Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
August-2015 Volume 12 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
August-2015 Volume 12 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Circulating microRNA‑122, ‑21 and ‑223 as potential markers of liver injury following warm ischaemia and reperfusion in rats

  • Authors:
    • Patrick Van Caster
    • Timo Brandenburger
    • Thorsten Strahl
    • Sabine Metzger
    • Inge Bauer
    • Benedikt Pannen
    • Sebastian Braun
  • View Affiliations / Copyright

    Affiliations: Department of Anaesthesiology, University Hospital Duesseldorf, Duesseldorf D‑40225, Germany, Biological and Medical Research Center, Heinrich Heine University, Duesseldorf D‑40225, Germany
  • Pages: 3146-3150
    |
    Published online on: May 7, 2015
       https://doi.org/10.3892/mmr.2015.3742
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The liver enzymes aspartate aminotransferase (AST), alanine aminotransferase (ALT) and lactate dehydrogenase (LDH) are commonly used but not specific markers to quantify hepatic injury. In this in vivo study it was determined whether hepatic expression and serum levels of the microRNAs (miRNA) miR‑122, ‑21 and ‑223 are altered and correlated with the release of liver enzymes after warm hepatic ischaemia and reperfusion (IR). Male Wistar rats were subjected to either 45 min of partial (70%) hepatic ischaemia and 240 min of reperfusion (n=7) or sham operation (n=5). Expression levels of miR‑122, ‑21 and ‑223 were analysed in serum and liver tissue by quantitative polymerase chain reaction and tested for correlation with serum activities of AST, ALT and LDH. The relative expression levels of circulating miR‑122 increased after IR and correlated with the serum activity of AST, ALT and LDH. Neither increased serum level of miR‑21 nor elevated relative hepatic expression of miR‑223 correlated with the serum activity of liver enzymes. The hepatic expression of miR‑122 was unaffected by IR. The correlation between circulating miR‑122 expression levels and liver enzyme activity qualifies miR‑122 as a potential biomarker of warm hepatic IR injury.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

Ozer J, Ratner M, Shaw M, Bailey W and Schomaker S: The current state of serum biomarkers of hepatotoxicity. Toxicology. 245:194–205. 2008. View Article : Google Scholar : PubMed/NCBI

2 

Mitchell PS, Parkin RK, Kroh EM, et al: Circulating microRNAs as stable blood-based markers for cancer detection. Proc Natl Acad Sci USA. 105:10513–10518. 2008. View Article : Google Scholar : PubMed/NCBI

3 

Lawrie CH, Gal S, Dunlop HM, et al: Detection of elevated levels of tumour-associated microRNAs in serum of patients with diffuse large B-cell lymphoma. Br J Haematol. 141:672–675. 2008. View Article : Google Scholar : PubMed/NCBI

4 

Xu J, Wu C, Che X, et al: Circulating microRNAs, miR-21, miR-122 and miR-223, in patients with hepatocellular carcinoma or chronic hepatitis. Mol Carcinog. 50:136–142. 2011. View Article : Google Scholar : PubMed/NCBI

5 

Tomimaru Y, Eguchi H, Nagano H, et al: Circulating microRNA-21 as a novel biomarker for hepatocellular carcinoma. J Hepatol. 56:167–175. 2012. View Article : Google Scholar

6 

Zhou J, Yu L, Gao X, et al: Plasma microRNA panel to diagnose hepatitis B virus-related hepatocellular carcinoma. J Clin Oncol. 29:4781–4788. 2011. View Article : Google Scholar : PubMed/NCBI

7 

Laterza OF, Lim L, Garrett-Engele PW, et al: Plasma MicroRNAs as sensitive and specific biomarkers of tissue injury. Clin Chem. 55:1977–1983. 2009. View Article : Google Scholar : PubMed/NCBI

8 

Antoine DJ, Dear JW, Lewis PS, et al: Mechanistic biomarkers provide early and sensitive detection of acetaminophen-induced acute liver injury at first presentation to hospital. Hepatology. 58:777–787. 2013. View Article : Google Scholar : PubMed/NCBI

