Hepatitis B virus upregulates the expression of kinesin family member 4A

Zhu,Cheng‑Liang; Cheng,Duo‑Zhi; Liu,Fang; Yan,Xiao‑Hong; Wu,Kai‑Lang; Wang,Fu‑Bing; Liu,Xing‑Hui

doi:10.3892/mmr.2015.3792

September-2015 Volume 12 Issue 3

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September-2015 Volume 12 Issue 3

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Article

Hepatitis B virus upregulates the expression of kinesin family member 4A

Authors:
- Cheng‑Liang Zhu
- Duo‑Zhi Cheng
- Fang Liu
- Xiao‑Hong Yan
- Kai‑Lang Wu
- Fu‑Bing Wang
- Xing‑Hui Liu
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Affiliations: Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China, Department of Clinical Laboratory, Taihe Hospital of Hubei Medical College, Shiyan, Hubei 442000, P.R. China, The State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, Hubei 430072, P.R. China, Department of Clinical Laboratory, Zhongnan Hospital of Wuhan University, Wuhan, Hubei 430071, P.R. China, Department of Clinical Laboratory, Gongli Hospital, Second Military Medicine University, Shanghai 200135, P.R. China
Pages: 3503-3507
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Published online on: May 15, 2015

https://doi.org/10.3892/mmr.2015.3792
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Abstract

Hepatitis B virus (HBV) infection is one of the major causes of hepatocellular carcinoma (HCC). Kinesin family member 4A (KIF4A) is a microtubule‑based motor protein, which is upregulated in cervical and lung cancer. However, the expression of KIF4A in HBV‑associated HCC, and the effect of HBV on the expression of KIF4A remain to be elucidated. In the present study, the expression profiles of KIF4A were examined in cancerous tissues and paracancerous tissues from patients with HCC, who presented with histories of chronic HBV infection, and the role of HBV in the induction of the expression of KIF4A was investigated. HepG2 cells were transfected with the pHBV1.3, HBV infectious clone and a construct, which contained the luciferase gene under the control of the KIF4A gene promoter. The results demonstrated that the expression of KIF4A was significantly higher in the HCC tissues than in the paracancerous tissues. HBV activated the KIF4A gene promoter and upregulated the mRNA and protein expression of KIF4A. Furthermore, activation of the gene expression of KIF4A increased in a pHBV1.3 concentration‑dependent manner. These results provide novel insights into the understanding of HCC oncogenesis caused by HBV.

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1	Gomaa AI, Khan SA, Toledano MB, Waked I and Taylor-Robinson SD: Hepatocellular carcinoma: Epidemiology, risk factors and pathogenesis. World J Gastroenterol. 14:4300–4308. 2008. View Article : Google Scholar : PubMed/NCBI
2	Lim SG, Mohammed R, Yuen MF and Kao JH: Prevention of hepatocellular carcinoma in hepatitis B virus infection. J Gastroenterol Hepatol. 24:1352–1357. 2009. View Article : Google Scholar : PubMed/NCBI
3	Kim BK, Han KH and Ahn SH: Prevention of hepatocellular carcinoma in patients with chronic hepatitis B virus infection. Oncology. 81(Suppl 1): 41–49. 2011. View Article : Google Scholar
4	Nguyen VT, Law MG and Dore GJ: Hepatitis B-related hepatocellular carcinoma: epidemiological characteristics and disease burden. J Viral Hepat. 16:453–463. 2009. View Article : Google Scholar : PubMed/NCBI
5	Su CH, Lin Y and Cai L: Genetic factors, viral infection, other factors and liver cancer: an update on current progress. Asian Pac J Cancer Prev. 14:4953–4960. 2013. View Article : Google Scholar : PubMed/NCBI
6	Cougot D, Neuveut C and Buendia MA: HBV induced carcinogenesis. J Clin Virol. 34(Suppl 1): 75–78. 2005. View Article : Google Scholar
7	Zhang R, Cao Y, Bai L, et al: The collagen triple helix repeat containing 1 facilitates hepatitis B virus-associated hepatocellular carcinoma progression by regulating multiple cellular factors and signal cascades. Mol Carcinog. Sep 27–2014.Epub a head of print. View Article : Google Scholar
8	Martinez NW, Xue X, Berro RG, Kreitzer G and Resh MD: Kinesin KIF4 regulates intracellular trafficking and stability of the human immunodeficiency virus type 1 Gag polyprotein. J Virol. 82:9937–9950. 2008. View Article : Google Scholar : PubMed/NCBI
9	Wu G and Chen PL: Structural requirements of chromokinesin Kif4A for its proper function in mitosis. Biochem Biophys Res Commun. 372:454–458. 2008. View Article : Google Scholar : PubMed/NCBI
10	Ha MJ, Yoon J, Moon E, Lee YM, Kim HJ and Kim W: Assignment of the kinesin family member 4 genes (KIF4A and KIF4B) to human chromosome bands Xq13.1 and 5q33.1 by in situ hybridization. Cytogenet Cell Genet. 88:41–42. 2000. View Article : Google Scholar : PubMed/NCBI
11	Taniwaki M, Takano A, Ishikawa N, et al: Activation of KIF4A as a prognostic biomarker and therapeutic target for lung cancer. Clin Cancer Res. 13:6624–6631. 2007. View Article : Google Scholar : PubMed/NCBI
12	Wu G, Zhou L, Khidr L, et al: A novel role of the chromokinesin Kif4A in DNA damage response. Cell Cycle. 7:2013–2020. 2008. View Article : Google Scholar : PubMed/NCBI
13	Nunes Bastos R, Gandhi SR, Baron RD, Gruneberg U, Nigg EA and Barr FA: Aurora B suppresses microtubule dynamics and limits central spindle size by locally activating KIF4A. J Cell Biol. 202:605–621. 2013. View Article : Google Scholar : PubMed/NCBI
14	Tan YJ: Hepatitis B virus infection and the risk of hepatocellular carcinoma. World J Gastroenterol. 17:4853–4857. 2011. View Article : Google Scholar : PubMed/NCBI
15	Zhu C, Zhang R, Liu L, et al: Hepatitis B virus enhances interleukin-27 expression both in vivo and in vitro. Clin Immunol. 131:92–97. 2009. View Article : Google Scholar
16	Li Y, Xie J, Xu X, et al: Inducible interleukin 32 (IL-32) exerts extensive antiviral function via selective stimulation of interferon lambda1 (IFN-lambda1). J Biol Chem. 288:20927–20941. 2013. View Article : Google Scholar : PubMed/NCBI
17	Lu L, Wei L, Peng G, et al: NS3 protein of hepatitis C virus regulates cyclooxygenase-2 expression through multiple signaling pathways. Virology. 371:61–70. 2008. View Article : Google Scholar
18	Lee YM and Kim W: Association of human kinesin superfamily protein member 4 with BRCA2-associated factor 35. Biochem J. 374:497–503. 2003. View Article : Google Scholar : PubMed/NCBI
19	Gao J, Sai N, Wang C, et al: Overexpression of chromokinesin KIF4 inhibits proliferation of human gastric carcinoma cells both in vitro and in vivo. Tumour Biol. 32:53–61. 2011. View Article : Google Scholar
20	Narayan G, Bourdon V, Chaganti S, et al: Gene dosage alterations revealed by cDNA microarray analysis in cervical cancer: identification of candidate amplified and overexpressed genes. Genes Chromosomes Cancer. 46:373–384. 2007. View Article : Google Scholar : PubMed/NCBI

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Hepatitis B virus upregulates the expression of kinesin family member 4A

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