okuno Y, Kunimoto R, Chiyomaru T, Kawamoto K, Kawahara K, Toki K, Kawakami K, Nishiyama K and Seki N: Identification of novel microRNA targets based on microRNA signatures in bladder cancer. Int J Cancer. 125:345–352. 2009. View Article : Google Scholar : PubMed/NCBI

11 

Hanke M, Hoefig K, Merz H, et al: A robust methodology to study urine microRNA as tumor marker: microRNA 126 and microRNA 182 are related to urinary bladder cancer. Urol Oncol. 28:655–661. 2010. View Article : Google Scholar

12 

Suzuki-Takahashi I, Kitagawa M, Saijo M, Higashi H, Ogino H, Matsumoto H, Taya Y, Nishimura S and Okuyama A: The interactions of E2F with pRB and with p107 are regulated via the phosphorylation of pRB and p107 by a cyclin-dependent kinase. Oncogene. 10:1691–1698. 1995.PubMed/NCBI

13 

Cordon-Cardo C, Wartinger D, Petrylak D, Dalbagni G, Fair WR, Fuks Z and Reuter VE: Altered expression of the retinoblastoma gene product: Prognostic indicator in bladder cancer. J Natl Cancer Inst. 84:1251–1256. 1992. View Article : Google Scholar : PubMed/NCBI

14 

Habuchi T, Ogawa O, Kakehi Y, Ogura K, Koshiba M, Sugiyama T and Yoshida O: Allelic loss of chromosome 17p in urothelial cancer: Strong association with invasive phenotype. J Urol. 148:1595–1599. 1992.PubMed/NCBI

15 

Esrig D, Elmajian D, Groshen S, Freeman JA, Stein JP, Chen SC, Nichols PW, Skinner DG, Jones PA and Cote RJ: Accumulation of nuclear p53 and tumor progression in bladder cancer. N Engl J Med. 331:1259–1264. 1994. View Article : Google Scholar : PubMed/NCBI

16 

Miyao N, Tsai YC, Lerner SP, Olumi AF, Spruck CH III, Gonzalez-Zulueta M, Nichols PW, Skinner DG and Jones PA: Role of chromosome 9 in human bladder cancer. Cancer Res. 53:4066–4070. 1993.PubMed/NCBI

17 

Kamb A, Gruis NA, Weaver-Feldhaus J, Liu Q, Harshman K, Tavtigian SV, Stockert E, Day RS III, Johnson BE and Skolnick MH: A cell cycle regulator potentially involved in genesis of many tumor types. Science. 264:436–440. 1994. View Article : Google Scholar : PubMed/NCBI

18 

Hecker N, Stephan C, Mollenkopf HJ, Jung K, Preissner R and Meyer HA: A new algorithm for integrated analysis of miRNA-mRNA interactions based on individual classification reveals insights into bladder cancer. PloS One. 8:e645432013. View Article : Google Scholar : PubMed/NCBI

19 

Fujita A, Sato JR, Rodrigues LO, Ferreira CE and Sogayar MC: Evaluating different methods of microarray data normalization. BMC Bioinformatics. 7:4692006. View Article : Google Scholar : PubMed/NCBI

20 

Smyth GK: Limma: Linear models for microarray data. Bioinformatics and computational biology solutions using R and Bioconductor. Gentleman R, Carey V, Dudoit S, Irizarry R and Huber W: Springer; New York: pp. 397–420. 2005

21 

Benjamini Y and Hochberg Y: Controlling the false discovery rate: A practical and powerful approach to multiple testing. J R Static Soc B Stat Methodol. 1:289–300. 1995.

22 

Wang X: miRDB: A microRNA target prediction and functional annotation database with a wiki interface. RNA. 14:1012–1017. 2008. View Article : Google Scholar : PubMed/NCBI

23 

Wang X and El Naqa IM: Prediction of both conserved and nonconserved microRNA targets in animals. Bioinformatics. 24:325–332. 2008. View Article : Google Scholar

24 

Betel D, Wilson M, Gabow A, Marks DS and Sander C: The microRNA.org resource: Targets and expression. Nucleic Acids Res. 36(Database): D149–D153. 2008. View Article : Google Scholar :

25 

Szklarczyk D, Franceschini A, Kuhn M, Simonovic M, Roth A, Minguez P, Doerks T, Stark M, Muller J, Bork P, et al: The STRING database in 2011: Functional interaction networks of proteins, globally integrated and scored. Nucleic Acids Res. 39(Database): D561–D568. 2011. View Article : Google Scholar :

26 

Smoot ME, Ono K, Ruscheinski J, Wang PL and Ideker T: Cytoscape 2.8: New features for data integration and network visualization. Bioinformatics. 27:431–432. 2011. View Article : Google Scholar :

27 

Zhang B, Kirov S and Snoddy J: WebGestalt: An integrated system for exploring gene sets in various biological contexts. Nucleic Acids Res. 33(Web Server): W741–W748. 2005. View Article : Google Scholar : PubMed/NCBI

28 

Duncan D, Prodduturi N and Zhang B: WebGestalt2: An updated and expanded version of the Web-based Gene Set Analysis Toolkit. BMC Bioinformatics. 11(Suppl 4): 102010. View Article : Google Scholar

29 

Neely LA, Rieger Christ KM, Neto BS, et al: A microRNA expression ratio defining the invasive phenotype in bladder tumors. Urol Oncol. 28:39–48. 2010. View Article : Google Scholar