9 

Ding X, Ding J, Ning J, et al: Circulating microRNA-122 as a potential biomarker for liver injury. Mol Med Rep. 5:1428–1432. 2012.PubMed/NCBI

10 

Shifeng H, Danni W, Pu C, Ping Y, Ju C and Liping Z: Circulating liver-specific miR-122 as a novel potential biomarker for diagnosis of cholestatic liver injury. PLoS One. 8:e731332013. View Article : Google Scholar : PubMed/NCBI

11 

Andersson P, Gidlöf O, Braun OO, Götberg M, van der Pals J, Olde B and Erlinge D: Plasma levels of liver-specific miR-122 is massively increased in a porcine cardiogenic shock model and attenuated by hypothermia. Shock. 37:234–238. 2012. View Article : Google Scholar

12 

Farid WR, Pan Q, van der Meer AJ, et al: Hepatocyte-derived microRNAs as serum biomarkers of hepatic injury and rejection after liver transplantation. Liver Transpl. 18:290–297. 2012. View Article : Google Scholar

13 

Yamada H, Suzuki K, Ichino N, et al: Associations between circulating microRNAs (miR-21, miR-34a, miR-122 and miR-451) and non-alcoholic fatty liver. Clin Chim Acta. 424:99–103. 2013. View Article : Google Scholar : PubMed/NCBI

14 

Kishimoto T, Eguchi H, Nagano H, et al: Plasma miR-21 is a novel diagnostic biomarker for biliary tract cancer. Cancer Sci. 104:1626–1631. 2013. View Article : Google Scholar : PubMed/NCBI

15 

Yu CH, Xu CF and Li YM: Association of MicroRNA-223 expression with hepatic ischemia/reperfusion injury in mice. Dig Dis Sci. 54:2362–2366. 2009. View Article : Google Scholar

16 

Chen X, Ba Y, Ma L, et al: Characterization of microRNAs in serum: a novel class of biomarkers for diagnosis of cancer and other diseases. Cell Res. 18:997–1006. 2008. View Article : Google Scholar : PubMed/NCBI

17 

Li Y, Jiang Z, Xu L, Yao H, Guo J and Ding X: Stability analysis of liver cancer-related microRNAs. Acta Biochim Biophys Sin (Shanghai). 43:69–78. 2011. View Article : Google Scholar

18 

Vickers KC, Palmisano BT, Shoucri BM, Shamburek RD and Remaley AT: MicroRNAs are transported in plasma and delivered to recipient cells by high-density lipoproteins. Nat Cell Biol. 13:423–433. 2011. View Article : Google Scholar : PubMed/NCBI

19 

Turchinovich A, Weiz L, Langheinz A and Burwinkel B: Characterization of extracellular circulating microRNA. Nucleic Acids Res. 39:7223–7233. 2011. View Article : Google Scholar : PubMed/NCBI

20 

Schmidt R, Tritschler E, Hoetzel A, et al: Heme oxygenase-1 induction by the clinically used anesthetic isoflurane protects rat livers from ischemia/reperfusion injury. Ann Surg. 245:931–942. 2007. View Article : Google Scholar : PubMed/NCBI

21 

Braun S, Plitzko G, Bicknell L, et al: Pretreatment with helium does not attenuate liver injury after warm ischemia reperfusion. Shock. 41:413–419. 2014. View Article : Google Scholar : PubMed/NCBI

22 

Pfaffl MW: A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res. 29:e452001. View Article : Google Scholar : PubMed/NCBI

23 

Lagos-Quintana M, Rauhut R, Yalcin A, Meyer J, Lendeckel W and Tuschl T: Identification of tissue-specific microRNAs from mouse. Curr Biol. 12:735–739. 2002. View Article : Google Scholar : PubMed/NCBI

24 

Wang K, Zhang S, Marzolf B, et al: Circulating microRNAs, potential biomarkers for drug-induced liver injury. Proc Natl Acad Sci USA. 106:4402–4407. 2009. View Article : Google Scholar : PubMed/NCBI

25 

Waidmann O, Bihrer V, Pleli T, et al: Serum microRNA-122 levels in different groups of patients with chronic hepatitis B virus infection. J Viral Hepat. 19:e58–e65. 2012. View Article : Google Scholar : PubMed/NCBI

