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Molecular and cellular mechanisms of tight junction dysfunction in the irritable bowel syndrome

  • Authors:
    • Peng Cheng
    • Jianning Yao
    • Chunfeng Wang
    • Lianfeng Zhang
    • Wuming Kong
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterology, First Hospital of Zhengzhou University, Zhengzhou, Henan 450052, P.R. China, Department of Gastroenterology, Affiliated Sixth People's Hospital of Shanghai Jiaotong University, Shanghai 201306, P.R. China
    Copyright: © Cheng et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 3257-3264
    |
    Published online on: May 21, 2015
       https://doi.org/10.3892/mmr.2015.3808
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Abstract

The pathophysiological mechanisms of the irritable bowel syndrome (IBS), one of the most prevalent gastrointestinal disorders, are complex and have not been fully elucidated. The present study aimed to investigate the molecular and cellular mechanisms of tight junction (TJ) dysfunction in IBS. Intestinal tissues of IBS and non‑IBS patients were examined to observe cellular changes by cell chemical tracer electron microscopy and transmission electron microscopy, and intestinal claudin‑1 protein was detected by immunohistochemistry, western blot analysis and fluorescence quantitative polymerase chain reaction. Compared with the control group, TJ broadening and the tracer extravasation phenomenon were observed in the diarrhea‑predominant IBS group, and a greater number of neuroendocrine cells and mast cells filled with high‑density particles in the endocrine package pulp as well as a certain extent of vacuolization were present. The expression of claudin‑1 in diarrhea‑predominant IBS patients was decreased, while it was increased in constipation‑predominant IBS patients. In conclusion, the results of the present study indicated that changes in cellular structure and claudin‑1 levels were associated with Tjs in IBS.
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Copy and paste a formatted citation
Spandidos Publications style
Cheng P, Yao J, Wang C, Zhang L and Kong W: Molecular and cellular mechanisms of tight junction dysfunction in the irritable bowel syndrome. Mol Med Rep 12: 3257-3264, 2015.
APA
Cheng, P., Yao, J., Wang, C., Zhang, L., & Kong, W. (2015). Molecular and cellular mechanisms of tight junction dysfunction in the irritable bowel syndrome. Molecular Medicine Reports, 12, 3257-3264. https://doi.org/10.3892/mmr.2015.3808
MLA
Cheng, P., Yao, J., Wang, C., Zhang, L., Kong, W."Molecular and cellular mechanisms of tight junction dysfunction in the irritable bowel syndrome". Molecular Medicine Reports 12.3 (2015): 3257-3264.
Chicago
Cheng, P., Yao, J., Wang, C., Zhang, L., Kong, W."Molecular and cellular mechanisms of tight junction dysfunction in the irritable bowel syndrome". Molecular Medicine Reports 12, no. 3 (2015): 3257-3264. https://doi.org/10.3892/mmr.2015.3808
Copy and paste a formatted citation
x
Spandidos Publications style
Cheng P, Yao J, Wang C, Zhang L and Kong W: Molecular and cellular mechanisms of tight junction dysfunction in the irritable bowel syndrome. Mol Med Rep 12: 3257-3264, 2015.
APA
Cheng, P., Yao, J., Wang, C., Zhang, L., & Kong, W. (2015). Molecular and cellular mechanisms of tight junction dysfunction in the irritable bowel syndrome. Molecular Medicine Reports, 12, 3257-3264. https://doi.org/10.3892/mmr.2015.3808
MLA
Cheng, P., Yao, J., Wang, C., Zhang, L., Kong, W."Molecular and cellular mechanisms of tight junction dysfunction in the irritable bowel syndrome". Molecular Medicine Reports 12.3 (2015): 3257-3264.
Chicago
Cheng, P., Yao, J., Wang, C., Zhang, L., Kong, W."Molecular and cellular mechanisms of tight junction dysfunction in the irritable bowel syndrome". Molecular Medicine Reports 12, no. 3 (2015): 3257-3264. https://doi.org/10.3892/mmr.2015.3808
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