30 

Wiklund ED, Bramsen JB, Hulf T, Dyrskjøt L, Ramanathan R, Hansen TB, Villadsen SB, Gao S, Ostenfeld MS, Borre M, et al: Coordinated epigenetic repression of the miR-200 family and miR-205 in invasive bladder cancer. Int J Cancer. 128:1327–1334. 2011. View Article : Google Scholar

31 

Haber DA: Splicing into senescence: The curious case of p16 and p19ARF. Cell. 91:555–558. 1997. View Article : Google Scholar : PubMed/NCBI

32 

Lloyd AC: p53: Only ARF the story. Nat Cell Biol. 2:E48–E50. 2000. View Article : Google Scholar : PubMed/NCBI

33 

Berggren P, Kumar R, Sakano S, et al: Detecting homozygous deletions in the CDKN2A(p16(INK4a))/ARF(p14(ARF)) gene in urinary bladder cancer using real-time quantitative PCR. Clin Cancer Res. 9:235–242. 2003.PubMed/NCBI

34 

Cordon-Cardo C: Molecular alterations associated with bladder cancer initiation and progression. Scand J Urol Nephrol Suppl. 42(s218): 154–165. 2008. View Article : Google Scholar

35 

Røtterud R, Nesland JM, Berner A and Fosså SD: Expression of the epidermal growth factor receptor family in normal and malignant urothelium. BJU Int. 95:1344–1350. 2005. View Article : Google Scholar : PubMed/NCBI

36 

Veerla S, Lindgren D, Kvist A, Frigyesi A, Staaf J, Persson H, Liedberg F, Chebil G, Gudjonsson S, Borg A, et al: MiRNA expression in urothelial carcinomas: Important roles of miR-10a, miR-222, miR-125b, miR-7 and miR-452 for tumor stage and metastasis, and frequent homozygous losses of miR-31. Int J Cancer. 124:2236–2242. 2009. View Article : Google Scholar : PubMed/NCBI

37 

Huang L, Luo J, Cai Q, Pan Q, Zeng H, Guo Z, Dong W, Huang J and Lin T: MicroRNA-125b suppresses the development of bladder cancer by targeting E2F3. Int J Cancer. 128:1758–1769. 2011. View Article : Google Scholar

38 

Blenkiron C and Miska EA: miRNAs in cancer: Approaches, aetiology, diagnostics and therapy. Hum Mol Genet. 16:R106–R113. 2007. View Article : Google Scholar : PubMed/NCBI

39 

Ivanovska I, Ball AS, Diaz RL, et al: MicroRNAs in the miR-106b family regulate p21/CDKN1A and promote cell cycle progression. Mol Cell Biol. 28:2167–2174. 2008. View Article : Google Scholar : PubMed/NCBI

40 

Hershko T and Ginsberg D: Up-regulation of Bcl-2 homology 3 (BH3)-only proteins by E2F1 mediates apoptosis. J Biol Chem. 279:8627–8634. 2004. View Article : Google Scholar

41 

Ginsberg D: E2F1 pathways to apoptosis. FEBS Lett. 529:122–125. 2002. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Jin N, Jin X, Gu X, Na W, Zhang M and Zhao R: Screening biomarkers of bladder cancer using combined miRNA and mRNA microarray analysis. Mol Med Rep 12: 3170-3176, 2015.
APA
Jin, N., Jin, X., Gu, X., Na, W., Zhang, M., & Zhao, R. (2015). Screening biomarkers of bladder cancer using combined miRNA and mRNA microarray analysis. Molecular Medicine Reports, 12, 3170-3176. https://doi.org/10.3892/mmr.2015.3739
MLA
Jin, N., Jin, X., Gu, X., Na, W., Zhang, M., Zhao, R."Screening biomarkers of bladder cancer using combined miRNA and mRNA microarray analysis". Molecular Medicine Reports 12.2 (2015): 3170-3176.
Chicago
Jin, N., Jin, X., Gu, X., Na, W., Zhang, M., Zhao, R."Screening biomarkers of bladder cancer using combined miRNA and mRNA microarray analysis". Molecular Medicine Reports 12, no. 2 (2015): 3170-3176. https://doi.org/10.3892/mmr.2015.3739
Copy and paste a formatted citation
x
Spandidos Publications style
Jin N, Jin X, Gu X, Na W, Zhang M and Zhao R: Screening biomarkers of bladder cancer using combined miRNA and mRNA microarray analysis. Mol Med Rep 12: 3170-3176, 2015.
APA
Jin, N., Jin, X., Gu, X., Na, W., Zhang, M., & Zhao, R. (2015). Screening biomarkers of bladder cancer using combined miRNA and mRNA microarray analysis. Molecular Medicine Reports, 12, 3170-3176. https://doi.org/10.3892/mmr.2015.3739
MLA
Jin, N., Jin, X., Gu, X., Na, W., Zhang, M., Zhao, R."Screening biomarkers of bladder cancer using combined miRNA and mRNA microarray analysis". Molecular Medicine Reports 12.2 (2015): 3170-3176.
Chicago
Jin, N., Jin, X., Gu, X., Na, W., Zhang, M., Zhao, R."Screening biomarkers of bladder cancer using combined miRNA and mRNA microarray analysis". Molecular Medicine Reports 12, no. 2 (2015): 3170-3176. https://doi.org/10.3892/mmr.2015.3739
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