26 

Cermelli S, Ruggieri A, Marrero JA, Ioannou GN and Beretta L: Circulating microRNAs in patients with chronic hepatitis C and non-alcoholic fatty liver disease. PLoS One. 6:e239372011. View Article : Google Scholar : PubMed/NCBI

27 

Xu J, Wu C, Che X, et al: Circulating MicroRNAs, miR-21, miR-122 and miR-223, in patients with hepatocellular carcinoma or chronic hepatitis. Mol Carcinog. 50:136–142. 2011. View Article : Google Scholar : PubMed/NCBI

28 

Landgraf P, Rusu M, Sheridan R, et al: A mammalian microRNA expression atlas based on small RNA library sequencing. Cell. 129:1401–1414. 2007. View Article : Google Scholar : PubMed/NCBI

29 

Ramkissoon SH, Mainwaring LA, Ogasawara Y, et al: Hematopoietic-specific microRNA expression in human cells. Leuk Res. 30:643–647. 2006. View Article : Google Scholar

30 

Honda M, Takeichi T, Asonuma K, Tanaka K, Kusunoki M and Inomata Y: Intravital imaging of neutrophil recruitment in hepatic ischemia-reperfusion injury in mice. Transplantation. 95:551–558. 2013. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Caster PV, Brandenburger T, Strahl T, Metzger S, Bauer I, Pannen B and Braun S: Circulating microRNA‑122, ‑21 and ‑223 as potential markers of liver injury following warm ischaemia and reperfusion in rats. Mol Med Rep 12: 3146-3150, 2015.
APA
Caster, P.V., Brandenburger, T., Strahl, T., Metzger, S., Bauer, I., Pannen, B., & Braun, S. (2015). Circulating microRNA‑122, ‑21 and ‑223 as potential markers of liver injury following warm ischaemia and reperfusion in rats. Molecular Medicine Reports, 12, 3146-3150. https://doi.org/10.3892/mmr.2015.3742
MLA
Caster, P. V., Brandenburger, T., Strahl, T., Metzger, S., Bauer, I., Pannen, B., Braun, S."Circulating microRNA‑122, ‑21 and ‑223 as potential markers of liver injury following warm ischaemia and reperfusion in rats". Molecular Medicine Reports 12.2 (2015): 3146-3150.
Chicago
Caster, P. V., Brandenburger, T., Strahl, T., Metzger, S., Bauer, I., Pannen, B., Braun, S."Circulating microRNA‑122, ‑21 and ‑223 as potential markers of liver injury following warm ischaemia and reperfusion in rats". Molecular Medicine Reports 12, no. 2 (2015): 3146-3150. https://doi.org/10.3892/mmr.2015.3742
Copy and paste a formatted citation
x
Spandidos Publications style
Caster PV, Brandenburger T, Strahl T, Metzger S, Bauer I, Pannen B and Braun S: Circulating microRNA‑122, ‑21 and ‑223 as potential markers of liver injury following warm ischaemia and reperfusion in rats. Mol Med Rep 12: 3146-3150, 2015.
APA
Caster, P.V., Brandenburger, T., Strahl, T., Metzger, S., Bauer, I., Pannen, B., & Braun, S. (2015). Circulating microRNA‑122, ‑21 and ‑223 as potential markers of liver injury following warm ischaemia and reperfusion in rats. Molecular Medicine Reports, 12, 3146-3150. https://doi.org/10.3892/mmr.2015.3742
MLA
Caster, P. V., Brandenburger, T., Strahl, T., Metzger, S., Bauer, I., Pannen, B., Braun, S."Circulating microRNA‑122, ‑21 and ‑223 as potential markers of liver injury following warm ischaemia and reperfusion in rats". Molecular Medicine Reports 12.2 (2015): 3146-3150.
Chicago
Caster, P. V., Brandenburger, T., Strahl, T., Metzger, S., Bauer, I., Pannen, B., Braun, S."Circulating microRNA‑122, ‑21 and ‑223 as potential markers of liver injury following warm ischaemia and reperfusion in rats". Molecular Medicine Reports 12, no. 2 (2015): 3146-3150. https://doi.org/10.3892/mmr.2015.3742
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team